Potential of different salt forming agents on the formation of chitosan nanoparticles as carriers for protein drug delivery systems
- 97 Downloads
The effects of salt forming agents for chitosan on the potential for nanoparticle formation was investigated. The salt forms were prepared from the amino acid group, including glutamic and aspartic acids, and the alpha hydroxyl acid group, including lactic and glycolic acids. All types of chitosan salt could be used to prepare bovine serum albumin (BSA) loaded nanoparticles. The chitosan salts prepared from the amino acid group showed a higher salt formation ability as demonstrated using FTIR, hence a higher %encapsulation efficiency (%EE) and a reduction in zeta potential were obtained. The difference was due to the different organic acids used giving different polymer conformations and pH values in solution. Chitosan glutamate gave the highest salt formation ability and hence the highest %EE was obtained. The release of protein from all types of chitosan was similar and chitosan glutamate exhibited the highest release. Chitosan salt is therefore a material of choice for protein-loaded nanoparticles and the characteristics of nanoparticles can be readily modulated by different types of salt form.
KeywordsChitosan salt Chitosan nanoparticle Protein Amino acid and alpha-hydroxyl acid
This work was supported by the Research and Development Institute of Silpakorn University. This project would have been impossible without the facilities provided by the Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University.
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
- Boonsongrit Y, Mueller BW, Mitrevej A (2008) Characterization of drug-chitosan interaction by 1H NMR, FTIR and isothermal titration calorimetry. Eur J Pharm Sci 69(1):388–395Google Scholar
- Lowry O, Rosebrough N, Farr A, Randall R (1951) Protein measurement with the folin–phenol reagents. J Biol Chem 193:265–275Google Scholar