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Releasing Digestive Enzymes by Brain Peptides in the Larvae of Pieris brassicae L. (Lepidoptera: Pieridae)

  • Ali Sharifloo
  • Arash ZibaeeEmail author
  • Jalal Jalali Sendi
  • Khalil Talebi Jahroumi
  • Abdolrashid Janghorbani
Research Article
  • 63 Downloads

Abstract

Neuropeptides synthesized by insect neurosecretory cells are engaged in several physiological process like immunity, feeding and reproduction so they deserve to be undertaken in subsequent experiments for discovery and manipulation regarding applied objectives. Due to importance of insect ability to utilize plant tissues, the present study was conducted to extract brain peptides in the fifth instar larvae of Pieris brassicae via High performance liquid chromatography and to identify their role in induction of digestive enzyme syntheses. Five peaks were determined and named as fractions 1, 2, 3, 4 and 5. The midguts of starved fifth larval instars were treated by each fraction of brain extract in vitro. The highest activities of α-amylase and lipase were found in the midgut treated by fractions 4 and 1, respectively while the fraction number 5 imposed the highest activities of glucosidases. Fraction 2 led to the highest activities of trypsin, chymotrypsin and elastase as the given serine proteinases as well as the two exopeptidases namely amino- and carboxypeptidases. Moreover, midgut treatments by different concentrations of TLCK (N-p-Tosyl-l-lysine methyl ester hydrochloride), TPCK (Tosyl phenylalanyl chloromethyl ketone), AEBSF (4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride) and Acarbose significantly decreased the activities of α-amylase, trypsin, chymotrypsin and elastase by the calculated IC50 values of 0.287, 0.439, 0.288 and 0.361 mM, respectively. The findings demonstrated that different neuropeptides would be engaged in enzymatic release of P. brassicae hence, manipulation of peptide receptors can change physiological process of insects and opens a selective strategy to pest control.

Keywords

Pieris brassicae Brain peptide Digestive enzyme High performance liquid chromatography Manipulation of peptide receptors 

Notes

Acknowledgements

The authors would like to appreciate assistances of Ms. Minoo Sajjadian for HPLC analysis. The present study was supported by University of Guilan, Iran.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there is no conflict of interest among the authors regarding publication of this manuscript.

Ethical Standard Statement

The experiments was conducted based on the rules on compliance with ethical statements.

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Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  1. 1.Department of Plant Protection, Faculty of Agricultural SciencesUniversity of GuilanRashtIran
  2. 2.Department of Plant Protection, College of Agriculture and Natural ResourcesUniversity of TehranKarajIran

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