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Tissue Engineering and Regenerative Medicine

, Volume 16, Issue 2, pp 201–212 | Cite as

Stem Cells Seeded on Multilayered Scaffolds Implanted into an Injured Bladder Rat Model Improves Bladder Function

  • Kshitiz Raj Shrestha
  • Seung Hwan Jeon
  • Ae Ryang Jung
  • In Gul Kim
  • Ga Eun Kim
  • Yong Hyun Park
  • Soo Hyun Kim
  • Ji Youl LeeEmail author
Original Article
  • 116 Downloads

Abstract

Background:

To investigate whether human adipose-derived stem cells (hADSCs) seeded on multilayered poly (l-lactide-co-ɛ-caprolactone) (PLCL) sheets improve bladder function in a rat model of detrusor smooth muscle-removed bladder.

Methods:

Male rats were randomly divided into 4 groups: Normal, injury (detrusor smooth muscle-removed bladder), PLCL (detrusor smooth muscle-removed bladder implanted with PLCL sheets), and PLCL + ADSC (detrusor smooth muscle-removed bladder implanted with PLCL sheets seeded with hADSCs). Four weeks after the treatment, physiological, histological, immunohistochemical, and immunoblot analyses were performed.

Results:

hADSCs were compatible with PLCL sheets. Further, the physiological study of PLCL + ADSC group showed significant improvement in compliance and contractility suggesting the functional improvement of the bladder. Histological, immunohistochemical and immunoblot analyses revealed the uniform distribution of hADSCs in between PLCL sheets as well as differentiation of hADSCs into smooth muscle cells (SMC) which is illustrated by the expression of SMC markers.

Conclusion:

hADSCs seeded on the multilayered PLCL sheets has the potential to differentiate into SMC, thus facilitating the recovery of compliance and contractility of the injured bladder.

Keywords

Urinary bladder Smooth muscle Tissue engineering Stem cells Compliance 

Notes

Acknowledgement

This research was supported by the National Research Foundation of Korea grant funded by the Korea government (MSIP) (No. 2011-0030075).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

The animal studies were performed after receiving approval of the Institutional Animal Care and Use Committee (IACUC) in The Catholic University of Korea, Seoul, Korea (IACUC Approval No. 2013-0111-03).

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Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Nature B.V. 2019

Authors and Affiliations

  • Kshitiz Raj Shrestha
    • 1
    • 2
  • Seung Hwan Jeon
    • 1
  • Ae Ryang Jung
    • 1
    • 2
  • In Gul Kim
    • 1
    • 2
  • Ga Eun Kim
    • 1
    • 2
  • Yong Hyun Park
    • 1
    • 2
  • Soo Hyun Kim
    • 3
  • Ji Youl Lee
    • 1
    • 2
    • 4
    Email author
  1. 1.Department of Urology, College of Medicine, Seoul St. Mary’s HospitalThe Catholic University of KoreaSeoulRepublic of Korea
  2. 2.Cancer Research InstituteThe Catholic University of KoreaSeoulRepublic of Korea
  3. 3.Center for Biomaterials, Biomedical Research InstituteKorea Institute of Science and Technology (KIST)SeoulRepublic of Korea
  4. 4.Department of BioinformaticsThe Catholic University of KoreaSeoulRepublic of Korea

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