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Tissue Regeneration of Human Mesenchymal Stem Cells on Porous Gelatin Micro-Carriers by Long-Term Dynamic In Vitro Culture

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Tissue engineering is a multidisciplinary field which attracted much attention in recent years. One of the most important issue in tissue engineering is how to obtain high cell numbers and tissue regeneration while maintaining appropriate cellular characteristics in vitro for restoring damaged or dysfunctional body tissues and organs. These demands can be achieved by the use of three dimensional (3D) dynamic cultures of cells combined with cell-adhesive micro-carriers.

Method:

In this study, human mesenchymal stem cells (hMSCs) were cultured in a wave-bioreactor system for up to 100 days, after seeding on Cultisphere-S porous gelatin micro-carriers. Cell counting was performed at the time points of 7, 12, 17, 31 days and compared to those of hMSCs cultured under static condition. Higher growth and proliferation rates was achieved in wave-type dynamic culture, when cell culture continued to day 31. A scanning electron microscope (SEM) photographs, both live and dead and MTT assays were taken to confirm the survival and distribution of cells on porous gelatin micro-carrier surfaces. The results of histological stains such as hematoxylin and eosin, Masson’s trichrome, Alcian blue and Alizarin red S also showed improved proliferation and tissue regeneration of hMSCs on porous gelatin micro-carriers.

Conclusion:

The experimental results demonstrated the effect and importance of both micro-carriers and bioreactor in hMSC expansion on cell proliferation and migration as well as extracellular matrix formation on the superficial and pore surfaces of the porous gelatin micro-carriers, and then their inter-connections, leading to tissue regeneration.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) Grant (2015R1A2A1A10054592). Human mesenchymal stem cells has been donated by Professor Sungwon Kim in the Catholic University of Korea St Mary’s Hospital, Seoul, Korea.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul, 11811, Republic of Korea

    LeTuyen Nguyen & Insup Noh

  2. Convergence Institute of Biomedical Engineering and Biomaterials, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul, 11811, Republic of Korea

    Sumi Bang & Insup Noh

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  1. LeTuyen Nguyen
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  2. Sumi Bang
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Correspondence to Insup Noh.

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Nguyen, L., Bang, S. & Noh, I. Tissue Regeneration of Human Mesenchymal Stem Cells on Porous Gelatin Micro-Carriers by Long-Term Dynamic In Vitro Culture. Tissue Eng Regen Med 16, 19–28 (2019). https://doi.org/10.1007/s13770-018-00174-8

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  • DOI: https://doi.org/10.1007/s13770-018-00174-8

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