National Academy Science Letters

, Volume 41, Issue 5, pp 323–327 | Cite as

Optimized Densitometric Analysis for Determination of Triterpenoid Isomers in Vitex negundo L. Leaf

  • Sandeep R. Pai
  • Rajesh K. JoshiEmail author
Short Communication


Triterpenoid isomers oleanolic acid (OA) and betulinic acid (BA) were densitometrically separated and quantified using an optimized high performance thin layer chromatography analysis in Vitex negundo. Multiple wavelengths (400–675 nm at 25 nm increment) were scanned during the densitometric analysis, and 600 nm was found optimum to quantify OA and BA simultaneously. This technique allowed optimum separation of OA and BA with distinguishing band color and corresponding Rf than the previous reports. The leaf extract contained higher amount of BA (56.74 ± 2.83 ng/band) compared to OA (16.25 ± 0.81 ng/band). The method provides better separation, resolution at optimized wavelength for these compounds at lower concentration levels.


Vitex negundo High performance thin layer chromatography Densitometry Oleanolic acid Betulinic acid 



The authors are thankful to Indian Council of Medical Research (ICMR), New Delhi, India for necessary support. SRP is also indebted to Science and Engineering Research Board, Department of Science and Technology, New Delhi, for financial support (Grant No. SB/YS/LS-71/2013) during the work.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  1. 1.Department of Plant Biotechnology and Tissue Culture, Regional Medical Research CentreIndian Council of Medical ResearchBelagaviIndia
  2. 2.Department of Phytochemistry, Regional Medical Research CentreIndian Council of Medical ResearchBelagaviIndia
  3. 3.Amity Institute of BiotechnologyAmity UniversityPanvel, MumbaiIndia

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