Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate

Utilization of waste tobacco for cellulase production
  • A. V. BuntićEmail author
  • M. D. Milić
  • O. S. Stajković-Srbinović
  • N. V. Rasulić
  • D. I. Delić
  • K. R. Mihajlovski
Original Paper


This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 °C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl cellulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste.


Avicelase Carboxymethyl cellulase Lignocellulose Response surface method Rhizobium 



The financial support for this investigation given by the Ministry of Education, Science and Technological Development of the Republic of Serbia under the Project TR 31035 and TR 37006 is gratefully acknowledged.

Compliance with etical standard

Conflict of interes

The authors declare that they have no conflict of interest.


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Copyright information

© Islamic Azad University (IAU) 2019

Authors and Affiliations

  1. 1.Department of MicrobiologyInstitute of Soil ScienceBelgradeSerbia
  2. 2.Department of Biochemical Engineering and Biotechnology, Faculty of Technology and MetallurgyUniversity of BelgradeBelgradeSerbia

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