Role of Taurine in Cellular Volume Regulation in Erythrocytes of Air-Breathing Catfish (Clarias magur) Under Osmotic Stress
- 14 Downloads
The present study investigated the role of taurine in cellular volume regulation of erythrocytes isolated from freshwater air-breathing magur catfish (Clarias magur) under osmotic stress. Exposure of erythrocytes, pre-loaded with or without taurine, to hypotonic medium (− 80 mOsmol/L) led to a significant decrease in taurine level in the erythrocytes due to efflux of taurine through a band 3 transporter protein present in the plasma membrane with a slight increase in cellular volume of erythrocytes by 12–13%, whereas incubation of erythrocytes with hypertonic medium (+ 80 mOsmol/L) with taurine caused a significant uptake of taurine by the erythrocytes through the Na+-dependent pathway but without any loss of taurine from the erythrocytes which was accompanied by a slight decrease in the cellular volume of erythrocytes by 11–12%. Furthermore, a direct correlation between the osmosensitive cellular volume and taurine release could be established in the erythrocytes of magur catfish under hypotonic stress (r = 0.9921). In conclusion, the erythrocytes of air-breathing magur catfish do possess a very efficient taurine-dependent volume regulatory mechanism to resist the changes in cellular volume under anisotonic conditions as a unique adaptational strategy to defend against the osmosensitive changes in cellular volume of erythrocytes.
KeywordsHypotonicity Hypertonicity Anisotonicity Cellular water content 4,4′-Di-isothiocyanatostilbene-2,2′-disulphonic acid Band 3 protein transporter
This study was supported by a project sanctioned to the corresponding author by the Science and Engineering Research Board, New Delhi, and the DSA programme to the Department of Zoology, Shillong, by the University Grants Commission, New Delhi.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest to publish this manuscript.
- 1.Wehner F, Olsen H, Tinel H et al (2003) Cell volume regulation: osmolytes, osmolyte transport, and signal transduction. In: Reviews of physiology, biochemistry and pharmacology. Reviews of physiology, biochemistry and pharmacology, vol 148. Springer, Berlin, Heidelberg, pp 1–80Google Scholar
- 5.Haga Y, Kondo H, Kumagai A et al (2015) Isolation, molecular characterization of cysteine sulfinic acid decarboxylase (CSD) of red sea bream Pagrus major and yellowtail Seriola quinqueradiata and expression analysis of CSD from several marine fish species. Aquaculture 449:8–17. https://doi.org/10.1016/j.aquaculture.2015.04.004 CrossRefGoogle Scholar
- 7.Perlman DF, Goldstein L (1999) Organic osmolyte channels in cell volume regulation in vertebrates. J Exp Zool 283:725–733. https://doi.org/10.1002/(SICI)1097-010X(19990601)283:7%3c725:AID-JEZ10%3e3.0.CO;2-%23 CrossRefGoogle Scholar
- 12.Sen TK (1985) The fish fauna of Assam and the neighbouring North-Eastern States of India. In: Records of the zoological survey of India. Miscellaneous Publication, Calcutta, p 217Google Scholar
- 13.Banerjee B, Koner D, Lal P, Saha N (2017) Unique mitochondrial localization of arginase 1 and 2 in hepatocytes of air-breathing walking catfish, Clarias batrachus and their differential expression patterns under hyper-ammonia stress. Gene 622:13–22. https://doi.org/10.1016/j.gene.2017.04.025 CrossRefGoogle Scholar
- 15.Blaxhall PC, Daisley KW (1973) Routine haematological methods for use with fish blood. J Fish Biol 5:771–781. https://doi.org/10.1111/j.1095-8649.1973.tb04510.x CrossRefGoogle Scholar
- 17.Saha N, Dutta S, Häussinger D (2000) Changes in free amino acid synthesis in the perfused liver of an air-breathing walking catfish, Clarias batrachus infused with ammonium chloride: a strategy to adapt under hyperammonia stress. J Exp Zool 286:13–23. https://doi.org/10.1002/(SICI)1097-010X(20000101)286:1%3c13:AID-JEZ2%3e3.0.CO;2-X CrossRefGoogle Scholar
- 20.Saha N, Dutta S, Bhattacharjee A (2002) Role of amino acid metabolism in an air-breathing catfish, Clarias batrachus in response to exposure to a high concentration of exogenous ammonia. Comp Biochem Physiol Part B Biochem Mol Biol 133:235–250. https://doi.org/10.1016/S1096-4959(02)00145-8 CrossRefGoogle Scholar
- 29.Saha N, Goswami C (2004) Effects of anisotonicity on pentose-phosphate pathway, oxidized glutathione release and t-butylhydroperoxide-induced oxidative stress in the perfused liver of air-breathing catfish, Clarias batrachus. J Biosci 29:179–187. https://doi.org/10.1007/BF02703416 CrossRefGoogle Scholar
- 31.Goldstein L, Perlman DF (1995) Nitrogen metabolism, excretion, osmoregulation, and cell volume regulation. In: Walsh PJ, Wright P (eds) Nitrogen metabolism and excretion. CRC Press, Boca-Raton, pp 91–104Google Scholar
- 34.Motais R, Fiévet B, Borgese F, Garcia-Romeu F (1997) Association of the band 3 protein with a volume-activated, anion and amino acid channel: a molecular approach. J Exp Biol 200:361–367Google Scholar