Genetic Variability and DNA Fingerprinting of Elite Mango Genotypes of India Using Microsatellite Markers
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Genetic variability of 20 promising Indian mango genotypes which include juicy and table types was studied employing 143 simple sequence repeats (SSRs). Of 143 SSRs, 80 were found to be polymorphic among the genotypes. Characterization of the 20 genotypes resulted in the detection of 236 alleles from 80 polymorphic loci with an average of 2.87 alleles per locus and polymorphism information content (PIC) of 0.63. UPGMA cluster analysis grouped all the genotypes into two major groups with a genetic similarity range of 37–66%. The genotypes were grouped into clusters based on the utility type. DNA fingerprinting of 20 genotypes was done using four hyper-variable SSRs, viz. SSR1, SSR11, SSR58 and SSR78, because of their unique cultivar-specific allele sizes that distinguish all the 20 popular mango genotypes by capillary electrophoresis. Cultivar-specific codes were developed based on allelic size of the amplified products for identification of the 20 genotypes. The study shows high genetic variability among promising genotypes and allele codes assigned for each mango genotype to confirm the genetic identity and genetic purity of variety. The authors conclude that this study developed unique allele-specific codes for the 20 mango genotypes in India.
KeywordsMango SSRs Genotypes DNA fingerprinting Polymorphism
The authors gratefully acknowledge the Department of Biotechnology (DBT), New Delhi, for the financial support.
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflict of interest to publish this manuscript.
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