National Academy Science Letters

, Volume 41, Issue 4, pp 197–201 | Cite as

Estimating the Genetic Relationship in Various Warm Season Turfgrass Genotypes Using RAPD and ISSR Markers

  • Roshni AgnihotriEmail author
  • Shiv Lal Chawla
  • Vishal Srivashtav
Short Communication


The genetic diversity of 13 warm season turfgrass genotypes were studied in which 7 genotypes is of Cynodon dactylon and one each of C. dactylon × C. transvaalensis, Zoysia matrella, Zoysia tenuifolia, Stenotaphrum secundatum, Axonopus compressus and Eremochola ophiuroides were assessed using PCR based Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) markers. Out of 150 primers of RAPD and 18 primers of ISSR screened, six each of RAPD and ISSR primers, were used to generate polymorphism for all genotypes. The dendrogram obtained from UPGMA cluster analysis of Jaccard’s similarity values ranged from 0.029 to 0.395 for RAPD and from 0.085 to 0.750 for ISSR primers. Based on above markers the genotypes were grouped into six distinct clusters.


Turfgrass RAPD ISSR Genetic diversity Principal co-ordinate analysis 


  1. 1.
    Janakiram T, Namita N (2014) Genetic divergence analysis in turfgrasses based on morphological traits. Indian J Agric Sci 84(9):1035–1039Google Scholar
  2. 2.
    Souza SGH, Carpentieri-Pípolo V, Ruas CF, Carvalho VP (2008) Comparative analysis of genetic diversity among the maize inbred lines (Zea mays L.) obtained by studying genetic relationships in Lactuca spp. Theor Appl Genet 93:1202–1210Google Scholar
  3. 3.
    Kimball JA, Zuleta MC, Martin MC, Kenworthy KE, Chandra A, Milla-Lewis SR (2012) Assessment of molecular variation within ‘Raleigh’ St. Augustinegrass using amplified fragment length polymorphism markers. HortScience 47(7):839–844Google Scholar
  4. 4.
    Williams JGK, Kublelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphism’s amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Gupta S, Srivastav M, Mishra GP, Naik PK, Chauhan RS, Tiwari SK, Kumar M, Singh R (2008) Analysis of ISSR and RAPD markers for comparative analysis of genetic diversity among different Jatropha curcas genotypes. Afr J Biotechnol 7:4230–4243Google Scholar
  6. 6.
    Janakiram T, Namita N, Jain R (2015) Introduction to turfgrasses, 1st edn. Westville Publishing House, New DelhiGoogle Scholar
  7. 7.
    Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  8. 8.
    Rohlf FJ (2004) NTSYS-pc numerical taxonomy and multivariate analysis system, version 2.2. Exeter Software, Setauket, New YorkGoogle Scholar
  9. 9.
    Sneath PHA, Sokal RR (1973) Numerical taxonomy. The principle and practice of numerical classification. W.H. Freemon and Company, San FranciscozbMATHGoogle Scholar
  10. 10.
    Etmadi N, Sayed-tabatabaei BE, Zamanni Z, Razmjoo KH, Khalighi A, Lessani H (2006) Evaluation of diversity among Cynodon dactylon (L.) Pers. using RAPD markers. Int J Agric Biol 8(2):198–202Google Scholar
  11. 11.
    Riordan TP, Shazer SA, Johnson-Cicalese JM, Baxendale FP, Engleke MC, Gaussoin RE, Horst GL, Shearman RC (2007) Registration of ‘315’ buffalograss. Crop Sci 35:1206CrossRefGoogle Scholar
  12. 12.
    Assefa S, Taliaferro CM, Anderson MP, de Los Reyes BG, Edwards RM (1999) Diversity among Cynodon accessions and taxa based on DNA amplification fingerprinting. Genome 42:465–474CrossRefPubMedGoogle Scholar
  13. 13.
    Roodt R, Spies JJ, Burger TH (2002) Preliminary DNA fingerprinting of the turf grass Cynodon dactylon. Bothalia 32(1):117–122CrossRefGoogle Scholar
  14. 14.
    Wu L, Lin H (2004) Identifying buffalo-grass [Buchloe dactyloides (Nutt.) Engelm] cultivar breeding lines using Random Amplified Polymorphic DNA (RAPD) markers. J Am Soc Hortic Sci 119:126–130Google Scholar
  15. 15.
    Zhang X, Su D, Ma L (2007) Analysis of genetic diversity in buffalograss determined by random amplified polymorphic DNA markers. HortScience 42(3):474–477Google Scholar

Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  1. 1.Department of Floriculture and LandscapingDr. Y. S. Parmar University of Horticulture and ForestryNauni, SolanIndia
  2. 2.Department of Floriculture and Landscape Architecture, ASPEE College of Horticulture and ForestryNavsari Agricultural UniversityNavsariIndia
  3. 3.Division of GeneticsICAR-IARINew DelhiIndia

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