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Reinforcement of methanogenesis in anaerobic digesters through the application of a purple non-sulfur bacteria bio-augmentation scheme

  • M. S. HammamEmail author
  • K. Z. Abdalla
Original Paper
  • 9 Downloads

Abstract

Purple non-sulfur bacteria generally possess a distinguishingly diverse metabolism, rendering them being classified among the most adaptable bacteria. Rhodobacter capsulatus, ATCC® 11166™ strain, was utilized for inoculating two bench-scale anaerobic digestion reactors, within an experimental configuration comprising five bench-scale anaerobic digestion reactors. The investigation assessed the effect of applying a bio-augmentation scheme onto anaerobic digestion processes, with respect to biodegradation, resilience, and energy capture efficiency. The results implied significant evidence of the positive impact of the applied scheme onto the methanogenesis process within the bio-augmented reactors, comparing the outputs attained within the bio-augmented reactors to the non-bio-augmented reactors. An increase within the specific methane production per added and destroyed chemical oxygen demand was observed, associated with a consequent increase in chemical oxygen demand destruction. This indicated an overall enhancement of the anaerobic digestion processes, specifically those typically attributed to the methane-forming Archaea. The bio-augmented reactors were also subjected to a set of perturbation-causing conditions, aiming to assess the performance of the methanogenic culture under such conditions, as compared to non-bio-augmented reactors that are subjected to the same conditions. A relatively higher and more stable methane production rate was observed within the bio-augmented reactors, during the application of the referred-to conditions.

Keywords

Rhodobacter capsulatus Biogas Organic load assimilation capacity Illumination Paired reactors 

Notes

Acknowledgements

This study is part of a research project, aimed for partial fulfillment of a Doctor of Philosophy Thesis, submitted for the “Department of Public Works” at the “Faculty of Engineering—Cairo University”. The authors would like to thank the technical team at the “Sanitary and Environmental Engineering Laboratory” at the “Cairo University—Faculty of Engineering”, for their support that helped achieve the experimental work throughout the study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Akkerman I, Janssen M, Rocha J, Wijffels RH (2002) Photobiological hydrogen production: photochemical efficiency and bioreactor design. Int J Hydrogen Energy 27:1195–1208CrossRefGoogle Scholar
  2. Androga DD, Özgür E, Eroglu I, Gündüz U, Yücel M (2012) Photofermentative hydrogen production in outdoor conditions. In: Minic D (ed) Hydrogen energy—challenges and perspectives. In Tech, Belgrade, pp 77–120Google Scholar
  3. Angelidaki I, Ellegaard L, Ahring BK (1993) A mathematical model for dynamic simulation of anaerobic digestion of complex substrates: focusing on ammonia inhibition. Biotechnol Bioengng 42:159–166CrossRefGoogle Scholar
  4. Basak N, Das D (2007) The prospect of purple non-sulfur (PNS) photosynthetic bacteria for hydrogen production: the present state of the art. World J Microbiol Biotechnol 23:31–42CrossRefGoogle Scholar
  5. Brune DC (1995) Sulfur compounds as photosynthetic electron donors. In: Blankenship RE, Madigan MT, Bauer CE (eds) Anoxygenic photosynthetic Bacteria, vol 2. Advances in photosynthesis and respiration. Springer, Dordrecht, pp 847–870.  https://doi.org/10.1007/0-306-47954-0_39 CrossRefGoogle Scholar
  6. Costa S, Ganzerli S, Rugiero I, Pellizzari S, Pedrini P (2017) Tamburini E (2017) Potential of Rhodobactercapsulatus grown in anaerobic-light or aerobic-dark conditions as bioremediation agent for biological wastewater treatments. Water 9:108.  https://doi.org/10.3390/w9020108 CrossRefGoogle Scholar
  7. Drosg B (2013) Process monitoring in biogas plant, IEA Bioenergy, IEA Bioenergy Electronic Publications. https://www.ieabioenergy.com/publications/process-monitoring-in-biogas-plants. Accessed 22 Apr 2017
  8. Ehrenreich A, Widdel F (1994) Anaerobic oxidation of ferrous iron by purple bacteria, a new type of phototrophic metabolism. Appl Environ Microbiol 60(12):4517–4526Google Scholar
  9. Fascetti E, D’addario E, Todini O, Robertiello A (1998) Photosynthetic hydrogen evolution with volatile organic acids derived from the fermentation of source selected municipal solid wastes. Int J Hydrogen Energy 23:753–760CrossRefGoogle Scholar
  10. Gerardi MH (2003) The microbiology of anaerobic digesters. Wiley, New JerseyCrossRefGoogle Scholar
  11. Hädicke O, Grammel H, Klamt S (2011) Metabolic network modeling of redox balancing and biohydrogen production in purple nonsulfur bacteria. BMC Syst Biol 5:150CrossRefGoogle Scholar
  12. Hansen TA, Imhoff JF (1985) Rhodobacter Veldkampii: a new species of phototrophic purple non-sulfur bacteria. Int J Syst Bacteriol 35(1):115–116CrossRefGoogle Scholar
  13. Hansen KH, Angelidaki I, Ahring BK (1998) Anaerobic digestion of swine manure: inhibition by ammonia. Water Res 32(1):5–12CrossRefGoogle Scholar
  14. Holm HW, Vennes JW (1970) Occurrence of purple sulfur bacteria in a sewage treatment lagoon. Appl Microbiol 19(6):988–996Google Scholar
  15. Imhoff JF (2006) The phototrophic alpha-proteobacteria. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The prokaryotes. Springer, New York.  https://doi.org/10.1007/0-387-30745-1_2 Google Scholar
  16. Kapp H (1984) Schlammfaulung mit hohem Feststoffgehalt. Stuttgarter Berichte zur Siedlungswasserwirtschaft, Bd. 86, Oldenbourg Verlag, MünchenGoogle Scholar
  17. Labatut RA, Gooch CA (2014) Monitoring of anaerobic digestion process to optimize performance and prevent system failure. Cornell University, IthacaGoogle Scholar
  18. Liebetrau J, Pfeiffer D, Thrän D (2012) Messmethodensammlung Biogas – Methoden zur Bestimmung von analytischen und prozessbeschreibenden Parametern im Biogasbereich. Schriftenreihe des BMU-Förderprogrammes, Energetische Biomassenutzung – Band 7, DBFZ, Leipzig, GermanyGoogle Scholar
  19. Madigan MT, Gest H (1979) Growth of the photosynthetic bacterium Rodopseudomonas capsulata chemoautotrophically in darkness with H2 as the energy source. J Bacteriol 137:524–530Google Scholar
  20. Madigan MT, Jung DO (2009) An overview of purple bacteria: systematics, physiology, and habitats. In: Hunter CN, Daldal F, Thurnauer MC, Beatty JTh (eds) The purple phototrophic bacteria. Springer, Dordrecht, pp 1–15Google Scholar
  21. Miyake J, Kawamura S (1987) Efficiency of light energy conversion to Hydrogen by photosynthetic bacteria Rhodobacter sphaeroides. Int J Hydrogen Energy 12:147–149CrossRefGoogle Scholar
  22. Nakada E, Nishikata S, Asada Y, Miyake J (1998) Light penetration and wavelength effect on photosynthetic bacteria culture for hydrogen production. In: Zaborsky OR (ed) Biohydrogen. Plenum Press, London, pp 345–352Google Scholar
  23. Nordmann W (1977) Die Überwachung der Schlammfaulung. KA-Informationen für das Betriebspersonal, Beilage zur Korrespondenz Abwasser, 3/77Google Scholar
  24. Reaume S (2009) Fermentation of glucose and xylose to Hydrogen in the presence of long chain fatty acids. University of Windsor, Ontario, Canada. ©2009 Stephen ReaumeGoogle Scholar
  25. Sander J, Dahl C (2008) Metabolism of inorganic sulfur compounds in purple bacteria. In: Hunter CN, Daldal F, Thurnauer MC, Beatty JTh (eds) The purple phototrophic bacteria. Springer, Dordrecht, pp 595–622Google Scholar
  26. Siefert E, Irgens R, Pfennig N (1978) Phototrophic purple and green bacteria in sewage treatment plant. Applied Environ. Microbiol. 35(1):38–44Google Scholar
  27. Siles JA, Brekelmans J, Martín MA, Chica AF, Martín A (2010) Impact of ammonia and sulphate concentration on thermophilic anaerobic digestion. Bioresour Technol 101:9040–9048CrossRefGoogle Scholar
  28. Sung S, Santha H (2003) Performance of temperature-phased anaerobic digestion (TPAD) system treating dairy cattle wastes. Water Res 37(7):1628–1636.  https://doi.org/10.1016/S0043-1354(02)00498-0 CrossRefGoogle Scholar
  29. Suwansaard M (2010) Production of Hydrogen and 5-Aminolevulinic acid by photosynthetic Bacteria from palm oil mill effluent. Doctoral thesis, Prince of Songkla University. ThailandGoogle Scholar
  30. Vincezini M, Materassi R, Tredici MR (1982) H2 production by immobilized cell: light dependent dissimilation of organic substance by Rhodopseudomonas palustris. Int J Hydrogen Energy 7:231–236CrossRefGoogle Scholar
  31. Voß E, Weichgrebe D, Rosenwinkel KH (2009) FOS/TAC: Herleitung, Methodik, Anwendung und Aussagekraft. In: Proceedings of the international scientific conference biogas science 2009, vol 3, pp 675–682Google Scholar
  32. Walker M, Zhang Y, Heaven S, Banks CJ (2009) Potential errors in the quantitative evaluation of biogas production in anaerobic digestion processes. Bioresour Technol 100(24):6339–6346CrossRefGoogle Scholar
  33. Walker M, Iyer K, Heaven S, Banks CJ (2011) Ammonia removal in anaerobic digestion by biogas stripping: an evaluation of process alternatives using a first order rate model based on experimental findings. Chem Eng J 178:138–145.  https://doi.org/10.1016/j.cej.2011.10.027 CrossRefGoogle Scholar
  34. Yang F (2011) Mesophilic anaerobic digestion conducted in single unit reactor at increasing ammonia concentrations, Master thesis, Programme of Applied Environmental Science, Halmstad UniversityGoogle Scholar

Copyright information

© Islamic Azad University (IAU) 2019

Authors and Affiliations

  1. 1.Department of Public WorksFaculty of Engineering, Cairo UniversityGizaEgypt

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