Advertisement

The Detection Limit of PCR Amplification for Cryptosporidium spp. Oocysts in Fecal Samples

  • Harith Saeed Al-WaridEmail author
  • Ihsan M. Al-Saqur
  • Souhaila H. Mahmood
Short Communication
  • 3 Downloads

Abstract

Detection limits of PCR to detect Cryptosporidium spp. were determined in ninety-six preserved fecal samples (obtained for a different project). Small subunit ribosomal RNA gene was amplified from each sample. The best detection limit of the PCR method was > 150 oocysts.

Keywords

Cryptosporidium SSU rRNA Conventional PCR Detection limit 

Notes

References

  1. 1.
    Xiao L, Fayer R, Ryan U, Upton SJ (2004) Cryptosporidium taxonomy: recent advances and implications for public health. Clin Microbiol Rev 17:72–97CrossRefGoogle Scholar
  2. 2.
    Fayer R (2004) Cryptosporidium: a water-borne zoonotic parasite. Vet Parasitol 126:37–56CrossRefGoogle Scholar
  3. 3.
    Elsafi SH, Al-Maqati TN, Hussein MI, Adam AA, Hassan MMA, Al Zahrani EM (2013) Comparison of microscopy, rapid immunoassay, and molecular techniques for the detection of Giardia lamblia and Cryptosporidium parvum. Parasitol Res 112:1641–1646CrossRefGoogle Scholar
  4. 4.
    Ramirez NE, Sreevatsan S (2006) Development of a sensitive detection system for Cryptosporidium in environmental samples. Vet Parasitol 136:201–213CrossRefGoogle Scholar
  5. 5.
    Al-Warid HS, Mahmood S, Al-Saqur I (2012) Estimating the intensity of infection with Cryptosporidium spp. in Iraqi patients. Int J Rec Sci Res 3:894–896Google Scholar
  6. 6.
    Al-Warid HS, Al-Saqur IM, Mahmood SH (2012) Occurrence of Cryptosporidium spp. among people live in north of Baghdad. Eur J Sci Res 78:539–545Google Scholar
  7. 7.
    Meamar AR, Rezaian M, Rezaie S, Mohraz M, Mohebali M, Mohammad K, Golestan B, Guyot K, Cas E (2006) SSU-rRNA gene analysis of Cryptosporidium spp. in HIV positive and negative patients. Iran J Publ Health 35:1–7Google Scholar
  8. 8.
    Al-Warid HS (2014) The effect of freezing thawing on DNA extraction from Cryptosporidium oocysts in fecal samples. Int J Pharm Biol Sci 4:14–18Google Scholar
  9. 9.
    Ekanayake D, Arulkanthan A, Horadagoda NU, Sanjeevani GKM, Kieft R, Gunatilake S, Dittus WPJ (2006) Prevalence of Cryptosporidium and other enteric parasites among non-human primates in Polonnaruwa, Srilanka. Am J Trop Med Hyg 74:322–329CrossRefGoogle Scholar
  10. 10.
    Xiao L, Escalante A, Yang C, Sulaiman I, Escalante AA, Montali RJ, Fayer R, Lal AA (1999) Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl Environ Microbiol 65:1578–1583PubMedPubMedCentralGoogle Scholar
  11. 11.
    Nichols RA, Campbell BM, Smith HV (2006) Molecular fingerprinting of Cryptosporidium oocysts isolated during water monitoring. Appl Environ Microbiol 72:5428–5435CrossRefGoogle Scholar
  12. 12.
    Yu J, Lee S, Park W (2009) Comparative sensitivity of PCR primer sets for detection of Cryptosporidium parvum. Korean J Parasitol 47:293–297CrossRefGoogle Scholar
  13. 13.
    Lalonde LF, Gajadhar AA (2009) Effect of storage media, temperature, and time on preservation of Cryptosporidium parvum oocysts for PCR analysis. Vet Parasitol 160:185–189CrossRefGoogle Scholar
  14. 14.
    Goncalves EMN, Araujo RS, Orban M, Matte MH, Corbett CEP (2008) Protocol for DNA extraction of Cryptosporidium spp. oocyst in fecal sample. Rev Inst Med Trop Sao Paulo 50:165–167CrossRefGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2019

Authors and Affiliations

  • Harith Saeed Al-Warid
    • 1
    • 2
    Email author
  • Ihsan M. Al-Saqur
    • 3
  • Souhaila H. Mahmood
    • 1
  1. 1.Biology Department, College of ScienceUniversity of BaghdadBaghdadIraq
  2. 2.School of Natural ResourcesUniversity of MissouriColumbiaUSA
  3. 3.Al-Israa College UniversityBaghdadIraq

Personalised recommendations