Chlorophyll Binding Ability of Non-chloroplastic DUF538 Protein Superfamily in Plants
- 80 Downloads
The type-1 water soluble chlorophyll binding proteins (WSCP1) have been generally known as chlorophyll extractors and transporters from the thylakoid membrane to the chloroplast envelope, where the membrane bound chlorophyllase catabolizes the chlorophyll. Despite the type-2 WSCP, WSCP1 has been known to be located in the chloroplasts of the green plants. In the present study, the non-chloroplastic protein superfamily containing domain of unknown function 538 (DUF538) as functional homologue of type-1 WSCP has been identified in plants. The structural similarities/differences and the cellular locations of Celosia cristata DUF538 and Chenopodium album WSCP1 were predicted by using computational tools and the chlorophyll binding abilities of their purified maltose binding protein-fused forms were estimated by maltose binding affinity method. It was predicted that despite CaWSCP1, CcDUF538 is a non-chloroplastic protein. The chlorophyll binding abilities of the recombinant fusion forms of test WSCP1 and DUF538 were estimated to be about 58 and 56%, respectively. Considering DUF538 as stress-induced protein, it was speculated that they may form complex with chlorophyll molecules or their hydrolyzed products out of chloroplasts to proceed the chlorophyll breakdown and nitrogen/carbon recycling in stress-challenged plants.
KeywordsCelosia Chenopodium Chlorophyll binding Water soluble
Water soluble chlorophyll binding proteins
Domain of unknown function
Bactericidal permeability increasing
Nitro blue tetrazolium
Ethylene diamine tetra acidic acid
The author of this paper is thankful to the Research Institute for Fundamental Sciences (RIFS), University of Tabriz for the financial support.
Compliance with Ethical Standards
Conflict of interest
There is no conflict of interest in this study.
- 6.Takahashi S, Yanai H, Oka-Takayama Y, Zanma-Sohtome A, Fujiyama K, Uchida A, Nakayama K, Satoh H (2013) Molecular cloning, characterization and analysis of the intracellular localization of a water-soluble chlorophyll binding protein (WSCP) from Virginia pepperweed (Lepidium virginicum), a unique WSCP that preferentially binds chlorophyll b in vitro. Planta 238:1065–1080CrossRefPubMedGoogle Scholar
- 14.Fang Z, Bouwkamp JC, Solomos T (1998) Chlorophyllase activities and chlorophyll degradation during leaf senescence in non-yellowing mutant and wild type of Phaseolus vulgaris L. J Exp Bot 49:503–510Google Scholar
- 23.Takahashi S, Yanai H, Nakamaru Y, Uchida A, Nakayama K, Satoh H (2012) Molecular cloning, characterization and analysis of the intracellular localization of a water-soluble chlorophyll-binding protein from Brussels sprouts (Brassica oleracea var. gemmifera). Plant Cell Physiol 53:879–891CrossRefPubMedGoogle Scholar