Journal of Pharmaceutical Investigation

, Volume 49, Issue 1, pp 37–44 | Cite as

Potential of different salt forming agents on the formation of chitosan nanoparticles as carriers for protein drug delivery systems

  • Manee Luangtana-ananEmail author
  • Jurairat Nunthanid
  • Sontaya Limmatvapirat
Original Article


The effects of salt forming agents for chitosan on the potential for nanoparticle formation was investigated. The salt forms were prepared from the amino acid group, including glutamic and aspartic acids, and the alpha hydroxyl acid group, including lactic and glycolic acids. All types of chitosan salt could be used to prepare bovine serum albumin (BSA) loaded nanoparticles. The chitosan salts prepared from the amino acid group showed a higher salt formation ability as demonstrated using FTIR, hence a higher %encapsulation efficiency (%EE) and a reduction in zeta potential were obtained. The difference was due to the different organic acids used giving different polymer conformations and pH values in solution. Chitosan glutamate gave the highest salt formation ability and hence the highest %EE was obtained. The release of protein from all types of chitosan was similar and chitosan glutamate exhibited the highest release. Chitosan salt is therefore a material of choice for protein-loaded nanoparticles and the characteristics of nanoparticles can be readily modulated by different types of salt form.


Chitosan salt Chitosan nanoparticle Protein Amino acid and alpha-hydroxyl acid 



This work was supported by the Research and Development Institute of Silpakorn University. This project would have been impossible without the facilities provided by the Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.


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Copyright information

© The Korean Society of Pharmaceutical Sciences and Technology 2017

Authors and Affiliations

  • Manee Luangtana-anan
    • 1
    • 2
    Email author
  • Jurairat Nunthanid
    • 1
    • 2
  • Sontaya Limmatvapirat
    • 1
    • 2
  1. 1.Department of Pharmaceutical Technology, Faculty of PharmacySilpakorn UniversityNakhon PathomThailand
  2. 2.Pharmaceutical Biopolymer Group (PBiG), Faculty of PharmacySilpakorn UniversityNakhon PathomThailand

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