Assessment of Circulating Biochemical Markers in Mice Receiving Cinnamon and Glycyrrhizin Under Carbon Tetrachloride Induced Hepatic Injury

  • Mahmood Rasool
  • Arif Malik
  • Sadia Saleem
  • Shakeel Ahmed Ansari
  • Javed Iqbal
  • Muhammad Asif
  • Mohammed Amjad Kamal
  • Mohammed Hussain Al-Qahtani
  • Sajjad KarimEmail author
Research Article


The study evaluated the hepatoprotective activity of plant extracts of cinnamon and glycyrrhizin in distinct dosage ways to minimize the oxidative stress induced by carbon tetrachloride (CCl4) in BALB/cJ inbred albino mice. Fifteen albino mice were divided into five groups, each group containing three mice. Group A was referred as positive control while group B, C, D and E were injected intraperitoneally with 1 mL/kg body weight of CCl4 twice a week for 1 month. Group C and D were treated orally with isolated extracts of cinnamon @50 mg/kg and glycyrrhizin @50 mg/kg respectively on daily basis for 1 month. However, group E was treated orally with combination dose of cinnamon @50 mg/kg + glycyrrhizin @50 mg/kg body weight. The increase in the levels of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP), triglyceride (TG), malondialdehyde (MDA) and glucose were recorded in CCl4 induced liver injury in mice while there is decrease in the levels of total protein (TP), reduced glutathione (GSH), Superoxide dismutase (SOD) and catalase (CAT) in CCL4 intoxicated mice. Isolated therapy of plant extracts of cinnamon and glycyrrhizin decreased the levels of ALT, AST, ALP, MDA, TG and glucose whereas increase in TP, GSH, SOD and CAT was observed in plant extracts treated mice. The best restoration of all the above said parameters near to control was observed in group of mice treated with combination dose of cinnamon and glycyrrhizin @50 mg/kg. Therefore, the present study declared the antioxidative, anti-inflammatory and hepatoprotective activity of standardized extracts of cinnamon and glycyrrhizin and their potent defensive property.


Hepatoprotective Inflammation Oxidative stress Liver function parameters Carbon tetrachloride toxicity 



Carbon tetrachloride


Reactive oxygen species


Reactive nitrogen species


Alanine transaminase


Aspartate transaminase


Alkaline phosphatase






Total protein


Reduced glutathione


Superoxide dismutase





This research work was funded and supported by Institute of Molecular Biology and Biotechnology, and Department of Pharmacy, The University of Lahore, Lahore, Pakistan. This project was partially supported by the National Plan for Science, Technology and Innovation (MAARIFAH) – King Abdulaziz City for Science and Technology - the Kingdom of Saudi Arabia – award number (10-BIO1073-03, 10-BIO1258-03 and 08-MED120-03).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Standard

This research work is approved by local ethical committee of university and the authors further declare that presently this manuscript is not submitted to any journal for publication consideration.


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Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  • Mahmood Rasool
    • 1
  • Arif Malik
    • 2
  • Sadia Saleem
    • 2
  • Shakeel Ahmed Ansari
    • 1
  • Javed Iqbal
    • 3
  • Muhammad Asif
    • 4
  • Mohammed Amjad Kamal
    • 5
  • Mohammed Hussain Al-Qahtani
    • 1
  • Sajjad Karim
    • 1
    Email author
  1. 1.Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical SciencesKing Abdulaziz UniversityJeddahKingdom of Saudi Arabia
  2. 2.Institute of Molecular Biology and BiotechnologyThe University of LahoreLahorePakistan
  3. 3.Department of PharmacyThe University of LahoreLahorePakistan
  4. 4.Department of Biotechnology and InformaticsBUITEMSQuettaPakistan
  5. 5.King Fahd Medical Research CenterKing Abdulaziz UniversityJeddahKingdom of Saudi Arabia

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