Tissue Engineering and Regenerative Medicine

, Volume 16, Issue 2, pp 191–200 | Cite as

Treatment of Periodontal Bone Defects with Stem Cells from Inflammatory Dental Pulp Tissues in Miniature Swine

  • Ye Li
  • Xi Nan
  • Tian-Yu Zhong
  • Tong LiEmail author
  • Ang Li
Original Article



Containing a certain proportion of mesenchymal stem cells, inflammatory dental tissue showed great tissue regeneration potential in recent years. However, whether it is applicable to promote tissue regeneration in vivo remains to be elucidated. Therefore, we evaluated the feasibility of stem cells from inflammatory dental pulp tissues (DPSCs-IPs) to reconstruct periodontal defects in miniature pigs.


The autologous pig DPSCs-IPs were first cultured, appraised and loaded onto β-tricalcium phosphate (β-TCP). The compounds were then engrafted into an artificially-created periodontal defect. Three months later, the extent of periodontal regeneration was evaluated. Clinical examination, radiological examination and immunohistochemical staining were used to assess periodontal regeneration.


The data collectively showed that DPSCs-IPs from miniature pigs expressed moderate to high levels of STRO-1 and CD146 as well as low levels of CD34 and CD45. DPSCs-IPs have osteogentic, adipogenic and chondrogenic differentiation abilities. DPSCs-IPs were engrafted onto β-TCP and regenerated bone to repair periodontal defects by 3 months’ post-surgical reconstruction.


Autologous DPSCs-IPs may be a feasible means of periodontal regeneration in miniature pigs.


Dental pulp tissue Bone defeats Stem cells Periodontitis 



This research was funded by Shaanxi Provincial Science and Technology Innovation Project co-ordination of Resources Oriented Industries of Key Technologies Project (Grant No. 2011KTCL03-24 for A.L.) and Scientific and Technological Innovation Project in Xi’an (Grant No. SF1421 for T.L.).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

The study has been independently reviewed and approved by the ethical board of Xi’an Jiaotong University College of Medicine for Experimental Animals (No. 2017036).


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Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Nature B.V. 2019

Authors and Affiliations

  • Ye Li
    • 1
    • 2
  • Xi Nan
    • 2
  • Tian-Yu Zhong
    • 3
  • Tong Li
    • 1
    • 3
    Email author
  • Ang Li
    • 1
    • 2
  1. 1.Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of StomatologyXi’an Jiaotong UniversityXi’anChina
  2. 2.Department of Periodontology, College of StomatologyXi’an Jiaotong UniversityXi’anChina
  3. 3.Department of Orthodontics, College of StomatologyXi’an Jiaotong UniversityXi’anChina

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