Advertisement

Acid Phosphatase and Vitamin A Induced Abnormal Tail Regeneration in Frog Tadpoles: an Immunohistochemical Study

  • Nikita Mahapatra
  • Sushil Kumar Dutta
  • Pravati Kumari Mahapatra
Research Article

Abstract

Application of vitamin A palmitate or retinoic acid causes abnormal tail regeneration and sometimes leads to formation of ectopic hind limbs along with pelvic girdles at the cut end of tail. Such a phenomenon referred to as homeotic transformation, has been reported in different anuran species. Acid phosphatase, a lysosomal marker enzyme is known to be associated with regeneration in amphibians. However, role of this enzyme during homeotic transformation of tail of an anuran tadpole into limbs is not explored. Thus, aim of the present study is immunohistochemical localization of acid phosphatase in non-amputated, normally regenerated and vitamin A treated abnormally regenerated tails (a prerequisite for ectopic organ development) of tadpoles of the Indian tree frog Polypedates maculatus. In the non-amputated and normally regenerated tails, acid phosphatase is majorly restricted to the epidermis and muscle patches although in the normally regenerated tails notochordal sheath and spinal cord also stain for acid phosphatase. In vitamin A treated tails, acid phosphatase is mostly localized in the epidermis, notochord precursor cells and undifferentiated cells of the mesenchyme. Notochordal cells and notochordal sheath also show positive staining. Since, acid phosphatase is majorly expressed by tissue forming precursor cells, this enzyme is suggested to be involved in tissue remodelling processes.

Keywords

Acid phosphatase Immunohistochemical localization Tail regeneration Vitamin A 

Notes

Acknowledgements

This work was supported by Department of Science and Technology (DST), Govt. of India (SR/SO/AS/41/2006). NM would like to thank DST, Govt. of India for a Junior Research Fellowship and University Grants Commission (UGC) for a research fellowship under UGC-BSR fellowship programme.

Conflict of interest

There is no conflict of interest among the authors.

References

  1. 1.
    Muller GB, Streicher J, Muller RJ (1996) Homeotic duplication of the pelvic body segment in regenerating tadpole tails induced by retinoic acid. Dev Genes Evol 206(5):344–348CrossRefPubMedGoogle Scholar
  2. 2.
    Bateson W (1894) Materials for the study of variation treated with especial regard to discontinuity in the origin of species. Macmillan Publications, LondonCrossRefGoogle Scholar
  3. 3.
    Kessel M, Gruss P (1991) Homeotic transformations of murine vertebrae and concomitant alteration of Hox codes induced by retinoic acid. Cell 67(1):89–104CrossRefPubMedGoogle Scholar
  4. 4.
    Mohanty-Hejmadi P, Dutta SK, Mahapatra P (1992) Limbs generated at site of tail amputation in marbled balloon frog after vitamin A treatment. Nature 355:352–353CrossRefPubMedGoogle Scholar
  5. 5.
    Maden M (1993) The homeotic transformation of tails into limbs in Rana temporaria by retinoids. Dev Biol 159(2):379–391CrossRefPubMedGoogle Scholar
  6. 6.
    Mahapatra PK, Mohanty-Hejmadi P (1994) Vitamin A-mediated homeotic transformation of tail to limbs, limb suppression and abnormal tail regeneration in the Indian jumping frog Polypedates maculatus. Dev Growth Differ 36(3):307–317CrossRefGoogle Scholar
  7. 7.
    Dutta SK (1994) Homeotic transformation in anurans. ISRAG Publications, BhubaneswarGoogle Scholar
  8. 8.
    Muller GB, Streicher J, Wurm R (1994) Structure and pattern in retinoid-induced ectopic limbs of anuran tadpoles. Paper presented to the 2nd World Congress of Herpetology, University of Adelaide, AustraliaGoogle Scholar
  9. 9.
    Das K, Dutta SK (1996) Further evidences of homeotic transformation in anuran tadpoles. Curr Sci 71(1):61–65Google Scholar
  10. 10.
    Maden M, Corcoran J (1996) Role of thyroid hormone and retinoid receptors in the homeotic transformation of tails into limbs in frogs. Dev Genet 19:85–93CrossRefPubMedGoogle Scholar
  11. 11.
    Love NR, Chen Y, Ishibashi S, Kritsiligkou P, Lea R, Koh Y, Gallop JL, Dorey K, Amaya E (2013) Amputation-induced reactive oxygen species are required for successful Xenopus tadpole tail regeneration. Nat Cell Biol 15:222–228CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Mahapatra PK, Mohanty-Hejmadi P, Chainy GBN (2002) Oxidative stress during vitamin A-induced abnormal tail regeneration in the tadpoles of Polypedates maculatus. Comp Biochem Physiol B 131(3):403–410CrossRefPubMedGoogle Scholar
  13. 13.
    Osborne PJ, Miller AT Jr (1963) Acid and alkaline phosphatase changes associated with feeding, starvation and regeneration in planarians. Biol Bull 124(3):285–292CrossRefGoogle Scholar
  14. 14.
    Coward SJ, Bennett CE, Hazlehurst BL (1974) Lysosomes and lysosomal enzyme activity in the regenerating planarian; Evidence in support of dedifferentiation. J Exp Zool 189:133–145CrossRefPubMedGoogle Scholar
  15. 15.
    Bowen ID, Den Hollander JE, Lewis GH (1982) Cell death and acid phosphatase activity in the regenerating planarian Polycelis tenuis Iijima. Differentiation 21(3):160–167CrossRefPubMedGoogle Scholar
  16. 16.
    Ghiretti F (1950) On the activity of acid- and alkaline phosphatase during tail regeneration in Triturus cristatus (Laur.). Experientia 6(3):98–100CrossRefGoogle Scholar
  17. 17.
    Schmidt AJ, Weary M (1963) Localization of acid phosphatase in the regenerating forelimb of the adult newt, Diemictylus viridescens. J Exp Zool 152:101–113CrossRefPubMedGoogle Scholar
  18. 18.
    Miller NR, Wolfe HJ (1968) The nature and localization of acid phosphatase during the early phases of urodele limb regeneration. Dev Biol 17(4):447–481CrossRefPubMedGoogle Scholar
  19. 19.
    Alibardi L (1998) Presence of acid phosphatase in the epidermis of the regenerating tail of the lizard (Podarcis muralis) and its possible role in the process of shedding and keratinisation. J Zool 246:379–390CrossRefGoogle Scholar
  20. 20.
    Ju BG, Kim WS (1994) Pattern duplication by retinoic acid treatment in the regenerating limbs of Korean salamander larvae, Hynobius leechii, correlates well with the extent of dedifferentiation. Dev Dyn 199:253–267CrossRefPubMedGoogle Scholar
  21. 21.
    Ju BG, Kim WS (2010) Lysosomal acid phosphatase mediates dedifferentiation in the regenerating salamander limb. Anim Cells Syst 14(2):73–81CrossRefGoogle Scholar
  22. 22.
    Gargioli C, Slack JMW (2004) Cell lineage tracing during Xenopus tail regeneration. Development 131(11):2669–2679CrossRefPubMedGoogle Scholar
  23. 23.
    Patnaik N, Mahapatra C, Dutta SK, Mahapatra PK (2012) Role of acid and alkaline phosphatases during vitamin A induced abnormal tail regeneration in the tadpoles of the Indian tree frog. Int J Curr Res 4(11):115–120Google Scholar
  24. 24.
    de Duve C (1983) Lysosomes revisited. Eur J Biochem 137:391–397CrossRefPubMedGoogle Scholar
  25. 25.
    Mohanty-Hejmadi P (1977) Care and management of amphibian embryos. Prakruti-Utkal Univ J Sci 11:81–87Google Scholar
  26. 26.
    Gosner KL (1960) A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica 16(3):183–190Google Scholar
  27. 27.
    Ho DM, Whitman M (2008) TGF-β signaling is required for multiple processes during Xenopus tail regeneration. Dev Biol 315(1):203–216CrossRefPubMedPubMedCentralGoogle Scholar
  28. 28.
    Slack JMW, Lin G, Chen Y (2008) The Xenopus tadpole: a new model for regeneration research. Cell Mol Life Sci 65:54–63CrossRefPubMedGoogle Scholar
  29. 29.
    Pati A, Dutta SK, Mahapatra PK (2003) Simultaneous induction of ectopic pelvic zone and duplication of regenerated limbs in tadpoles of Polypedates maculatus by vitamin A. Indian J Exp Biol 41:1424–1430PubMedGoogle Scholar
  30. 30.
    Setoguti T (1959) On acid phosphatase during lens regeneration in Triturus pyrrhogaster (BOIE). Arch histol Jpn 18(1):149–159CrossRefGoogle Scholar
  31. 31.
    Das P, Mohanty-Hejmadi P (1998) Histological effects of vitamin A on the tail amputated tadpoles of Bufo melanostictus. J Biosci 23(3):185–191CrossRefGoogle Scholar
  32. 32.
    Das P, Mohanty-Hejmadi P (1999) Histological effects of vitamin A on the tail-amputated tadpoles of Polypedates maculatus with special reference to homeotic transformation. Cells Tissues Organs 164(2):90–101CrossRefPubMedGoogle Scholar
  33. 33.
    Das P, Mohanty-Hejmadi P (2003) Vitamin A-mediated homeotic transformation and histological changes in amputated tail tissues of tadpoles of Rana tigerina. Curr Sci 84(4):557–562Google Scholar
  34. 34.
    Mahapatra PK, Mishra S, Dutta SK (2004) Histology of vitamin A induced ectopic limbs and normal hind limbs in tadpoles of Polypedates maculatus. Indian J Exp Biol 42(10):1007–1012PubMedGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  • Nikita Mahapatra
    • 1
  • Sushil Kumar Dutta
    • 1
  • Pravati Kumari Mahapatra
    • 1
  1. 1.Cell and Developmental Biology Laboratory, P.G. Department of ZoologyUtkal UniversityBhubaneswarIndia

Personalised recommendations