Exogenous Sulphydryl Improves Membrane Stabilization, Photosynthesis and Antioxidant Defense Systems in Vigna aconitifolia L. Under Water Stress
A 2-year (2010–2011) field experiment was conducted under hot arid environment at Bikaner, India to investigate the effects of exogenous sulphydryl (–SH) compounds (thioglycolic acid and thiourea) on the water relation, malondialdehyde (MDA) content, membrane stability, photosynthetic pigment contents, gas exchange parameters and antioxidant enzyme activities in moth bean (Vigna aconitifolia L.) under water limiting environment. Foliar application of –SH compounds significantly reduced MDA content and increased membrane stability, photosynthetic pigment content, gas exchange parameters and antioxidant enzyme activities. The –SH treated plants had 9–14, 10–14, 17–25, 16–74, 14–37% higher relative water content, membrane stability index, total chlorophyll content, antioxidant enzyme activities, and net photosynthetic rate (PN), respectively, whereas the MDA content was 12–21% lower, as compared to non-treated plants. Results suggest that under water limiting situation, exogenous –SH compound application improves photosynthesis, protects plants against oxidative damage by scavenging ROS and minimizing MDA content by elevated antioxidant enzyme activities. Results indicate that exogenous –SH application enhances the activities of antioxidant enzymes and maintains higher photosynthetic rate under water limiting environment and thus helps to improve drought tolerance in moth bean.
KeywordsAntioxidant enzymes Gas exchange parameters Sulphydryl compounds Water relation Vigna aconitifolia Yield
The authors wish to thank Director, CAZRI for providing funds and necessary facilities to carry out the study, and B. M. Yadav and Bharu Singh for their assistance in data collection and laboratory work. The facilities provided by In-charge, Plant Biotechnology Centre, SKRAU, Bikaner are duly acknowledged.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 3.Shao HB, Chu LY, Jaleel CA, Manivannan P, Panneerselvam R, Shao MA (2009) Understanding water deficit stress-induced changes in the basic metabolism of higher plants-biotechnologically and sustainably improving agriculture and the ecoenvironment in arid regions of the globe. Crit Rev Biotechnol 29:131–151CrossRefPubMedGoogle Scholar
- 9.Burman U, Garg BK, Kathju S (2002) Effect of spacing on seed yield and physiological traits in moth bean (Vigna aconitifolia). Indian J Agric Sci 72:76–79Google Scholar
- 15.Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplast. Plant Cell Physiol 22:867–880Google Scholar
- 20.Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research. Wiley, New YorkGoogle Scholar
- 22.Garg BK, Burman U, Kathju S (2006) Influence of thiourea on photosynthesis, nitrogen metabolism and yield of cluster bean (Cyamopsis tetragonoloba L.) under rainfed conditions of Indian arid zone. Plant Growth Regul 48:237–245Google Scholar
- 25.El-Tayeb MA (2006) Differential response of pigments, lipid per-oxidation, organic solutes, catalase and per-oxidase activity in the leaves of two Vicia faba L. cultivars to drought. Int J Agric Biol 8:116–122Google Scholar
- 29.Liu P, Li HY, Shang YL, Qi FG (2002) Effects of N-(1-naphtalene acetyl)-N′-(2-carboxy phenyl) thiourea (NCT) on senescent physiological activities of wheat. Acta Agron Boreali Sin 17:33–36Google Scholar
- 31.Prasad PVV, Staggenborg SA, Ristic Z (2008) Impacts of drought and/or heat stress on physiological, developmental, growth, and yield processes of crop plants. In: Ahuja LR, Reddy VR, Saseendran SA, Yu Q (eds) Response of crops to limited water: understanding and modelling water stress effects on plant growth processes, advances in agricultural systems modelling series 1. ASA, CSSA, SSSA, Madison, pp 301–355Google Scholar
- 33.Ramaswamy NK, Nathawat NS, Nair JS, Sharma HR, Kumawat SM, Singh G, Sahu MP, D’Souza SF (2007) Effect of seed soaking with sulphydryl compound on the photochemical efficiency and antioxidant defense system during the growth of pearl millet under water limiting environment. Photosynthetica 45:477–480CrossRefGoogle Scholar
- 34.Parihar GN, Sahu MP, Joshi NL (1998) Nitrogen, sulphur and thiourea nutrition of pearl millet (Pennisetum glaucum (L.) R. Br.). II. Effect on yield and yield components. Ann Arid Zone 37:59–67Google Scholar