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Biodegradation of monocrotophos by a plant growth promoting Bacillus aryabhattai (VITNNDJ5) strain in artificially contaminated soil

  • D. M. Dash
  • J. W. OsborneEmail author
Original Paper

Abstract

The present study was focused on the biodegradation of monocrotophos (MCP) by indigenous bacteria isolated from sugarcane cultivable soil. The isolates were tested for pesticide tolerance, and the strain VITNNDJ5 showed maximum MCP tolerance and highest degradation potential. The presence of candidate gene for pesticide degradation and plant growth promoting rhizobacterial traits confirmed VITNNDJ5 to be the effective strain. Biodegradation of MCP (1000 mg L−1) was monitored using UV spectrophotometer and HPLC; the degradation products were identified by GCMS. The degradation kinetics was analyzed, and the rate constant (k) and half-life (t1/2) were calculated. Maximum degradation of up to 93% was observed within 5 days. The 16S rRNA gene sequencing revealed VITNNDJ5 to be the closest neighbor of Bacillus aryabhattai (GenBank accession number—KU598848). The augmentation of VITNNDJ5 to the rhizosphere of Liriope muscari enhanced the plant growth and degradation of MCP in soil. The fate of MCP in soil was analyzed, and a metabolic pathway was proposed.

Keywords

Organophosphorus Rhizobacteria Phytoremediation Rhizoremediation Degradation kinetics 

Notes

Acknowledgements

The authors would like to thank the management of Vellore Institute of Technology for providing all the resources and technical support aided to carry out the research. Heartfelt thanks to Dr. S. Vino and Dr. S. Sajitha Lulu for providing help for in silico studies. Authors extend their gratitude to DST–FIST cum VIT Funded Scanning Electron Microscope Lab Facility, SIF-VIT for GC–MS and FTIR facilities and TBI–VIT for providing and HPLC facility to carry out analytical studies.

Compliance with ethical standards

Conflict of interest

The authors would like to declare that they have no conflict of interest.

Supplementary material

13762_2019_2432_MOESM1_ESM.docx (852 kb)
Supplementary material 1 (DOCX 848 kb)

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© Islamic Azad University (IAU) 2019

Authors and Affiliations

  1. 1.Department of Biosciences, School of Bio Sciences and TechnologyVellore Institute of TechnologyVelloreIndia

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