Doubled Haploidy Techniques in Wheat (Triticum aestivum L.): An Overview

  • Madhu PatialEmail author
  • Dharam Pal
  • Anjana Thakur
  • Ram Swaroop Bana
  • Sunny Patial


Wheat crop has a critical role in current food system and also in the future global food security. Global wheat demand in 2010 reached 666 million metric tons (MMT). If the demand growth rate remains constant, it has been predicted that the global wheat consumption would surpass 880 MMT by 2050. Fulfilling this demand needs new and more efficient wheat breeding methodologies. Conventional breeding has led to the development of number of varieties, but with the changing climatic regime accompanied with fast and continuous changing nature of biotic and abiotic stresses there is an urgent need to fasten the breeding methods. Hence, biotechnological tool like DH becomes an important weapon. The production of haploid plants from hybrids, followed by chromosome doubling will provide wheat breeder with a mean to accelerate the development of true breeding lines. Doubled haploid (DH) populations have lot of applications in plant breeding like cultivar and germplasm development, transferring traits from wild types, studying components of quantitative genetics and whole genome mapping. Among different DH production techniques, anther culture and Hordeum bulbosum have stronger genotypic specificity whereby, wide hybridization comes up with a solution. Amongst various wide hybridization techniques, DH production via Imperata cylindrica has been found to be the most economical and efficient. The genotypic nonspecific production lacks somaclonal variation and albino plants development alongwith having higher regeneration rate coupled with lower cost. Thus, integration of I. cylindrica mediated DH system with conventional breeding will be instrumental for future wheat breeding programmes.


Anther culture Haploid Imperata cylindrica Maize mediated DH Triticum aestivum 



The authors are thankful to Dr. K.V. Prabhu, JD (R), ICAR-IARI, New Delhi and Dr. H.K. Chaudhary, Head, CSK, Himachal Pradesh Krishi Vishvavidyalaya, Palampur for providing necessary help for initiation of I. cylindrica mediated DH work at ICAR-IARI, Regional Station, Shimla. Funding was provided by Indian Council of Agricultural Research.

Compliance with Ethical Standards

Conflict of interest

The authors do not have any conflict of interest to declare.


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Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  • Madhu Patial
    • 1
    Email author
  • Dharam Pal
    • 1
  • Anjana Thakur
    • 2
  • Ram Swaroop Bana
    • 3
  • Sunny Patial
    • 2
  1. 1.ICAR-Indian Agricultural Research Institute, Regional StationTutikandi CentreShimlaIndia
  2. 2.Chaudhary Sarvan Kumar Himachal Pradesh Krishi VishvavidyalyaPalampurIndia
  3. 3.ICAR-Indian Agricultural Research InstituteNew DelhiIndia

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