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Genetic Variability and DNA Fingerprinting of Elite Mango Genotypes of India Using Microsatellite Markers

  • Malathi SurapaneniEmail author
  • Lakshminarayana R. Vemireddy
  • Begum Hameedunnisa
  • B. Purushotham Reddy
  • S. Y. Anwar
  • E. A. Siddiq
Research Article
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Abstract

Genetic variability of 20 promising Indian mango genotypes which include juicy and table types was studied employing 143 simple sequence repeats (SSRs). Of 143 SSRs, 80 were found to be polymorphic among the genotypes. Characterization of the 20 genotypes resulted in the detection of 236 alleles from 80 polymorphic loci with an average of 2.87 alleles per locus and polymorphism information content (PIC) of 0.63. UPGMA cluster analysis grouped all the genotypes into two major groups with a genetic similarity range of 37–66%. The genotypes were grouped into clusters based on the utility type. DNA fingerprinting of 20 genotypes was done using four hyper-variable SSRs, viz. SSR1, SSR11, SSR58 and SSR78, because of their unique cultivar-specific allele sizes that distinguish all the 20 popular mango genotypes by capillary electrophoresis. Cultivar-specific codes were developed based on allelic size of the amplified products for identification of the 20 genotypes. The study shows high genetic variability among promising genotypes and allele codes assigned for each mango genotype to confirm the genetic identity and genetic purity of variety. The authors conclude that this study developed unique allele-specific codes for the 20 mango genotypes in India.

Keywords

Mango SSRs Genotypes DNA fingerprinting Polymorphism 

Notes

Acknowledgements

The authors gratefully acknowledge the Department of Biotechnology (DBT), New Delhi, for the financial support.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest to publish this manuscript.

References

  1. 1.
    Mukerjee SK (1951) Origin of mango. J Genet Plant Breed 11:49–56Google Scholar
  2. 2.
    De Candolle A (1904) Origin of cultivated plants. Kegan Paul, TrenchGoogle Scholar
  3. 3.
    Singh RN (1996) Mango. Indian Council of Agricultural Research, PUSA, New DelhiGoogle Scholar
  4. 4.
    Karihaloo JL, Dwivedi YK, Archak S, Galkwab AB (2003) Analysis of genetic diversity of Indian mango cultivars using RAPD markers. J Hortic Sci Biotechnol 78:285–289CrossRefGoogle Scholar
  5. 5.
    Ravishankar KV, Lalitha A, Dinesh MR, Anand L (2000) Assessment of genetic relatedness among mango cultivars of India using RAPD markers. J Hortic Sci Biotechechnol 75(2):198–201CrossRefGoogle Scholar
  6. 6.
    Hemant Kumar NV, Narayanswamy P, Theertha Prasad D, Mukund GK, Sondur S (2001) Estimation of genetic diversity of commercial mango (Mangifera indica L.) cultivars using RAPD markers. J Hortic Sci Biotechechnol 76(5):529–533Google Scholar
  7. 7.
    Yamanaka N, Hasran M, He-Xu D, Tsunematsu H, Idris S, Ban T (2006) Genetic relationships and diversity of four Mangifera species revealed through AFLP analysis. Genet Res Crop Evol 53:949–954CrossRefGoogle Scholar
  8. 8.
    Eiadthong W, Yonemori K, Sugiura A, Utsunomiya N, Subhadrabandhu S (1999) Identification of mango cultivars of Thailand and evaluation of their genetic variation using the amplified fragments by simple sequence repeat (SSR) anchored primers. Sci Hortic 82:57–66CrossRefGoogle Scholar
  9. 9.
    Viruel M, Escribano P, Barbieri M, Ferri M, Hormaza J (2005) Fingerprinting, embryo type and geographic differentiation in mango (Mangifera indica L., Anacardiaceae) with microsatellites. Mol Breed 15(4):383–393CrossRefGoogle Scholar
  10. 10.
    Ravishankar KV, Hanumantha BM, Lalitha A, Dinesh MR (2011) Development of new microsatellite markers from Mango (Mangifera indica) and cross-species amplification. Am J Bot 98:96–99CrossRefGoogle Scholar
  11. 11.
    Malathi S, Lakshminarayan RV, Begum H, Purushotham RB, Neetasri C, Nagaraju J, Anwar SY, Siddiq EA (2013) Population structure and genetic analysis of different utility types of mango (Mangifera indica L.) germplasm of Andhra Pradesh state of India using microsatellite markers. Plant Syst Evol.  https://doi.org/10.1007/s00606-013-0790-1 CrossRefGoogle Scholar
  12. 12.
    Ravishankar KV, Padmakar B, Bajpai A, Srivastava N, Mani BH, Vasugi C, Rajan S, Dinesh MR (2015) Genetic diversity and population structure analysis of mango (Mangifera indica L.) cultivars assessed by microsatellite markers. Trees 29:775–783CrossRefGoogle Scholar
  13. 13.
    Jeffreys AJ, Wilson V, Thein SL (1985) Hypervariable ‘minisatellite’ regions in human DNA. Nature 314:647–673CrossRefGoogle Scholar
  14. 14.
    McCouch R, Chen X, Panaud O, Temnykh S, Xu Y, Cho YG, Huang N, Ishii T, Blair M (1997) Microsatellite marker development, mapping and applications in rice genetics and breeding. Plant Mol Biol 35:89–99CrossRefGoogle Scholar
  15. 15.
    Vemireddy L, Archak S, Nagarju J (2007) Capillary electrophoresis is essential for microsatellite marker based detection and quantification of adulteration of basmati rice (Oryza sativa L.). J Agric Food Chem 55:8112–8117CrossRefGoogle Scholar
  16. 16.
    Tagliar F, Bortolotti F (2006) Recent advances in the applications of CE to forensic sciences (2001–2004). Electrophoresis 27(1):231–243CrossRefGoogle Scholar
  17. 17.
    Altet L, Francino O, Sanchez A (2001) Microsatellite polymorphism in closely related dogs. J Hered 92(3):276–279CrossRefGoogle Scholar
  18. 18.
    Tang T, Huang J, Zhong Y, Shi S (2004) High-throughput S-SAP by fluorescent multiplex PCR and capillary electrophoresis in plants. J Biotechnol 114(1–2):59–68CrossRefGoogle Scholar
  19. 19.
    Porebski S, Bailey GL, Benard RB (1997) Modification of CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Report 15(1):8–15CrossRefGoogle Scholar
  20. 20.
    Anderson JA, Churchill GA, Autrique JE, Tanksley SD et al (1993) Optimizing parental selection for genetic linkage maps. Genome 36:181–186CrossRefGoogle Scholar
  21. 21.
    Rohlf FJ (1989) Numerical taxonomy and multivariate analysis system. Version 2.02i. Exeter Publishing, New YorkGoogle Scholar
  22. 22.
    Dinesh MR, Ravishankar KV, Sthapit B, Parthasarathy VA, Sandya BS, Nischita P, Lavanya B (2015) Genetic diversity studies in certain indigenous mango (Mangifera indica L.) varieties. Indian J Plant Genet Res 28(1):153–160CrossRefGoogle Scholar
  23. 23.
    Begum H, Reddy TM, Malthi S, Reddy BP, Archack S, Nagaraju J, Siddiq EA (2012) Molecular analysis for genetic distinctiveness and relationships of indigeneous landraces with popular cultivars of mango in Andhra Pradesh, India. Asian Aust J Plant Biotechnol 6(1):24–37Google Scholar
  24. 24.
    Adato A, Sharon D, Lavi U, Hillel I, Gazit S (1995) Application of DNA fingerprints for identification and genetic analysis of mango Mangifera indica genotypes. J Am Soc Hortic Sci 120:259–264CrossRefGoogle Scholar
  25. 25.
    Schnell RJ, Olano CT, Quintanilla WE, Meerow AW (2005) Isolation and characterization of 15 microsatellite loci from mango (Mangifera indica L.) and cross-species amplification in closely related taxa. Mol Ecol Notes 5:625–627CrossRefGoogle Scholar
  26. 26.
    Pandit SS, Mitra S, Giri AP, Pujari KH, Patil BP, Jambhale ND (2007) Genetic diversity analysis of mango cultivars using inter simple sequence repeat markers. Curr Sci 93:1135–1141Google Scholar

Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  • Malathi Surapaneni
    • 1
    Email author
  • Lakshminarayana R. Vemireddy
    • 2
  • Begum Hameedunnisa
    • 3
  • B. Purushotham Reddy
    • 3
  • S. Y. Anwar
    • 4
  • E. A. Siddiq
    • 1
  1. 1.Institute of BiotechnologyPJTSAUHyderabadIndia
  2. 2.Regional Research StationANGRAUTirupathiIndia
  3. 3.Y.S.R. Horticulture UniversityTadepalligudemIndia
  4. 4.Department of GeneticsOsmania UniversityHyderabadIndia

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