Influence of Culture Media and Their Compositions on Haploid Induction in Indian Short Day Onion
- 15 Downloads
Two media, Gamborg’s medium (B5) and Murashige and Skoog’s medium (MS) with various combinations, were used to study their effect on haploid induction efficiency from unpollinated flowers or ovaries in tropical short day onion. In B5 medium, highest induction efficiency (1.9%) was observed on HAP18 followed by HAP17 and HAP05 whereas in MS medium, HAP40 recorded highest induction frequency (5.0%) followed by HAP32. Kinetin, meta-topolin, thidiazuron did not influence embryo induction. Sucrose at 7.5% in B5 medium and at 10% in MS medium was found to be optimum for induction. Days for plantlet induction were 98.9 ± 3.9 days in B5 medium and 83.1 ± 2.7 days in MS medium. Induction rate of 0.51% and survival of 27.8% in B5 medium and induction rate of 0.72% and survival of 33% in MS medium were observed. Three haploids, 1 mixoploid and 1 diploid in B5 medium and 2 haploids, one diploid in MS medium were obtained. Flow cytometry and cytology confirmed the status of haploid plants. MS medium was found to be better than B5 medium and newer combinations for haploid induction were identified. Plant Preservative Mixture (PPM™) at 1 mg/l was found to be efficient for inhibiting contamination from field grown flower buds. These combinations will be helpful in devising new strategies for faster and higher rate of haploid induction in tropical short day Indian cultivars.
KeywordsAllium cepa L. Gynogenesis Tropical onion Short day Murashige and Skoog’s medium Gamborg’s medium
This study was supported by the Department of Biotechnology, Government of India under the project code BT/PR12181/BPA/118/22/2014. The authors are thankful to the Director, ICAR-IARI and Head, Division of Vegetable Science, ICAR-IARI for providing the laboratory facilities and encouragement to carry out the work.
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflict of interest.
- 1.FAOSTAT (2014) Food and Agriculture Organization of the United Nations—FAO Statistical Database. http://www.fao.org/faostat/en/#data/QC. Assessed 3 June 2017
- 2.Singh RK, Bhonde SR (2011) Performance studies of exotic onion (Allium cepa L.) hybrids in the Nashik region of Maharashtra. Indian J Hill Farming 24:29–31Google Scholar
- 5.Muren RC (1989) Haploid plant induction from unpollinated ovaries in onion. HortScience 24:833–834Google Scholar
- 10.Jakše M, Hirschegger P, Bohanec B, Havey MJ (2010) Evaluation of gynogenic responsiveness and pollen viability of selfed doubled haploid onion lines and chromosome doubling via somatic regeneration. J Am Soc Hortic Sci 135:67–73Google Scholar
- 16.Anandhan S, Chavan AA, Gopal J, Mote SR, Shelke PV, Lawande KE (2014) Variation in gynogenic potential for haploid induction in Indian short-day onions. Indian J Genet 74:526–528Google Scholar
- 20.Chiancone B, Karasawa MMG, Gianguzzi V, Abdelgalel AM, Bárány I, Testillano PS, Marinoni DT, Botta R, Germanà MA (2015) Early embryo achievement through isolated microspore culture in Citrus clementina Hort. Ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’. Front Plant Sci 6:413CrossRefPubMedPubMedCentralGoogle Scholar
- 28.Bohanec B, Jakše M, Havey MJ (2003) Genetic analyses of gynogenetic haploid production in onion. J Am Soc Hortic Sci 128:571–574Google Scholar