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Production of polyhydroxybutyrate from Acinetobacter nosocomialis RR20 strain using modified mineral salt medium: a statistical approach

  • A. R. Reddy
  • K. A. Peele
  • S. Krupanidhi
  • K. V. Prabhakar
  • T. C. VenkateswaruluEmail author
Short Communication
  • 89 Downloads

Abstract

Eco-friendly biopolymers, polyhydroxybutyrate, have been produced by many kinds of bacteria that possess most important applications in food packaging industries and also in medical field. In the present work, PHB production was economized through the statistical optimization of nutritional components. The preferable carbon and nitrogen sources chosen for the enhanced production of PHB were molasses and ammonium sulphate. The relative rate of production has been studied by understanding the complex interactions of variables using face-centred central composite design. The submerged fermentation with Acinetobacter nosocomialis RR20 produced PHB yield of 7.82 g/L at optimized conditions with a notable value change of eightfold increase in comparison with minimal salt medium and these findings have showed that the designed medium was significant in terms of higher of PHB production.

Keywords

Polyhydroxybutyrate Acinetobacter nosocomialis RR20 strain Response surface methodology Shake flask culture 

Notes

Acknowledgements

Authors would like to acknowledge the facilities supported by DST-FIST and VFSTR.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Islamic Azad University (IAU) 2018

Authors and Affiliations

  • A. R. Reddy
    • 1
  • K. A. Peele
    • 1
  • S. Krupanidhi
    • 1
  • K. V. Prabhakar
    • 2
  • T. C. Venkateswarulu
    • 1
    Email author
  1. 1.Department of Bio-TechnologyVignan’s Foundation for Science Technology & Research (Deemed to be University)Vadlamudi, GunturIndia
  2. 2.Department of Bio-TechnologyVikrama Shimahapuri UniversityNelloreIndia

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