Production and Characterization of an Antifungal Compound from Pseudomonas protegens Strain W45

  • Apekcha BajpaiEmail author
  • Bijender Singh
  • Swati Joshi
  • Bhavdish Narain Johri
Research Article


Pseudomonas protegens strain W45 recovered from rhizosphere of wheat possesses potential to produce an antifungal compound in the culture medium. Therefore, to enhance its production, statistical optimization of medium was employed. Peptone, glycerol and incubation period were identified as significant variables affecting its production. These variables were further optimized by response surface methodology that resulted in 38% enhancement in inhibition zone with optimal values of 2.5%, 1.49% and 48 h for peptone, glycerol and incubation period, respectively. PCR amplification by gene specific primers for phloroglucinol, pyrrolnitrin and pyoluteorin resulted in amplicon of 745, 719 and 773 bp respectively, confirming the presence of all three genes. Antifungal compound was purified by thin layer chromatography. Gas chromatography mass spectrometry analysis of the methanolic extract reveals the presence of pyrrole type antifungal molecule 3-(2-methylpropyl)-hexahydropyrrolo [1,2-a]pyrazine-1,4-dione (C11H18N2O2). The compound significantly inhibited the growth of Sclerotinia sclerotiorum.


Pseudomonas protegens Optimization Antifungal compound Rhizobacteria Sclerotinia sclerotiorum 





Response surface methodology


Gas chromatography–mass spectrometry





This work is financially supported by the grants of National Academy of Sciences India, Allahabad to Prof. B.N. Johri (NASI Senior Scientist) at Department of Biotechnology, Barkatullah University, Bhopal, Madhya Pradesh, India. Help received by Prof. T. Satyanarayana (UDSC), New Delhi, India is gratefully acknowledged.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there is no financial/commercial conflict of interest.


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Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  • Apekcha Bajpai
    • 1
    Email author
  • Bijender Singh
    • 2
  • Swati Joshi
    • 3
  • Bhavdish Narain Johri
    • 1
  1. 1.Department of BiotechnologyBarkatullah UniversityBhopalIndia
  2. 2.Department of MicrobiologyMaharshi Dayanand UniversityRohtakIndia
  3. 3.Department of MicrobiologyUniversity of Delhi South CampusNew DelhiIndia

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