Domestic wastewater hydrolysis and lipolysis during start-up in anaerobic digesters and microbial fuel cells at moderate temperatures
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Raw wastewater hydrolysis rates during start-up in microbial fuel cells (MFCs) and anaerobic digestion (AD) systems, seeded with a mesophilic inoculum from a digester, were compared at moderate temperatures (27.5 ℃ and 8 ℃). Temperature drop affected both the lipids and carbohydrates hydrolysis rates but not necessarily the protein removal rates (temperature-independent rates of MFC), which were significantly influenced from treatment alteration (AD to MFC). MFC showed robust proteolysis at low temperature compared to AD; the latter seems to have a higher potential at warmer conditions. A lipases activity assay showed that although at 27.5 ℃ both AD and MFC are likely to hydrolyse lipids, the latter has a higher lipolysis potential at low temperatures. Preliminary community structure analysis showed that the switch from AD to MFC alters the bacterial community by 15% with the MFC showing higher diversification; temperature decrease, though, alters the community by 40%. Key organisms that appear to be favoured at the MFC set-ups are Geobacteriaceae, taxa likely related to the hydrolytic capacity of this set-up.
KeywordsCold-adapted Hydrolysis Lipolysis Low-temperature wastewater treatment Microbial fuel cells
This work funded by the Engineering and Physical Sciences Research Council, UK (Grant reference EP/G032033/1). The authors would also like to thank Mr. Kangxu Wang for his assistance with the chemical–molecular tests and Dr. Jan Dolfing for reviewing the manuscript.
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Conflict of interest
We declare that there is no conflict of interest.
- APHA (1995) WPCF, standard methods for the examination of water and wastewater. American Public Health Association/American Water Works Association/Water Environment Federation, Washington DCGoogle Scholar
- Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL (2008) GenBank. Nucleic Acids Res 36(Database issue):D25–30Google Scholar
- Dolfing J, Janssen DB (1994) Estimates of Gibbs free energies of formation of chlorinated aliphatic compounds. Biodegradation 5(1):21–28Google Scholar
- Hedje JE, Hofreiter BT (1962) In: Whistler RL, Be Miller JN (eds) Carbohydrates chemistry, 17th edn. Academic Press, New York.Google Scholar
- Heidrich, E.S. (2012) Evaluation of microbial electrolysis cells in the treatment of domestic wastewater, Ph.D. Thesis, E-Theses Newcastle University, UKGoogle Scholar
- Lawrence AW, McCarty PL (1969) Kinetics of methane fermentation in anaerobic treatment. Journal (Water Pollution Control Federation) 41(2):R1–17Google Scholar
- Logan BE (2008) Microbial fuel cells. John Wiley & Sons, LondonGoogle Scholar
- Malina J, Pohland FG (1992) Design of anaerobic processes for treatment of industrial and municipal waste, vol VII. Routledge, LondonGoogle Scholar
- Mobarak-Qamsari E, Kasra-Kermanshahi R, Nosrati M, Amani T (2012) Enzymatic pre-hydrolysis of high fat content dairy wastewater as a pretreatment for anaerobic digestion. Int J Environmental Res 6(2):475–480Google Scholar
- Petropoulos E, Yu Y, Tabraiz S, Yakubu A, Curtis T, Dolfing J (2019) High rate domestic wastewater treatment at 15 ℃ using anaerobic reactors inoculated with cold-adapted sediments/soils – shaping robust methanogenic communities. Environ Sci Water Res Technol. https://doi.org/10.1039/C8EW00410B CrossRefGoogle Scholar
- Rifkin J (2002) The hydrogen economy: the creation of the worldwide energy web and the redistribution of power on earth. Tarcher/Putnam, New YorkGoogle Scholar
- Sanders S, Bergen D, Buijs S, Corstanje R, Gerrits M, Hoogerwerf T, Kanwar S, Zeeman G, Groenestijn J, Lettinga G (1996) Treatment of waste activated sludge in an anaerobic hydrolysis upflow sludge bed reactor. In: Proceedings of 10th EWPCA-symposium on sewage and refuse, liquid wastes section, München. GFA, Hennef, pp 277–305Google Scholar
- Van Haandel AC, Lettinga G (1994) Anaerobic sewage treatment: a practical guide for regions with a hot climate. John Wiley & Sons, LondonGoogle Scholar