Potential Anticancer Activity of Caspian Cobra Venom Through Induction of Oxidative Stress in Glioblastoma Cell Line

  • Niloufar Sinaei
  • Abbas Zare Mirakabadi
  • Behzad Behnam
  • Azadeh Aminzadeh
  • Somayyeh Karami-MohajeriEmail author
Research Article


Despite advances in therapeutic strategies in the management of cancer, malignant glioma remains difficult to treat due to progressive resistance to conventional drugs. New studies made efforts to develop new anticancer agents from the screening of natural compounds. The biodiversity of venoms and their bioactive toxins makes them a special source for the development of novel therapeutic agents. The aim of the present study was to investigate the effect of Naja naja oxiana (NNO) crude venom on U87MG glioma cell line. Cellular viability and the generated amount of reactive oxygen species were determined by MTT and redox-sensitive dye DCFH-DA, respectively. A dose-dependent decline in viability of cells along with increase in generation of reactive oxygen species (ROS) occurred after the 24-h exposure to NNO venom. Incubation of RBC with NNO venom for 24 h indicated that hemolysis was not more than 6%. The results showed that NNO venom might act through the production of excess ROS, further disruption of mitochondrial function, and decrease in viability of U87MG without changes in the integrity of RBC membrane. However, more investigations are needed to find out detailed mechanisms by which NNO venom inhibits the viability of U87MG.


Caspian cobra venom Naja naja oxiana U87MG glioma cell line Cytotoxicity Reactive oxygen species 



The authors gratefully acknowledge the financial support (Project No.: 96000311) of the Kerman University of Medical Sciences.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  1. 1.Pharmaceutics Research Center, Institute of Neuropharmacology, Faculty of PharmacyKerman University of Medical SciencesKermanIran
  2. 2.Department of Venomous Animals and Antivenom ProductionRazi Vaccine and Serum Research InstituteHesarak, KarajIran

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