Pharmacognostic Evaluation and Pharmacological Validation of Sphaeranthus indicus DC

  • Surabhi Tiwari
  • Pushpendra Kumar Shukla
  • Akanksha Srivastava
  • Manish Kumar
  • Ankita Misra
  • Sharad SrivastavaEmail author
Research Article


Sphaeranthus indicus is an important medicinal plant in Ayurveda which grows as weed in rice fields throughout India, Sri Lanka, Australia and Africa. The present study aimed for the pharmacognostical evaluation and pharmacological validation of this high-value medicinal species. All the pharmacognostical parameters were done as per the API guideline. A simple, rapid, sensitive and reproducible method was also developed for simultaneous HPTLC quantification of two bioactive compounds eugenol and β-sitosterol in S. indicus. The HPTLC was performed on silica gel 60 F254 by using toluene/ethyl acetate/glacial acetic acid (8:2:0.2) as a mobile phase for eugenol and β-sitosterol at Rf value 0.64 and 0.48, respectively. The content of eugenol (0.03%) and β-sitosterol (0.013%) was detected in dry weight per gram. In vitro activities viz. antioxidant, antidiabetic and anti-inflammatory were done to evaluate the pharmacological potential of S. indicus. Four antioxidant models viz. DPPH, ferric reducing power, 2-deoxyribose assay and antioxidant capacity were used to determine the free radical scanning. Antidiabetic and anti-inflammatory potential were determined by using starch–iodine colour assay and inhibition of protein denaturation model. IC50 value of S. indicus extract in DPPH and 2-deoxyribose assay was found (328.96 ± 0.003 µg/ml) and (30.36 ± 0.004 µg/ml), respectively. The present study will be helpful for quality check of the raw material and monitoring batch-to-batch consistency of herbal drugs, wherein S. indicus is used as an ingredient.


Sphaeranthus indicus Eugenol β-sitosterol HPTLC Antioxidant Antidiabetic 



The authors are thankful to the Director, CSIR-NBRI, India, for providing the facilities to conduct this research work.

Compliance with ethical standards

Conflict of interest

The authors do not have any conflict of interest to publish this manuscript.


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Copyright information

© The National Academy of Sciences, India 2019

Authors and Affiliations

  1. 1.Pharmacognosy DivisionCSIR-National Botanical Research InstituteLucknowIndia

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