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Modulation of Glycine Betaine Accumulation with Oxidative Stress Induced by Aluminium Toxicity in Rice

  • Subhankar Bera
  • Arnab Kumar De
  • Malay Kumar AdakEmail author
Research Article
  • 102 Downloads

Abstract

The present study addresses the sensitivity of rice species to varying concentrations of Al toxicity. Aluminium salt for plants was used in increasing order (240, 360, 480 µM) during short period to decipher the impact of metal stress on metabolic status with reference to oxidative damages. Interestingly, plants responded well with increase in linear root growth. In a dose dependent manner of metal concentration, plants suffered more from developed ROS (both O2 and H2O2) in root cortex. The histochemical detection of tissue lysis as detected by Evans blue and Hematoxylin was in proportionate to the aluminium concentration over control. In response to peroxide radical accumulated in the tissues, plants were characterized in a variable manner for APX, CAT and GR activities. Still, on protein polymorphism of these genes, the plants responded well with a distinct expression varied over control. In support of decreased activity, a single band expression was key feature to characterize the plants under Al toxicity. Plants though maintained a stable proportion of non-thiol content but a steeper up regulation of GR activity at highest concentration of Al was indicating for more GSH recruitment in oxidative stress. Banding patterns of APX, CAT and GR through Al concentrations appeared as bio-indices under metal reactivity in rice species. Betaine aldehyde dehydrogenase was also in proportionate manner to support the synthesis of osmolyte under metal toxicity. This is more relevant with protein expression of aldehyde dehydrogenase activity and distinct bands favor the gene expression under modulation of metal stress.

Keywords

Aluminium Betaine aldehyde dehydrogenase Glycine betaine ROS Rice Bio-indicator 

Abbreviation

APX

Ascorbate peroxidase

CAT

Catalase

GR

Glutathione reductase

ROS

Reactive oxygen species

H2O2

Hydrogen peroxide

O2

Superoxide

BADH

Betaine-aldehyde dehydrogenase

GB

Glycine betaine

2AP

2-acetyl pyrroline

NBT

Nitrotetrazolium blue chloride

DAB

3, 3′-Diaminobenzidine

PCA

Perchloric acid

PVP

Polyvinylpyrrolidone

EDTA

Ethylenediaminetetraacetic acid

DTT

Dithiothreitol

PMSF

Phenylmethylsulfonyl fluoride

TEMED

Tetramethylethylenediamine

NAD(P)H

Reduced Nicotinamide adenine dinucleotide phosphate

GSSG

Oxidized glutathione

DCPIP

2,6-Dichlorophenolindophenol

PMS

Phenazonium metho sulphate

MTT

3-(4,5- Dimethyl-2 thiazolyl 2,5 diphenyl-tetrazolium bromine)

ALDH

Aldehyde dehydrogenase

Notes

Acknowledgement

The present work was supported by DST-PURSE program activated to University of Kalyani and one of the authors acknowledges to UGC for Rajib Gandhi National Fellowship as a research fellow to conduct the work.

Compliance with Ethical Standards

Conflict of interest

The authors have no conflict of interest in publishing this research work.

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Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  • Subhankar Bera
    • 1
  • Arnab Kumar De
    • 1
  • Malay Kumar Adak
    • 1
    Email author
  1. 1.Plant Physiology and Molecular Biology Laboratory, Department of BotanyUniversity of KalyaniKalyani, NadiaIndia

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