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Cytochrome-C Oxidase I Gene-Based Genetic Divergence and Molecular Phylogeny Among the Species of Fish Genus Channa

  • Vishwamitra Singh Baisvar
  • Ravindra KumarEmail author
  • Mahender Singh
  • Basdeo Kushwaha
Research Article
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Abstract

The freshwater snakehead fishes are members of the family Channidae and order Perciform, which are native to parts of Africa and Asia with widespread distributions. The present study was aimed to assess the genetic variability and molecular phylogeny among 22 species of genus Channa, viz. C. argus, C. asiatica, C. aurantimaculata, C. bankanensis, C. barca, C. bleheri, C. burmanica, C. diplogramma, C. gachua, C. lucius, C. maculata, C. marulius, C. melasoma, C. micropeltis, C. orientalis, C. ornatipinnis, C. panaw, C. pleurophthlama, C. pulchra, C. punctata, C. stewartii and C. striata, using partial 513 bp long consensus sequence of cytochrome-c oxidase subunit I mitochondrial DNA retrieved from NCBI (March, 2018). The study showed 281 haplotypes with 267 variable sites (i.e., 33 singletons and 234 parsimoniously informative) in nucleotide positions. Most of the sequences were A + T rich, except in C. argus which is G + C rich (50.25%). The NJ and MP trees showed similar topology with discrete branches, each as a distinct species of the Channa with slight differences in bootstrap values. Maximum K2P distance was found between the C. ornatipinnis and the C. melasoma. The maximum numbers of polymorphic loci were present in C. gachua.

Keywords

Channa COI Genetic divergence mtDNA Phylogeny 

Notes

Acknowledgements

The authors gratefully thank to ICAR-National Bureau of Fish Genetic Resources, Lucknow for providing the financial support and encouragement. The financial support for tissue sampling and DNA sequencing of fishes was taken under the institutional projects entitled ‘Genetic stock structure elucidation of Tenualosa ilisha and Channa striata using microsatellite, mitochondrial DNA and molecular cytogenetic markers’ and ‘DNA barcoding of India fishes,’ which is duly acknowledged.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there is no conflict of interest to publish this manuscript.

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Copyright information

© The National Academy of Sciences, India 2019

Authors and Affiliations

  • Vishwamitra Singh Baisvar
    • 1
  • Ravindra Kumar
    • 1
    Email author
  • Mahender Singh
    • 1
  • Basdeo Kushwaha
    • 1
  1. 1.Molecular Biology and Biotechnology DivisionICAR-National Bureau of Fish Genetic ResourcesLucknowIndia

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