Phytochemicals of Chrysophthalmum montanum (DC.) Boiss. Roots and Their Antiproliferative Activities Against HeLa and C6 Cell Lines
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Pre-fractionation and contemporary spectroscopic analysis provides important clues with regard to synergistic and antagonistic effects as well as biological activities in complex multi-mixtures, especially natural products. Phytochemical contents of the Chrysophthalmum montanum root extracts were analyzed by gas chromatography–mass spectrometry and high performance liquid chromatography–time of flight/mass spectrometry. Furthermore, biological activity of root extract was investigated by antiproliferative efficiencies against human uterus carcinoma and rat brain tumor cells in vitro. The biological activity tests were carried out as dose-dependent assay using a multiwell spectrophotometer. 5-Fluorouracil was used as standard anticancer drug. Total 61 lipophilic components were identified quantitatively from the sub-fraction of n-hexane in which saturated fatty acids (33.06%), unsaturated fatty acids (21.03%) and other organic components (43.35%). Twenty three hydrophilic components, 16 phenolic and 7 flavonoid compounds, were quantified in the hydrophilic sub-fractions obtained from the C. montanum roots. Among the hydrophilic components, the vanillic acid was analyzed as 46.463 ± 0.167 mg compound/g dry extract in the sub-fraction dichloromethane. The linoleic acid was analyzed as a substantial component (8.13%) in the sub-fraction n-hexane. The highest antiproliferative activities were obtained from the sub-fraction dichloromethane against HeLa cells (IC50 = 36.53 μg/mL) and the sub-fraction n-hexane against C6 cells (IC50 = 11.98 μg/mL). No cytotoxic effect was observed from these sub-fractions.
KeywordsHeLa C6 Antiproliferative activity Phytochemical HPLC–TOF/MS GC–MS
The authors acknowledge the financial support of the Scientific Research Project, Department of Bingol University (Projects: BAP-21-217-2014 and BAP-21-322-2015). They also thank Dr. Alpaslan Kocak for the identification of plant material and Prof. Dr. Nazlı Arda and Associate Prof. Ali Karagöz for providing C6 and HeLa cell lines. They also thank Mr. Serkan Koldas for grammatical revision.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
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