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Characterization of the Polysaccharides Released by the Toxic Marine Dinoflagellate Alexandrium catenella Under Metal Stress

  • Faouzi HerziEmail author
Research Article
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Abstract

A laboratory study was conducted in order to characterize the polysaccharides exudated by the marine toxic dinoflagellate Alexandrium catenella under metal stress conditions. Cultures were exposed to increasing free metal concentrations of copper ([Cu2+] = 2.51 × 10−9–1.27 × 10−8 mol/l), zinc ([Zn2+] = 2.80 × 10−7–1.00 × 10−6 mol/l), cadmium ([Cd2+] = 2.59 × 10−8–7.80 × 10−8 mol/l) and lead ([Pb2+] = 1.14 × 10−8–4.37 × 10−8 mol/l). The composition in aldoses of the polysaccharides exudated in the culture media was analyzed by gas chromatography. In comparison with the controls, the exudation of polysaccharides was greatly increased under metal stress, to reach its maximal values expressed as specific amounts in total aldoses at the highest levels of metal contamination (copper: 1.1 × 10−3 μg Cell−1 at 7.06 × 10−9 mol/l and 4.7 × 10−3 μg Cell−1 at 1.27 × 10−8 mol/l; lead: 1.15 × 10−3 μg Cell−1 at 2.6 × 10−8 mol/l). Composition in aldoses of the polysaccharides exudated was different according to the metal studied and to the contamination levels. Results showed that glucose represented the major aldose composing the polysaccharides released and being able to take part in metal speciation. The authors conclude that high levels of glucose released by A. catenella in its extracellular medium could take a part in some survival strategies setup by the dinoflagellate to adapt to metal stress.

Keywords

Alexandrium catenella Exudation Glucose Polysaccharides Trace metals 

Abbreviations

DOC

Dissolved organic carbon

DOM

Dissolved organic matter

FDOM

Fluorescent dissolved organic matter

GC

Gas chromatography

HABs

Harmful algal blooms

PARAFAC

Parallel factor analysis

PSP

Paralytic shellfish poisoning

Notes

Acknowledgements

The Conseil Général du Var (CG), Toulon Provence Méditerranée (TPM), supported this research and ARCUS CERES project (Région PACA-MAE). The authors are deeply indebted to Pr. Yves COLLOS, Drs. Estelle MASSERET and Mohamed LAABIR (ECOSYM UMR 5119 of the Montpellier 2 University) and to Dr. Dominique JAMET (PROTEE EA 3819) for providing them with the Alexandrium catenella strain ACT03 used in this study.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there is no conflict of interest to publish this manuscript.

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Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  1. 1.Université de ToulonLa GardeFrance

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