Bioconversion of agro-industrial effluents for polyhydroxyalkanoates production using Bacillus subtilis RS1

  • R. Rathika
  • V. Janaki
  • K. Shanthi
  • S. Kamala-KannanEmail author
Original Paper


The effectiveness of inexpensive agro-industrial wastes such as sugarcane molasses, paper mill effluent, and dairy effluent for the production of polyhydroxyalkanoates (PHAs) using Bacillus subtilis RS1 was assessed in this study. Laboratory-scale fermentations were carried out with both raw and pre-treated agro-industrial effluents, and maximum PHAs yield was observed in pre-treated effluents. Experimental variables such as pH, inoculum dose, and incubation time were further optimized to maximize PHAs yield, and the optimal conditions were identified as incubation time 48 h; pH 7; and inoculum dose 10% v/v. PHAs yield in pre-treated sugarcane molasses increased to 70.5% under optimal conditions. Fourier transform infrared spectroscopy and gas chromatography–mass spectrometric analysis revealed that the extracted polymer was composed of penta- and hexadecanoic acid methyl esters, a copolymer of PHAs. Results indicated that the pre-treated sugarcane molasses could be used as an inexpensive substrate for the production of PHAs.


Agro-industrial effluents Bacillus subtilis RS1 Fermentation Hexadecanoic acid Polyhydroxyalkanoates 



This paper was supported by research funds of Chonbuk National University in 2017. The author (SKK) is grateful to the Head and faculties of Department of Environmental Sciences, PSG College of Arts and Science, Coimbatore, Tamil Nadu, India for their valuable support.

Compliance with ethical standards

Conflict of interest

The authors declare that they do not have any conflict of interest.


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Copyright information

© Islamic Azad University (IAU) 2018

Authors and Affiliations

  1. 1.Department of Environmental SciencePSG College of Arts and ScienceCoimbatoreIndia
  2. 2.Department of ChemistrySri Sarada College for WomenSalemIndia
  3. 3.Division of Biotechnology, College of Environmental and Bioresource SciencesChonbuk National UniversityIksanSouth Korea

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