Chemotaxonomic Studies on Centella asiatica (L.) Urb. from Varied Phytogeographical Conditions of India for Its Industrial Prospection

  • Sharad SrivastavaEmail author
  • Surabhi Tiwari
  • Nishi Srivastava
  • Shikhar Verma
  • Ajay Kumar Singh Rawat
Research Article


Centella asiatica (L.) Urb. (Apiaceae) with high commercial value is an important herb in traditional Indian medicine. In the present study, comparative chemoprofiling was performed on various samples of C. asiatica (CA) collected from different phytogeographical zones of India, using high-performance thin-layer chromatography. Three biologically active phytochemicals, viz. asiaticosides, madeccasosides and asiatic acid, were investigated in 35 samples of CA. Variation in concentrations of asiaticoside, madeccasoside and asiatic acid was observed as 0.03–0.66%, 0.007–2.688% and 0.006–0.44%, respectively. The mobile phase which was used for asiaticoside and madecassoside was butanol:ethyl acetate:water, and for asiatic acid, it was toluene:ethyl acetate:formic acid. Among all samples, significant variation was observed in asiaticoside and madeccasoside from Northern Himalayas, while very small variation was found in asiatic acid from Deccan region. The results of the present study are of industrial importance in selection of phytogeographical regions of India for collection and to get maximum contents of active targeted phytochemicals.


Centella asiatica HPTLC Phytochemicals Traditional Indian medicine (TIM) 



The authors are thankful to the Director CSIR-NBRI, Lucknow, for his kind support and to facilitate the required resources during this study. They are also thankful to National Medicinal Plant Board, Ministry of AYUSH, for the financial support.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest to publish this manuscript.

Supplementary material

40011_2018_1021_MOESM1_ESM.doc (356 kb)
Supplementary material 1 (DOC 356 kb)


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Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  1. 1.Pharmacognosy and Ethnopharmacology DivisionCSIR-National Botanical Research InstituteLucknowIndia

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