Assessment of Physicochemical Characteristics and Thermophilic Bacterial Enumeration from Hot Water Springs of Himachal Pradesh
- 44 Downloads
Hot water springs are the geothermally heated and each thermal spring is found to be unique and different with respect to thermophilic bacterial diversity and various characteristics such as temperature, pH and chemistry of water. In the present study, pH and temperature of the six thermal springs assessed was found to vary from 6.5 to 8.4 and 36.5 to 94.6 °C respectively. Various physicochemical properties of these water samples evaluated were chloride (609.667–1857.667 mg/l), sulphate (12.667–44.667 mg/l), total hardness (34.833–131.600 mg/l), calcium hardness (29.00–125.00 mg/l), calcium carbonate hardness (58.00–272.33 mg/l) and magnesium content (3.85–26.15 mg/l). Evaluation of these six hot water springs was also carried out and thermophilic bacterial population was found to be highest (3.54 × 105 cfu/g) at Vashisht and minimum (0.54 × 105 cfu/g) at Kalath. In the present strudy most of the thermophilic bacterial isolates were found to belong to families Bacillaceae and Thermaceae.
KeywordsPhysicochemical analysis Enumeration Thermophilic bacterial population
The author acknowledges the financial assistance received from Department of Biotechnology at Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh to carry out the whole part of this work.
- 3.Sherpa MT, Das S, Thakur N (2013) Physicochemical analysis of hot water springs of Sikkim-Polok Tatopani, Borong Tatopani and Reshi Tatopani. Recent Res Sci Technol 5(1):63–67Google Scholar
- 4.Asir AHAP, Suresh NN (2013) Study of Physico–Chemical properties of Palvani Unhavare Euthermal Spring of Konkan Region, Maharashtra, India. Int J Pharm Chem Sci 2(4):1777–1780Google Scholar
- 5.APHA (1976) Standard methods for the examination of water and waste water. American Public Health 13th ed. Association, WashingtonGoogle Scholar
- 7.WHO (World Health Organization) (1998) Guidelines for drinking water quality, GenevaGoogle Scholar
- 8.www.idph state.il us/everhealth/pdf/drinkingwater/pdf (2011) Common found substances in drinking water and available treatment
- 9.Bhatia S, Batra N, Pathak A, Green SJ, Joshi A, Chauhan A (2015) Metagenomic evaluation of bacterial and archaeal diversity in the geothermal Hot Springs of Manikaran, India. Genome Announcements. Genomea Asm Org 3:1–2Google Scholar
- 10.Kumar M, Yadav AN, Tiwari R, Prasanna R, Saxena AK (2014) Evaluating the diversity of culturable thermotolerant bacteria from four hot springs of India. Biodivers Bioprospect Dev 1:1–9Google Scholar
- 12.Verma A, Gupta M, Shirkot P (2014) Isolation and characterization of thermophilic bacteria in natural hot water springs of Himachal Pradesh (India). Bioscan 9(3):947–952Google Scholar
- 13.Verma A, Shirkot P (2014) Purification and characterization of thermostable laccase from thermophilic geobacillus thermocatenulatus ms5 and its applications in removal of textile dyes. Sch Acad J Biosci 2(8):479–485Google Scholar