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National Academy Science Letters

, Volume 42, Issue 1, pp 5–8 | Cite as

First Evidence of a Potential Antimicrobial Activity of Bacterial Laccase Against Various Plant Pathogens

  • Ambika VermaEmail author
  • Poonam Shirkot
  • Karuna Dhiman
  • Rashmi Sharma
  • Anjali Chauhan
Short Communication
  • 62 Downloads

Abstract

The present study deals with antimicrobial activity analysis of Pseudomonas putida LUA15.1 and its extracellular laccase, against six fungal plant pathogens viz., Fusarium oxysporum, Phytophthora capsici, Pythium aphanidermatum, Alternaria zinniae, Dematophora necatrix and Sclerotium rolfsii, and three bacterial pathogens viz., Ralstonia solanacearum, Xanthomonas axonopodis and Agrobacterium tumefaciens. Bacterial culture of Pseudomonas putida LUA15.1 and both crude and purified laccase enzyme preparations inhibited the mycelial growth and morphology of all six fungal pathogens. Both crude and purified laccase enzyme preparations showed variable zones of inhibition against three bacterial pathogens viz., Ralstonia solanacearum, Xanthomonas axonopodis and Agrobacterium tumefaciens. To the best of our knowledge this is the first report of successful control of various fungal and bacterial plant pathogens by bacterial laccase.

Keywords

Laccase Antimicrobial activity Fungal pathogens Bacterial pathogens 

Notes

Acknowledgements

The author acknowledges the financial assistance received from Department of Biotechnology at Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh to carry out the whole part of this research work.

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Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  • Ambika Verma
    • 1
    Email author
  • Poonam Shirkot
    • 1
  • Karuna Dhiman
    • 1
  • Rashmi Sharma
    • 2
  • Anjali Chauhan
    • 2
  1. 1.Department of BiotechnologyDr. Y. S. Parmar University of Horticulture and ForestryNauni, SolanIndia
  2. 2.Department of Basic Sciences (Microbiology Section)Dr. Y. S. Parmar University of Horticulture and ForestryNauni, SolanIndia

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