Can Rumex hastatus D. Don. be Used as a Biocontrol Agent for Removing Lichens Colonizing Monuments? A Case Study from Kumaun Himalaya

  • Krishna Chandra
  • Yogesh JoshiEmail author
  • Shashi Upadhyay
  • Kapil Bisht
Short Communication


Role of lichens in deteriorating monuments constructed by stone artefact is quite well known, and till now various biocides and techniques have been used to remove them from monuments, all having some disadvantages at some level. In the present paper, the results and potential of leaf extract of Rumex hastatus D. Don. as a cost-effective, easy handling and eco-friendly biocontrol agent are being described.


Bio-remedial measure Lichen removal Monuments Scanning electron microscopy 



The authors would like to thank Scientific and Engineering Research Board (SB/FT/LS-313/2012) for providing financial assistance and to Wadia Institute of Himalayan Geology, Dehradun for allowing us to avail SEM facility. Thanks are also extended to Dr. Ramesh Chandra Upadhyay for identifying the rocks.

Compliance with ethical standards

Conflict of interest

All authors declare that there is no conflict of interest.


  1. 1.
    Warsheid T, Braams J (2000) Biodeterioration of stone: a review. Int Biodeterior Biodegrad 46:343–368. CrossRefGoogle Scholar
  2. 2.
    Chen J, Blume HP, Beyer L (2000) Weathering of rocks induced by lichen colonization—a review. CATENA 39:121–146. CrossRefGoogle Scholar
  3. 3.
    Lisci M, Monte M, Pacini E (2003) Lichens and higher plants on stone: a review. Int Biodeterior Biodegrad 51:1–17. CrossRefGoogle Scholar
  4. 4.
    Villar SEJ, Edwards HG, Seaward MRD (2004) Lichen biodeterioration of ecclesiastical monuments in northern Spain. Spectrochim Acta Part A 60:1229–1237. ADSCrossRefGoogle Scholar
  5. 5.
    De los Ríos A, Pérez-Ortega S, Wierzchos J, Ascaso C (2012) Differential effects of biocide treatments on saxicolous communities: case study of the Segovia cathedral cloister (Spain). Int Biodeterior Biodegrad 67:64–72. CrossRefGoogle Scholar
  6. 6.
    Monte M, Nichi D (1997) Effects of two biocides in the elimination of lichens from stone monuments: preliminary findings. Sci Technol Cult Herit 6:209–216Google Scholar
  7. 7.
    Bajpai PK, Bajpai RP, Chaterjee S, Singh A, Sinha GP (1999) Efficiency of five agricultural biocides on lichens eradications. In: Mukerjii KG, Chamola BP, Upreti DK, Upadhyaya RK (eds) Biology of lichens. Aravali Book International, New Delhi, pp 57–67Google Scholar
  8. 8.
    Gómez-Bolea A, Arino X, Balzarotti R, Saiz-Jimenez C (1999) Surface treatment of stones: consequences on lichenic colonization. In: Ciferri O, Tiano P, Mastromei G (eds) Of microbes and art: the role of microbial communities in the degradation and protection of cultural heritage, Florence, pp 233–237Google Scholar
  9. 9.
    Leavengood P, Twilley J, Asmus JF (2000) Lichen removal from Chinese spirit path figures of marble. J Cult Herit 1:71–74CrossRefGoogle Scholar
  10. 10.
    Ascaso C, Wierzchos J, Souza-Egipsy V, de los Rí A, Rodrigues JD (2002) In situ evaluation of the biodeteriorating action of microorganisms and the effects of biocides on carbonate rock of the Jeronimos Monastery (Lisbon). Int Biodeterior Biodegrad 49:1–12CrossRefGoogle Scholar
  11. 11.
    Morii M, Kawanobe W, Yamaji Y, Kashiwadani H (2009) Cleaning epiphytic vegetation with UV-C irradiation for buddhist images carved on natural cliff. Sci Conserv 48:21–31Google Scholar
  12. 12.
    Singh A, Upreti DK, Chatterjee S, Dhawan S (2002) Lichens and Indian monuments. In: Srivastava RB, Mathur GN, Agrawal OP (eds) Biodeterioration of materials-2. Defence Materials and Stores and Research and Development Establishment Kanpur, India, pp 128–131Google Scholar
  13. 13.
    Marin E, Vaccaro C, Leis M (2016) Biotechnology applied to historic stoneworks conservation: testing the potential harmfulness of two biological biocides. Int J Conserv Sci 7:227–238Google Scholar
  14. 14.
    Young ME, Alakomi HL, Fortune I et al (2002) Development of a biocidal treatment regime to inhibit biological growths on cultural heritage. Environ Geol 56:631–641. ADSCrossRefGoogle Scholar
  15. 15.
    Siano S, Salimbeni R (2010) Advances in laser cleaning of artwork and objects of historical interest: the optimized pulse duration approach. Acc Chem Res 43:739–750. CrossRefPubMedGoogle Scholar
  16. 16.
    Sanz M, Oujja M, Ascaso C, de los Ríos A, Pérez-Ortega S, Souza-Egipsy V, Wierzchos J, Speranza M, Cañamares MV, Castillejo M (2015) Infrared and ultraviolet laser removal of crustose lichens on dolomite heritage stone. Appl Surf Sci 346:248–255. ADSCrossRefGoogle Scholar
  17. 17.
    De Graef B, De Windt W, Dick J, Verstraete W, De Belie N (2005) Cleaning of concrete fouled by lichens with the aid of Thiobacilli. Mater Struct 38:875–882. CrossRefGoogle Scholar
  18. 18.
    Manandhar NP (1995) A survey of medicinal plants of Jajarkot District, Nepal. J Ethnopharmacol 48:1–6. CrossRefGoogle Scholar
  19. 19.
    Piervitori R (2004) Lichen and the biodeterioration of stonework: the Italian experiences. In: St Clair LL, Seaward MRD (eds) Biodeterioration of stone surface. Kluwer Academic Publishers, Dordrecht, pp 45–68CrossRefGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2019

Authors and Affiliations

  • Krishna Chandra
    • 1
  • Yogesh Joshi
    • 1
    • 2
    Email author
  • Shashi Upadhyay
    • 1
  • Kapil Bisht
    • 1
  1. 1.Lichenology Laboratory, Department of Botany, S.S.J. CampusKumaun UniversityAlmoraIndia
  2. 2.Lab. No. 15, Department of BotanyUniversity of RajasthanJaipurIndia

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