Antioxidant and Antimicrobial Efficacy of Peptidic Hydrolysate Obtained from Porcine Blood
Aim of present research was to explore changes in pH, degree of hydrolysis (DH) antioxidant [2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 2,2′-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing-antioxidant power assay (FRAP)] and antibacterial property of enzymatic hydrolysates obtained from hydrolysis of porcine blood using proteases viz., alcalase, trypsin and papain. It was observed that pH of porcine blood hydrolysates slightly decreased as hydrolysis progressed for all the enzymes. DH improved considerably (p < 0.05) up to 6 h for alcalase and papain digested samples, while it increased significantly (p < 0.05) up to 4 h for trypsin. ABTS, DPPH, FRAP assay and antimicrobial activity improved appreciably (p < 0.05) indicating positive correlation with duration of digestion and DH. SDS-PAGE showed distinct decrease in amount and intensity of protein bands with progressing duration of digestion. It can be concluded from the study that porcine blood can be utilized as excellent substrate for production of protein hydrolysates with potent antioxidant and antimicrobial activities which may be employed for meat/food products preservation.
KeywordsAntimicrobial Antioxidant Degree of hydrolysis Enzymatic hydrolysis Hydrolysates
First author is thankful to Department of Science and Technology, Ministry of Science and Technology, Government of India for financial assistance provided in the form of Inspire Fellowship (JRF-P).
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interests.
- 1.Adler-Nissen J (1986) Enzymic hydrolysis of food proteins. Elsevier, New YorkGoogle Scholar
- 3.AOAC (2000) Official methods of analysis, 17th edn. Association of Official Analytical Chemists, WashingtonGoogle Scholar
- 4.Balaswamy K, PrabhakaraRao PG, Narsing RG, Jyothirmayi T (2011) Functional properties of roe protein hydrolysates from Catla Catla. Electron J Environ Agric Food Chem 10:2139–2147Google Scholar
- 12.Hiidenhovi J, Hietanen A, Makinen J, Huopalahti R, Ryhanen EL (2005) Hydrolysis of ovomucin by different enzymes. In: XVIIth European symposium on the quality of eggs and egg products, Doorwerth. CAB Direct, Wallingford, pp 251–256Google Scholar
- 26.Salwanee SWM, Wan A, Mamot S, Maskat MY, Ibrahim S (2013) Effects of enzyme concentration, temperature, pH and time on the degree of hydrolysis of protein extract from viscera of tuna (Euthynnus affinis) by using alcalase. Sains Malaysian 42:279–287Google Scholar
- 28.Verma AK, Chatli MK, Kumar P, Mehta N (2017) Antioxidant and antimicrobial activity of protein hydrolysate extracted from porcine liver. Indian J Anim Sci 87:711–717Google Scholar