UPLC–MS and Dereplication-Based Identification of Metabolites in Antifungal Extracts of Fungal Endophytes

  • Kanika ChowdharyEmail author
  • Nutan Kaushik
Research Article


Microbial biopesticides offer an eco-friendly alternative to synthetic chemical pesticides. Bioassay-guided fractionation of ethyl acetate concentrate of two fungal endophytes Setosphaeria rostrata (NCBI accession no. KR017047) and Acremonium sp. (NCBI accession no. KT428769) isolated from medicinal plants Chlorophytum borivilianum and Mentha piperita, respectively, was done in this study. These two isolates exhibited strong antifungal activities (IC50 value < 1 mg/ml) towards economically relevant phytopathogens, i.e. Sclerotinia sclerotiorum, Fusarium oxysporum, Botrytis cinerea and Rhizoctonia solani. Ethyl acetate extracts were subjected to MPLC to obtain fractions for metabolite identification. Bioactive components in the fractions were exhaustively characterised by aggressive dereplication based on accurate mass in UPLC–MS using online METLIN database. Systematically demarcated chromatographic and hyphenated spectroscopic techniques led to the identification of the well-described antifungal metabolite sulphamethazine (sulphonamide derivative). The study highlights the significance of dereplication procedure for probable identification of bioactive metabolites.


Endophytic fungi UPLC/MS Antifungal activity METLIN database Dereplication 



One of the authors humbly acknowledges the financial grant (JRF and SRF) of CSIR No. 09/550 (0037) 2009-EMR-1.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest to publish this manuscript.

Supplementary material

40011_2018_1060_MOESM1_ESM.docx (40 kb)
Supplementary material 1 (DOCX 40 kb)


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Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  1. 1.TERI UniversityDelhiIndia
  2. 2.TERI, India Habitat CenterNew DelhiIndia
  3. 3.Amity UniversityNoidaIndia

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