In Vitro Anti-oxidant, Anti-fungal and Anti-staphylococcal Activity of Resveratrol-Producing Endophytic Fungi

  • Vagish Dwibedi
  • Sanjai SaxenaEmail author
Research Article


Trans-resveratrol is an enormously exploited stilbene exhibiting a broad spectrum of biological activities. However, commercial production to meet the demand and supply is very difficult as it is largely extracted from plants such as Polygonum cuspidatum making it very expensive. In the present study, resveratrol-producing endophytic fungi have been investigated for their in vitro antioxidant, antifungal and anti-staphylococcal activities. All the four fungal endophytes exhibited significant resveratrol production in the range of 31.3–89.1 mg/L in liquid cultures which was evaluated by high-performance liquid chromatography analysis. The endophytic isolate #12VVLPM in the present study exhibited strong antioxidant property in case of 2,2-diphenyl-1-picrylhydrazyl, Trolox equivalent antioxidant capacity, metal ion chelating, Nitric oxide radical and Hydrogen peroxide with IC50 values of 0.11–4.96, 0.28–2.16, 0.12–1.33, 0.08–1.35 and 0.12–1.18 mg/mL, respectively. The maximum inhibition (57.76 ± 0.82%) was exhibited by #12VVLPM against Botrytis cinerea. The present result shows strong correlation between antioxidant activities, anti-staphylococcal as well as antifungal activity.

Graphical Abstract


Endophytes In vitro antioxidant activity In vitro antifungal activity In vitro anti-staphylococcal activity Resveratrol 





Trolox equivalent antioxidant capacity


Ferric reducing antioxidant power


Total phenolic content


Total flavonoids content




Mueller Hinton agar


Sabouraud dextrose agar


Microbial type culture collection


American type culture collection


Staphylococcus aureus



The authors thank to the Department of Biotechnology (DBT), Government of India, New Delhi, for financial assistance through Project No-BT/PR9094/NDB/39/378/2013 and to Thapar Institute of Engineering and Technology, Patiala, Punjab, for providing the necessary infrastructure to carry out the research work. They also acknowledge Mr. Saurabh Rawat Assistant Manager, Sula Vineyards Pvt. Ltd. Nashik India for providing samples.

Author’s Contribution

Prof. SS conceived and designed the present study, while VD performed all experiments, analysed the data. Prof. SS and VD jointly wrote the publication.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest to publish this manuscript.


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Copyright information

© The National Academy of Sciences, India 2019

Authors and Affiliations

  1. 1.Department of BiotechnologyThapar Institute of Engineering and TechnologyPatialaIndia

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