In Vitro Multiple Shoot Induction and Plant Regeneration from Male Ephedra foliata: A Potential Medicinal Gymnosperm
- 50 Downloads
Ephedra foliata Boiss. Ex. C.A. Mey, known as soma plant according to ancient Indian Ayurvedic system. It is known for its potential to cure many diseases. Review of literature revealed that E. foliata contains traces of alkaloids responsible for its therapeutic activity. In present study effect of different concentrations of plant growth regulator on nodal sector explants was checked for multiple shoot production. Maximum numbers of shoots were observed on Kinetin (2.5 mg/l) whereas, Kinetin (2.5 mg/l) with Indole acetic acid (2.5 mg/l) induced 9.04 ± 0.8 shoots in 22.46 ± 0.57 days and percentage of explants that showed response was more than any other combination. Out of the two plant growth regulators used for root induction from in vitro grown shoots 1-napthaleneacetic acid showed better response than indole-3-acetic acid.
KeywordsEphedra foliata Nodal sector explants Multiple shoots Growth regulators
First author thankful to his research guide as well as authorities of Savitribai Phule Pune University for providing necessary facilities for carrying out present study.
- 2.Dhiman, M, Sharma V (2010) Regeneration of plants from somatic embryos derived from stem culture in Ephedra foliata. In: Role of Biotechnology in Food Security and Climatic Change. Proceeding of sixth international plant tissue culture and biotechnology conference, 2010. Bangladesh Assoc. Plant Tissue Cult. & Biotech. Dhaka, Bangladesh, pp 19–25Google Scholar
- 3.Leung AY (1999) Ephedrine, Ephedra, Mahuang, Mahuanggen-what are they? Presented at AHPA Ephedra international symposium, Arlington, VAGoogle Scholar
- 4.Leung AY, Foster S (1996) Encyclopedia of common natural ingredients. Wiley, New YorkGoogle Scholar
- 6.Tanaka T, Ohba K, Kawahara K, Sakei E (1995) Comparison of the constituents of Ephedra herbs from various countries on ephedrine type alkaloids. Nat Med 49:418–424Google Scholar
- 7.Kondo N, Mikage M, Idaka K (1999) Medico-botanical studies of Ephedra plants from the Himalayan region, Part III. Causitive factors of variation of alkaloid content in herbal stems. Nat Med 53:194–200Google Scholar
- 8.Kasahara Y, Hayasaka H, Oba K, Hikino H (1986) Seasonal Dynamics of the accumulation of ephedrine alkaloids in Ephedra distachya herbs. Shoyakugazu 39:142–145Google Scholar
- 10.Razdan MK (1995) An introduction to plant tissue culture. Oxford and IBH publishing Co., Pvt. Ltd, New Delhi, pp 27–91Google Scholar
- 11.Gamborg OL, Philips GC (eds) (1998) Plant cell, tissue and organ culture fundamental methods. Narosa Publishing House, New Delhi, pp 87–95Google Scholar
- 14.Qazilbach NA (1971) Pakistani Ephedra. Pharmacol Weekbl 106:345–349Google Scholar
- 16.Gupta R, Sethi KL (1983) Conservation of medicinal plant resources in the Himalayan region. In: Jain SK, Mehra KL (eds) Conservation of tropical plant resources. In: Proceedings of workshop on conservation of tropical plant resources in South East Asia, New Delhi. Botanical Survey of India, Howrah, pp 101–109Google Scholar
- 20.Mousavi B, Saadat YA, Solouki M (2012) Factors affecting the shoot multiplication of Ephedra procera in iran. Trakia J Sci 10(1):16–21Google Scholar