Bubaline Aortic Matrix: Histologic, Imaging, Fourier Transform Infrared Spectroscopic Characterization and Application into Cattle Abdominal Hernia Repair
- 12 Downloads
Extracellular matrix (ECM) scaffolds have been shown to promote constructive remodeling response into a host. Minimal disruption of ECM ultrastructure during tissue processing is typically desirable. The purpose of the study was to prepare and characterize bubaline aortic matrix (BAM) for abdominal hernia repair in cattle. The bubaline aorta was decellularized with 1% sodium dodecyl sulfate (SDS) for 24 h followed by 0.25% trypsin for 2 h and again with 1% SDS for 24 h. Native and decellularized aortae were examined for residual cells, collagen structure and integrity by histologic examination and scanning electron microscopy (SEM). Masson’s trichrome and Weigert’s staining, DNA quantification and Fourier transform infrared (FTIR) spectroscopy were used for further characterization of BAM. BAM was used for repair of abdominal hernia in six cattle having mean weight of 132.50 ± 21.86 kg and hernial ring size of 40.55 ± 15.42 cm2. Clinical, hemato-biochemical and antioxidant parameters were evaluated to assess biocompatibility of xenogenic BAM. Histologically, the absence of cells and orderly arranged collagen fibers were observed in treated aorta. SEM confirmed the preservation of collagen structure and integrity. DNA content was significantly (P < 0.001) reduced in BAM (6.47 ± 1.26 ng/mg) as compared to the native aorta (488.11 ± 49.12 ng/mg). DNA extracts from BAM show marked removal of DNA material, with the absence of DNA band in agarose gel. FTIR spectrum of BAM showed all characteristic transmittance peaks of native aorta collagen indicating preserved collagen structure within decellularized aorta. Cattle with the BAM implant recovered uneventfully and remained sound. Hemato-biochemical and antioxidant findings were unremarkable. Bubaline aortic matrix shows excellent repair efficiency and biocompatibility for abdominal hernia repair in cattle without complications.
KeywordsBiocompatibility Bubaline aortic matrix Cattle DNA quantification FTIR spectroscopy Scanning electron microscopy
This paper includes a part of MVSc thesis of the first author approved by Junagadh Agricultural University (JAU) and was supported by the Director of Research, JAU, Gujarat (India).
Compliance with Ethical Standards
Conflict of interest
The authors declare that there is no conflict of interest to publish this manuscript.
- 1.Vilar JM, Corbera JA, Spinella G (2011) Double-layer mesh hernioplasty for repairing umbilical hernias in 10 goats. Turk J Vet Anim Sci 35:131–135Google Scholar
- 3.Kumar V, Kumar N, Gangwar AK, Singh H, Singh R (2015) Comparative histological and immunological evaluation of 1,4-butanediol diglycidyl ether crosslinked versus noncrosslinked acellular swim bladder matrix for healing of full-thickness skin wounds in rabbits. J Surg Res 197:436–446CrossRefGoogle Scholar
- 8.Baker MJ, Trevisan J, Bassan P, Bhargava R, Butler HJ, Dorling KM, Fielden PR, Fogarty SW, Fullwood NJ, Heys KA, Hughes C, Lasch P, Martin-Hirsch PL, Obinaju B, Sockalingum GD, Sule-Suso J, Strong RJ, Walsh MJ, Wood BR, Gardner P, Martin FL (2014) Using Fourier transform IR spectroscopy to analyze biological materials. Nat Protoc 8:1771–1791CrossRefGoogle Scholar
- 10.Kumar N, Devarathnam J, Sharma AK, Singh H, Kumar V, Gangwar AK, Maiti SK, Dewangan R (2010) Optimization of protocols for decellularization of aortic matrix of buffalo origin. In: 34th annual congress of Indian Society for Veterinary Surgery, Pondicherry, India, pp 117–118Google Scholar
- 19.Payne KJ, Veis A (1988) Fourier transform IR spectroscopy of collagen and gelatin solutions: deconvolution of the Amide I band for conformational studies. Biopolymers 387:1949–1960Google Scholar
- 22.Asodiya FA (2018) Clinical assessment of caprine acellular dermal matrix (CADM) for abdominal hernioplasty in buffaloes. MVSc Dissertation, Junagadh Agricultural UniversityGoogle Scholar
- 23.Vora SD, Kumar V, Asodiya FA, Singh VK, Fefar DT (2018) Bubaline diaphragm matrix: development and clinical assessment into cattle abdominal hernia repair. In: 42nd annual congress of Indian Society for Veterinary Surgery, Navsari, pp 187–188Google Scholar
- 24.Dewangan R, Sharma AK, Kumar N, Kumar A, Singh H, Kumar V, Maiti SK, Shrivastava S, Sonal Singh R (2013) Efficacy of bladder acellular matrix graft with and without cell seeding for cystoplasty in rabbits. Trends Biomater Artif Organs 27:47–57Google Scholar
- 25.Irvine SM, Cayzer J, Todd EM, Lun S, Floden EW, Negron L, Fisher JN, Dempsey SG, Alexander A, Hill MC, O’Rouke A, Gunningham SP, Knight C, Davis PF, Ward BR, May BCH (2011) Quantification of in vitro and in vivo angiogenesis stimulated by ovine forestomach matrix biomaterial. Biomaterial 32:6351–6361CrossRefGoogle Scholar
- 29.Kumar V, Gangwar AK, Kumar N, Singh H (2015) Use of the bubaline acellular diaphragm matrix for umbilical hernioplasty in pigs. Vet Arhiv 85:49–58Google Scholar
- 31.Preville X, Salvemini F, Giraud S, Chaufour S, Paul C, Stepien G, Ursini MV, Arrigo AP (1999) Mammalian small stress proteins protect against oxidative stress through their ability to increase glucose-6- phosphate dehydrogenase activity and by maintaining optimal cellular detoxifying machinery. Exp Cell Res 247:61–78CrossRefGoogle Scholar