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Local bivalve allergens — a Malaysian perspective

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Abstract

Background

Apart from the nutritional benefits, bivalves are also considered to be among a group of allergenic foods. Symptoms of allergy to bivalves are largely similar to those of allergic reactions reported for other shellfish. The identification of allergenic proteins in a particular species is an important step for the development of more accurate allergy tests and for the definition of more effective management of patients. Currently, studies regarding the allergens in bivalve species are lacking.

Materials and methods

This minireview is an overview of local scientific articles on identification of bivalve allergens by allergenomics methods including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE), immunoblotting and mass-spectrometry analysis.

Results

Tropomyosin has been the only well recognized allergen in a wide range of bivalve species. In addition to tropomyosin, our local study resulted in discovery of three novel bivalve major allergens; arginine kinase (40 kDa), actin (42 kDa) and troponin C (18 kDa).

Conclusion

This minireview will provide a better understanding of each allergen derived from local bivalves which subsequently will help in the understanding of the specific immunoglobulin E(IgE)-mediated immune response.

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Abbreviations

2-DE:

Two dimensional-gel electrophoresis

cDNA:

Complementary deoxyribonucleic acid

ELISA:

Enzyme-linked immunosorbent assay

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide-gel electrophoresis

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Author information

Authors and Affiliations

  1. Allergy and Immunology Research Centre, Institute for Medical Research, Jalan Pahang, 50588, Kuala Lumpur, Malaysia

    Zailatul Hani Mohamad Yadzir, Faizal Bakhtiar & Noormalin Abdullah

  2. Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Tanjong Malim, Malaysia

    Misnan Rosmilah & Syakirah Samsudin

Authors
  1. Zailatul Hani Mohamad Yadzir
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  2. Misnan Rosmilah
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  3. Faizal Bakhtiar
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  4. Syakirah Samsudin
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  5. Noormalin Abdullah
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Corresponding author

Correspondence to Zailatul Hani Mohamad Yadzir.

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Acknowledgements

The authors thank the Director General of Health Malaysia for permission to publish this paper.

Author’s contributions

Z. H. M. Yadzir: interpretation of results and analysis, writing the paper. R. Misnan, F. Bakhtiar, S. Samsudin, N. Abdullah: interpretation of results and analysis, critical revision of the paper.

Conflict of interest

Z.H.M. Yadzir, R. Misnan, F. Bakhtiar, S. Samsudin and N. Abdullah declare that they have no competing interests.

Cite this as

Yadzir ZHM, Rosmilah M, Bakhtiar F, Samsudin S, Abdullah N. Local bivalve allergens — a Malaysian perspective. Allergo J Int 2018;27:228–33 https://doi.org/10.1007/s40629-018-0057-z

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Yadzir, Z.H.M., Rosmilah, M., Bakhtiar, F. et al. Local bivalve allergens — a Malaysian perspective. Allergo J 27, 32–37 (2018). https://doi.org/10.1007/s15007-018-1721-9

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  • DOI: https://doi.org/10.1007/s15007-018-1721-9

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