Investigating Disease Controlling Ability of Brassica Volatiles and Their Compatibility with Trichoderma harzianum

  • Pramod PrasadEmail author
  • J. Kumar
  • Shailesh Pandey
Research Article


The present investigation aimed to test the in vitro toxicity of Brassica volatiles against soil-borne plant pathogens (Rhizoctonia solani Kuhn, Sclerotium rolfsii Sacc., Fusarium oxysporum f.sp. ciceris (Padwick) Matuo & K. Sato and Sclerotinia sclerotiorum (Lib.) de Bary) and biocontrol fungus Trichoderma harzianum Rifai. Moreover, the response of T. harzianum to toxic volatiles was also studied in terms of glucanase and chitinase gene expression. A strong fungistatic effect of B. alba treatment was recorded against all the test pathogens. Noticeably, S. sclerotiorum manifested least sensitivity among all the tested pathogens. All T. harzianum isolates were less sensitive as compared to the assayed pathogens. Based on the in vitro study involving 6 T. harzianum isolates, T55 and Th-R showed least, while T39 showed highest sensitivity to the volatiles. Interestingly, the patterns relating to the effect of volatiles on inoculum density of T. harzianum isolates were similar to their effect on fungus growth in vitro. More importantly, expression of chitinase and glucanase genes in different Trichoderma isolates was up-regulated, which could improve the biocontrol activity of T. harzianum. Therefore, here the authors envisage that combining biocontrol and biofumigation has the potential to provide sustainable and cost-effective strategies to manage soil-borne plant pathogens.


Biofumigation Biocontrol Brassica Isothiocyanate Trichoderma Rhizoctonia 



The present study was undertaken as a part of the project entitled “Promoting IPM through a Common Minimum Programme in vegetable cultivation in Uttarakhand hills”, funded by Government of Uttarakhand (Letter No. 1757/Lekha-1/Ra-Kri. Yo./2008-09 dt. 10.07.2008). The authors are thankful to Government of Uttarakhand; Dean, College of Agriculture and Director Experiment Station, G.B.P.U.A.T., Pantnagar for their valuable assistance during this study.

Conflict of interest

The authors declare that there are no conflicts of interest regarding this manuscripts.


  1. 1.
    Methyl Bromide: Quarantine and Preshipment Uses.
  2. 2.
    Redovnikovic IR, Glivetic T, Delonga K, Vorkapic-furac J (2008) Glucosinolates and their potential role in plant. Periodicum Biologorum 11(4):297–309Google Scholar
  3. 3.
    Bones AM, Rossiter JT (1996) The myrosinase-glucosinolate system, its organisation and biochemistry. Physiol Plant 97:194–208CrossRefGoogle Scholar
  4. 4.
    Vig AP, Rampal G, Thind TS, Arora S (2009) Bio-protective effects of glucosinolates – A review. LWT - Food Science and Technology 42:1561–1572CrossRefGoogle Scholar
  5. 5.
    Matthiessen JN, Kirkegaard JA (2006) Biofumigation and Enhanced Biodegradation: opportunity and Challenge in Soil borne Pest and Disease Management. Crit Rev Plant Sci 25:235–265. doi: 10.1080/07352680600611543 CrossRefGoogle Scholar
  6. 6.
    Sarwar M, Kirkegaard JA,Wong PT Desmarchelier JM (1998) Biofumigation potential of brassicas. iii in vitro toxicity of isothiocyanates to soil borne fungal pathogens 201(1):103-112. doi: 10.1023/A:1004381129991
  7. 7.
    Griffiths HM, Gies D, Zitter TA (2011) Brassicas as Biofumigants for Controlling Soilborne Organisms in Potato Production for Upstate New York and Northern Pennsylvania
  8. 8.
    Lu P, Gilardi G, Gullino ML, Garibaldi A (2010) Biofumigation with Brassica plants and its effect on the inoculum potential of Fusarium yellows of Brassica crops. Eur J Plant Pathol 126(3):387–402CrossRefGoogle Scholar
  9. 9.
    Harman GE, Howell CR, Viterbo A, Chet I, Lorito M (2004) Trichoderma species-Opportunistic, avirulent plant symbionts. Nat Rev Microbiol 2:43–56CrossRefPubMedGoogle Scholar
  10. 10.
    Mukherjee PK, Horwitz BA, Herrera-Estrella A, Schmoll M, Kenerley CM (2013) Trichoderma Research in the Genome Era. Annu Rev Phytopathol 51:105–129. doi: 10.1146/annurev-phyto-082712-102353 CrossRefPubMedGoogle Scholar
  11. 11.
    Galletti S, Sala E, Leoni 0, Burzi PL, Cerato C (2008) Trichoderma spp. tolerance to Brassica carinata seed meal for a combined use in biofumigation. Biological Control 45(3):19-327. doi:  10.1016/j.biocontrol.2008.01.014
  12. 12.
    Sanchi S, Odorizzi S, Lazzeri L, Marciano P (2005) Effect of Brassica carinata seed meal treatment on the Trichoderma harzianum T39-Sclerotinia species interaction. Acta Hortic 698:287–292. doi: 10.17660/ActaHortic.2005.698.38 CrossRefGoogle Scholar
  13. 13.
    Smith BJ, Kirkegaard JA (2002) In vitro inhibition of soil microorganisms by 2-phenylethyl isothiocyanate. Plant Pathol 51:585–593CrossRefGoogle Scholar
  14. 14.
    Gallettia S, Fornasierb F, Cianchettaa S, Lazzeria L (2015) Soil incorporation of Brassica materials and seed treatment with Trichoderma harzianum: effects on melon growth and soil microbial activity. Ind Crops Prod 75:73–78. doi: 10.1016/j.indcrop.2015.04.030 CrossRefGoogle Scholar
  15. 15.
    Krassilnikov NA (1950) Actinomycetes-antagonists and antibiotic substances. S.R., Mascow, Leningrad, Academy of Sciences, U.S (in Russian) Google Scholar
  16. 16.
    Gil SV, Pastor S, March GJ (2009) Quantitative isolation of biocontrol agents Trichoderma spp., Gliocladium spp. and actinomycetes from soil with culture media. Microbiol Res 164(2):196–205. doi: 10.1016/j.micres.2006.11.022 CrossRefGoogle Scholar
  17. 17.
    Chakraborty BN, Chakraborty U, Saha A, Dey P, Sunar K (2010) Molecular Characterization of Trichoderma viride and Trichoderma harzianum Isolated from Soils of North Bengal Based on rDNA Markers and Analysis of Their PCR-RAPD Profiles. Global J Biotechnol Biochem 5(1):55–61Google Scholar
  18. 18.
    Prasad P, Kumar J (2012) An RNA isolation protocol for recovery of high quality functional RNA from fungi and plants. Curr Sci 102(9):1257–1260Google Scholar
  19. 19.
    Clarkson J, Michel V, Neilson R (2015) Mini-paper - Biofumigation for the control of soil-borne diseases.
  20. 20.
    Manici LM, Lazzeri L, Palmieri S (1997) In vitro fungitoxic activity of some glucosinolates and their enzyme-derived products toward plant pathogenic fungi. J Agric Food Chem 47(5):2768–2773. doi: 10.1021/jf9608635 CrossRefGoogle Scholar
  21. 21.
    Neubauer C, Heitmann B, Müller C (2014) Biofumigation potential of Brassicaceae cultivars to Verticillium dahliae. Eur J Plant Pathol 140(2):341–352. doi: 10.1007/s10658-014-0467-9 CrossRefGoogle Scholar
  22. 22.
    Rahmanpoura S, Backhousea D, Nonhebe HM (2009) Induced tolerance of Sclerotinia sclerotiorum to isothiocyanates and toxic volatiles from Brassica species. Plant Pathol 58:479–486. doi: 10.1111/j.1365-3059.2008.02015.x CrossRefGoogle Scholar
  23. 23.
    Kirkegaard JA, Simpfendorfer S, Holland J et al (2004) Effect of previous crops on crown rot and yield of durum and bread wheat in northern NSW. Aust J Agric Res 55(3):321–334. doi: 10.1071/AR03178 CrossRefGoogle Scholar
  24. 24.
    Haran S, Schickler H, Chet I (1996) Molecular mechanisms of lytic enzymes involved in the biocontrol activity of Trichoderma harzianum. Microbiology 142:2321–2331CrossRefGoogle Scholar
  25. 25.
    Ait-Lahsen H, Soler A, Rey M, de La Cruz J, Monte E, Llobell A (2001) An antifungal exo-alpha-1,3-glucanase (AGNI 13.1) from the biocontrol fungus Trichoderma harzianum. Applied. Environ Microbiol 67(12):5833–5839CrossRefGoogle Scholar
  26. 26.
    Ting ASY, Chai JY (2015) Chitinase and β-1,3-glucanase activities of Trichoderma harzianum in response towards pathogenic and non-pathogenic isolates: early indications of compatibility in consortium. Biocatalysis and Agricultural Biotechnology 4(1):110–113. doi: 10.1016/j.bcab.2014.10.003 CrossRefGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2016

Authors and Affiliations

  1. 1.Regional StationICAR-Indian Institute of Wheat and Barley ResearchShimlaIndia
  2. 2.College of AgricultureG.B. Pant University of Agriculture and TechnologyU.S. Nagar, PantnagarIndia
  3. 3.Rain Forest Research InstituteJorhatIndia

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