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Tissue Engineering and Regenerative Medicine

, Volume 16, Issue 2, pp 161–175 | Cite as

Differentiation Capacity of Monocyte-Derived Multipotential Cells on Nanocomposite Poly(e-caprolactone)-Based Thin Films

  • Iro Koliakou
  • Eleni GounariEmail author
  • Maria NerantzakiEmail author
  • Eleni Pavlidou
  • Dimitrios Bikiaris
  • Martha Kaloyianni
  • George Koliakos
Original Article

Abstract

Background:

Μonocyte-derived multipotential cells (MOMCs) include progenitors capable of differentiation into multiple cell lineages and thus represent an ideal autologous transplantable cell source for regenerative medicine. In this study, we cultured MOMCs, generated from mononuclear cells of peripheral blood, on the surface of nanocomposite thin films.

Methods:

For this purpose, nanocomposite Poly(e-caprolactone) (PCL)-based thin films containing either 2.5 wt% silica nanotubes (SiO2ntbs) or strontium hydroxyapatite nanorods (SrHAnrds), were prepared using the spin-coating method. The induced differentiation capacity of MOMCs, towards bone and endothelium, was estimated using flow cytometry, real-time polymerase chain reaction, scanning electron microscopy and fluorescence microscopy after cells’ genetic modification using the Sleeping Beauty Transposon System aiming their observation onto the scaffolds. Moreover, Wharton’s Jelly Mesenchymal Stromal Cells were cultivated as a control cell line, while Human Umbilical Vein Endothelial Cells were used to strengthen and accelerate the differentiation procedure in semi-permeable culture systems. Finally, the cytotoxicity of the studied materials was checked with MTT assay.

Results:

The highest differentiation capacity of MOMCs was observed on PCL/SiO2ntbs 2.5 wt% nanocomposite film, as they progressively lost their native markers and gained endothelial lineage, in both protein and transcriptional level. In addition, the presence of SrHAnrds in the PCL matrix triggered processes related to osteoblast bone formation.

Conclusion:

To conclude, the differentiation of MOMCs was selectively guided by incorporating SiO2ntbs or SrHAnrds into a polymeric matrix, for the first time.

Graphical Abstract

Keywords

Monocyte-derived multipotential cells Poly(ε-caprolactone) Silica nanotubes Strontium hydroxyapatite nanorods 

Notes

Acknowledgements

The authors thank Biohellenika SA Biotechnology Company for providing the facilities and consumables that enabled the completion of this study. EG and GK conceived the study; MN and DB fabricated and characterized the materials; IK and EG designed and performed the experiments (equal contribution to this work); IK, EG, EP, MK and DB analysed the data; IK, EG and MN wrote the manuscript. All authors reviewed the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This study was approved by the Ethics Committee of Aristotle University of Thessaloniki, School of Medicine (390-9/1.7.2017) and University Hospital AXEPA of Thessaloniki (3868/24.1.2018). This research involves Human Participants after their informed consent. Peripheral blood samples were collected from healthy volunteer donors while mesenchymal stromal cells and HUVEC were isolated from Wharton’s jelly after parents approval during stem cell banking in Biohellenika SA Biotechnology Company.

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Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Nature B.V. 2019

Authors and Affiliations

  • Iro Koliakou
    • 1
    • 2
  • Eleni Gounari
    • 2
    • 3
    Email author
  • Maria Nerantzaki
    • 4
    • 5
    Email author
  • Eleni Pavlidou
    • 6
  • Dimitrios Bikiaris
    • 4
  • Martha Kaloyianni
    • 1
  • George Koliakos
    • 2
    • 3
  1. 1.Department of Biology, Laboratory of Animal PhysiologyAristotle University of ThessalonikiThessaloníkiGreece
  2. 2.Biohellenika Biotechnology CompanyThessaloníkiGreece
  3. 3.Department of Biochemistry, Medical School, Faculty of Health SciencesAristotle University of ThessalonikiThessaloníkiGreece
  4. 4.Department of Chemistry, Laboratory of Polymer Chemistry and TechnologyAristotle University of ThessalonikiThessaloníkiGreece
  5. 5.PHysico-Chimie des Electrolytes et Nanosystèmes InterfaciauX (PHENIX)Sorbonne UniversitéParisFrance
  6. 6.Department of PhysicsAristotle University of ThessalonikiThessaloníkiGreece

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