Characterization of Pectin Extracted from Orange Peel Powder using Microwave-Assisted and Acid Extraction Methods

  • Anil B. Kute
  • Debabandya MohapatraEmail author
  • Nachiket Kotwaliwale
  • Saroj Kumar Giri
  • B. P. Sawant
Full-Length Research Article


Pectin extracted from dried orange peel powder by microwave extraction method (MAE) and conventional acid extraction method (CE) were compared based on their yield, color, solubility in cold and hot water, equivalent weight, degree of esterification (DE), methoxyl percentage, and gelling properties. The raw orange peel powder of 98.95 µm size had moisture 8.33%, carbohydrate 80.06%, crude protein 5.6%, fat 2.3%, ash 3.62%, L* value 66.26 ± 0.05, a* value 11.89 ± 0.02, and b* value 60.56 ± 0.15, and poor flowability. The pectin yield in MAE (15.79%) was significantly higher than CE (8.78%); time taken for processing operation was 90 s and 15 min in MAE and CE, respectively. The equivalent weight of MAE and CE pectin were 485 mg/mL and 381 mg/mL, respectively. DE and methoxyl content of MAE and CE pectin were 42.85%, 35.48%, and 6.99% and 5.75%, respectively. The CIE L*, a* and b*, values of the MAE and CE pectin powders were 41.27, 7.11, and 31.41, and 37.54, 10.96, 24.34, respectively. Complex viscosity, storage modulus, and loss modulus of CE pectin was higher than that of MAE but were not significantly different (p > 0.05). The pectin gel obtained through both the methods showed a good stability.


Orange peel Pectin Microwave extraction Conventional extraction Gelling property 



The authors are grateful to Dr. K. K. Singh, Director, ICAR—Central Institute of Agricultural Engineering, Dr. Punit Chandra, Dr. Sumedha S. Deshpande, and Dr. Dipika Agrahar-Murugkar for providing laboratory facilities. The authors also wish to acknowledge the help and support received from the Dean of College of Agricultural Engineering & Technology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbani.


  1. 1.
    Akhtar N, Uddin M (1971) Fractionation of pectins. Pak J Sci Ind Res 14(4/5):346–349Google Scholar
  2. 2.
    AOAC (ed) (1995) Official methods of analysis. Association of official analytical chemists, 12th edn. AOAC, Washington D.CGoogle Scholar
  3. 3.
    AOAC (ed) (2000) Official methods of analysis, 17th edn. Association of Official Analytical Chemists, Washington DC. ISBN: 0935584870Google Scholar
  4. 4.
    Bagherian H, Ashtiani FZ, Fouladitajar A, Mohtashamy M (2011) Comparisons between conventional, microwave-and ultrasound-assisted methods for extraction of pectin from grapefruit. Chem Eng Process Process Intensif 50(11):1237–1243Google Scholar
  5. 5.
    Carr RL (1965) Evaluating flow properties of solids. Chem Eng 72:163–168Google Scholar
  6. 6.
    Donaghy JA, McKay AM (1994) Pectin extraction from citrus peel by polygalacturonase produced on whey. Bioresource Tech 47(1):25–28Google Scholar
  7. 7.
    FAO (Food and agricultural organization) (2015). Accessed 10 June 2015
  8. 8.
    Fishman ML, Chau HK, Hoagl PD, Hotchkiss AT (2006) Microwave-assisted extraction of lime pectin. Food Hydrocoll 20(8):1170–1177Google Scholar
  9. 9.
    Garau MC, Simal S, Rossell C, Femenia A (2007) Effect of air-drying temperature on physico-chemical properties of dietary fibre and antioxidant capacity of orange (Citrus aurantium v. Canoneta) by-products. Food Chem 104(3):1014–1024Google Scholar
  10. 10.
    Grey RO, Beddow JK (1969) On the Hausner ratio and its relationship to some properties of metal powders. Powder Tech 2(6):323–326Google Scholar
  11. 11.
    Guo X, Han D, Xi H, Rao L, Liao X, Hu X, Wu J (2012) Extraction of pectin from navel orange peel assisted by ultra-high pressure, microwave or traditional heating: a comparison. Carbohydr Poly 88(2):441–448Google Scholar
  12. 12.
    Hosni K, Zahed N, Chrif R, Abid I, Medfei W, Kallel M, BenBrahim N, Sebei H (2010) Composition of peel essential oils from four selected Tunisian Citrus species: evidence for the genotypic influence. Food Chem 123(4):1098–1104Google Scholar
  13. 13.
    Hausner HH (1967) Friction conditions in a mass of metal powder. Int J Powder Metall 3:7–13Google Scholar
  14. 14.
    Hsu CP, Deshpande SN, Desrosier NW (1965) Role of pectin methylesterase in firmness of canned tomatoes. J Food Sci 30(4):583–588Google Scholar
  15. 15.
    Iglesias MT, Lozano JE (2004) Extraction and characterization of sunflower pectin. J Food Eng 62(3):215–223Google Scholar
  16. 16.
    Kanmani P, Dhivya E, Aravind J, Kumaresan K (2014) Extraction and analysis of pectin from citrus peels: augmenting the yield from citrus lemon using statistical experimental design. Iran J Energy Environ 5(3):303–312Google Scholar
  17. 17.
    Kar F, Arslan N (1999) Effect of temperature and concentration on viscosity of orange peel pectin solutions and intrinsic viscosity–molecular weight relationship. Carbohydr Poly 40(4):277–284Google Scholar
  18. 18.
    Kim WC, Lee DY, Lee CH, Kim CW (2004) Optimization of narirutin extraction during washing step of the pectin production from citrus peels. J Food Eng 63(2):191–197Google Scholar
  19. 19.
    Kratchanova M, Panchev I, Pavlova E, Shtereva L (1994) Extraction of pectin from fruit materials pretreated in an electromagnetic field of super-high frequency. Carbohydr Poly 25(3):141–144Google Scholar
  20. 20.
    Kratchanova M, Pavlova E, Panchev I (2004) The effect of microwave heating of fresh orange peels on the fruit tissue and quality of extracted pectin. Carbohydr Poly 56:181–185Google Scholar
  21. 21.
    Kulkarni SG, Vijayanand P (2010) Effect of extraction conditions on the quality characteristics of pectin from passion fruit peel (Passiflora edulis f. flavicarpa L.). LWT-Food Sci Tech 43(7):1026–1031Google Scholar
  22. 22.
    Kumar P, Mishra S, Malik A, Satya S (2012) Insecticidal evaluation of essential oils of Citrus sinensis L. (Myrtales: Myrtaceae) against housefly, Musca domestica L. (Diptera: Muscidae). Parasitol Res 110(5):1929–1936Google Scholar
  23. 23.
    Kute A, Mohapatra D, Babu VB, Sawant BP (2015) Optimization of microwave assisted extraction of pectin from orange peel using response surface methodology. J Food Res Tech 3(2):62–70Google Scholar
  24. 24.
    Li D, Jia X, Wei Z, Liu Z (2012) Box-behnken experimental design for investigation of microwave-assisted extracted sugar beet pulp pectin. Carbohydr Poly 88(1):342–346Google Scholar
  25. 25.
    Liew SQ, Chin NL, Yusof YA (2014) Extraction and characterization of pectin from passion fruit peels ST26943. In: 2nd International conference on agricultural & food engineering, CAFEi2014” agriculture & agricultural science procedia, vol 2, pp. 231–236Google Scholar
  26. 26.
    Liu Y, Shi J, Langrish TAG (2006) Water-based extraction of pectin from flavedo & albedo of orange peels. Chem Eng J 120(3):203–209Google Scholar
  27. 27.
    Maran JP, Sivakumar V, Thirugnanasambham K, Sridhar R (2013) Optimization of microwave assisted extraction of pectin from orange peel. Carbohydr Poly 97(2):703–709Google Scholar
  28. 28.
    Min B, Lim J, Sanghoon K, Kwang GL, Sung HL, Suyong L (2011) Environmentally friendly preparation of pectins from agricultural byproducts and their structural/rheological characterization. Bioresource Tech 102:3855–3860Google Scholar
  29. 29.
    Morris GA, Foster TJ, Harding SE (2000) The effect of the degree of esterification on the hydrodynamic properties of citrus pectin. Food Hydrocoll 14(3):227–235Google Scholar
  30. 30.
    Rezzadori K, Benedetti S, Amante ER (2012) Proposals for the residues recovery: orange waste as raw material for new products. Food Biol Prod Process 90:606–614Google Scholar
  31. 31.
    Schemin MHC, Fertonani HCR, Waszczynskyj N, Wosiacki G (2005) Extraction of pectin from apple pomace. Brazil Arch Biol Tech 48:259–266Google Scholar
  32. 32.
    Shkodina OG, Zeltser OA, Selivanov NY, Ignatov VV (1998) Enzymic extraction of pectin preparations from pumpkin. Food Hydrocoll 12(3):313–316Google Scholar
  33. 33.
    Singthong J, Ningsanond S, Cui SW, Goff HD (2005) Extraction & physicochemical characterization of Krueo Ma Noy pectin. Food Hydrocoll 19(5):793–801Google Scholar
  34. 34.
    Srivastava P, Malviya R (2011) Extraction, characterization and evaluation of orange peel waste derived pectin as a pharmaceutical excipient. Nat Prod J 1(1):65–70Google Scholar
  35. 35.
    Subba MS, Soumithri TC, Rao RS (1967) Antimicrobial action of citrus oils. J Food Sci 32(2):225–227Google Scholar
  36. 36.
    Tabilo-Munizaga G, Barbosa-Canovas GV (2005) Rheology for the food industry. J Food Eng 67:147–156Google Scholar
  37. 37.
    Wang S, Chen F, Wu J, Wang Z, Liao X, Hu X (2007) Optimization of pectin extraction assisted by microwave from apple pomace using response surface methodology. J Food Eng 78(2):693–700Google Scholar
  38. 38.
    Yeoh S, Shi J, Langrish TAG (2008) Comparisons between different techniques for water-based extraction of pectin from orange peels. Desalination 218(1):229–237Google Scholar
  39. 39.
    Zouambia YK, Youcef E, Mohamed K, Nadji MM (2014) A new approach for pectin extraction: electromagnetic induction heating. Arab J Chem 10:480. Google Scholar

Copyright information

© NAAS (National Academy of Agricultural Sciences) 2019

Authors and Affiliations

  1. 1.College of Agricultural Engineering and TechnologyVasantrao Naik Marathwada Krishi VidyapeethParbhaniIndia
  2. 2.Agro-produce Processing DivisionICAR-Central Institute of Agricultural EngineeringBhopalIndia

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