Cytochrome-C Oxidase I Gene-Based Genetic Divergence and Molecular Phylogeny Among the Species of Fish Genus Channa
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The freshwater snakehead fishes are members of the family Channidae and order Perciform, which are native to parts of Africa and Asia with widespread distributions. The present study was aimed to assess the genetic variability and molecular phylogeny among 22 species of genus Channa, viz. C. argus, C. asiatica, C. aurantimaculata, C. bankanensis, C. barca, C. bleheri, C. burmanica, C. diplogramma, C. gachua, C. lucius, C. maculata, C. marulius, C. melasoma, C. micropeltis, C. orientalis, C. ornatipinnis, C. panaw, C. pleurophthlama, C. pulchra, C. punctata, C. stewartii and C. striata, using partial 513 bp long consensus sequence of cytochrome-c oxidase subunit I mitochondrial DNA retrieved from NCBI (March, 2018). The study showed 281 haplotypes with 267 variable sites (i.e., 33 singletons and 234 parsimoniously informative) in nucleotide positions. Most of the sequences were A + T rich, except in C. argus which is G + C rich (50.25%). The NJ and MP trees showed similar topology with discrete branches, each as a distinct species of the Channa with slight differences in bootstrap values. Maximum K2P distance was found between the C. ornatipinnis and the C. melasoma. The maximum numbers of polymorphic loci were present in C. gachua.
KeywordsChanna COI Genetic divergence mtDNA Phylogeny
The authors gratefully thank to ICAR-National Bureau of Fish Genetic Resources, Lucknow for providing the financial support and encouragement. The financial support for tissue sampling and DNA sequencing of fishes was taken under the institutional projects entitled ‘Genetic stock structure elucidation of Tenualosa ilisha and Channa striata using microsatellite, mitochondrial DNA and molecular cytogenetic markers’ and ‘DNA barcoding of India fishes,’ which is duly acknowledged.
Compliance with Ethical Standards
Conflict of interest
The authors declare that there is no conflict of interest to publish this manuscript.
- 1.Fowler HW (1934) Descriptions of new fishes obtained 1907 to 1910, chiefly in the Philippine islands and adjacent seas. Proc Acad Nat Sci Phila 85:233–367Google Scholar
- 2.Baisvar VS, Kumar R, Singh M, Singh AK, Chauhan UK, Mishra AK, Kushwaha B (2018) Genetic diversity analyses for population structuring in Channa striata using mitochondrial and microsatellite DNA regions with implication to their conservation in Indian waters. Meta Gene 16:28–38CrossRefGoogle Scholar
- 3.Hossain MK, Latifa GA, Rahman MM (2008) Observations on induced breeding of snakehead murrel, Channa striatus (Bloch, 1793). Int J Sustain Crop Prod 3:65–68Google Scholar
- 8.Meyer SC, Ross M, Nelson P, Chin P (2003) The Cambrian explosion: biology’s big bang. In: Campbell JA, Meyer SC (eds) Darwinism, design and public education. Michigan State University Press, Lansing, pp 323–402Google Scholar
- 11.Serrao NR, Steinke D, Hanner RH (2014) Calibrating snakehead diversity with DNA barcodes: expanding taxonomic coverage to enable identification of potential and established invasive species. PLoS ONE 10:9Google Scholar
- 13.Talwar PK, Jhingran AG (1992) Inland fishes of India and adjacent countries, vol 1. Balkema Publishers, Rotterdam, p 1158Google Scholar
- 14.Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- 19.Rozas J (2009) DNA sequence polymorphism analysis using DnaSP: pp 337–350. In Posada D (ed) Bioinformatics for DNA sequence analysis. Methods Mol Biol Series 537. Humana Press NJ, USAGoogle Scholar
- 22.Perrier X, Jacquemoud-Collet JP (2006) DARwin software. http://darwin.cirad.fr/. Accessed on 10 July 2016
- 25.Sharp PM, Tuohy TMF, Mosurski KR (1986) Codon usage in yeast: cluster analysis clearly differentiates between highly and lowly expressed genes. Nucleic Acids Res 14:125–5143Google Scholar
- 27.Dudu A, Georgescu SE, Popa O, Dinischiotu A, Costache M (2011) Mitochondrial 16S and 12S rRNA sequence analysis in four salmonid species from Romania. Acta Zool Acad Scient Hung 57:233–246Google Scholar
- 30.Yang F, Ding F, Chen H, He M, Zhu S, Ma X, Jiang L, Li H (2018) DNA barcoding for the identification and authentication of animal species in traditional medicine. Evid-Based Complement Altern Med 2018:1–18Google Scholar