Cloning and Expression Analysis of 4-Coumarate CoA Ligase (4CL) Gene in Sugarcane Clones Varying in Lignin Content
- 113 Downloads
Sugarcane (Saccharum sp.) is one of the highest biomass producing plant, which is the best lignocellulosic feedstock for ethanol production. To achieve a more efficient conversion of biomass to ethanol, a better understanding of the main factors affecting biomass recalcitrance is needed. In the present study, the authors have cloned the partial coding sequence of 4-coumarate-CoA ligase (4CL) from an energy cane (EC 11010). The deduced amino acid sequence of this gene showed 98% similarity with the Zea mays protein and the phylogeny revealed clustering with the same gene from monocots. The quantitative expression of the 4CL gene among the four different sugarcane genotypes (EC 11010, IK 76-81, Co 86032 and CoC 671) showed higher level of transcripts in the energy cane EC 11010.
KeywordsLignocellulosic biomass Secondary cell wall Energy canes Real time polymerase chain reaction
This work has been supported by the Department of Science and Technology, Science Engineering and Research Board project No. SB/EMEQ-001/2013. The authors wish to thank Dr. M. N. Premachandran, Head, Division of Crop Improvement, and Dr. A. Selvi, Principle Scientist, Biotechnology Section and Head, Sugarcane Breeding Institute, Coimbatore for kindly providing sugarcane clones and extending laboratory facility for carrying out the work respectively.
Compliance with Ethical Standards
Conflict of interest
The authors declare that there is no conflict of interests regarding the publication of this paper.
- 6.CdeA Souza, Barbazuk B, Ralph SG, Bohlmann J, Hamberger B, Douglas CJ (2008) Genome-wide analysis of a land plant-specific acyl:coenzymeAsynthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella. New Phytol 179(4):987–1003Google Scholar
- 11.Steve R, Skaletsky JH (1998) Primer3. http://www.genome.wi.mit.edu/genome_software/other/primer3
- 16.Cao Y, Hu SL, Huang SX, Ren P, Lu XQ (2012) Molecular cloning, expression pattern, and putative cis-acting elements of a 4-coumarate:CoA ligase gene in bamboo (Neosino calamusaffinis). Electron J Biotechnol 15(5). doi: 10.2225/vol15-issue5-fulltext-10