A New Antifungal Antibiotic from Bacillus sp. KM5 Isolated from Rice Rhizospheric Soil
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Bacterial strain, Bacillus sp. KM5 was recently isolated and characterized by strong antifungal activities against several agronomically important filamentous pathogenic fungi including Rhizoctonia solani Nees. This is a new study to characterize four prominent antibiotics synthesized by Bacillus sp. KM5 (termed M1, M2, M3, and M4), isolated from rice rhizospheric soil. Lipopeptide profiling and spectroscopic characterizations of antibiotics M1 and M2 identified them as Bacillomycin F and Bacillomycin D, respectively. Antibiotic M3 was recognized as another iturinic lipopeptide Bacillopeptin A. Finally, M4 emerged as a novel straight chain polyhydroxy polyether compound with a single double bond, indicating a non-polyene structure with no prior mention in the literature. Furthermore, in vitro bioassay of M4 showed maximum activity against Rhizoctonia solani among five plant pathogenic fungi. This research could help in developing new or improved antifungal formulations or novel strategies to prevent fungal spoilage.
KeywordsAntibiotics Bacillomycin Bacillopeptin Bacillus Biopesticides
The authors are grateful for the financial and infrastructural support from Indian Agricultural Research Institute, New Delhi. The authors have no conflict of interest to declare.
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