Evaluation of butachlor biodegradation efficacy of Serratia ureilytica strain AS1: a statistical optimization approach
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Butachlor is a chloroacetanilide class of herbicide, commonly administered to control unwanted grasses and broad leaf weeds. Extensive usage of the herbicide has led to the contamination of water bodies and surrounding areas, resulting in an adverse impact on the environment. In the present work, a novel butachlor-catabolizing bacterium Serratia ureilytica strain AS-1 was isolated from an herbicide-contaminated soil. Statistical optimization techniques were used to optimize the butachlor biodegradation. Experimental parameters such as growth temperature, pH of the medium and biomass concentration were found to be significant for butachlor biodegradation. The results obtained indicates that the maximum degradation of 2.08 mg/L/h of butachlor was achieved under the optimal conditions of 32.5 °C of incubation temperature, pH 7.5 and 10% (v/v) inoculum size along with a polynomial mathematical model having R2 = 0.9833. The model was corroborated by carrying out experiments at the optimized conditions.
KeywordsBiodegradation Butachlor Environmental pollution Response surface methodology Serratia
The authors wish to thank all who assisted in conducting this work.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest regarding the subject of the manuscript.
- Ateeq B, Abul farah M, Niamat Ali M, Ahmad W (2002) Induction of micronuclei and erythrocyte alterations in the catfish Clarias batrachus by 2,4-dichlorophenoxyacetic acid and butachlor. Mutat Res Genet Toxicol Environ Mutagen 518:135–144. https://doi.org/10.1016/s1383-5718(02)00075-x CrossRefGoogle Scholar
- DAFP Odisha (2008) Manual on agricultural production technology. BhubaneswarGoogle Scholar
- Dwivedi S, Singh BR, Al-Khedhairy AA, Alarifi S, Musarrat J (2010) Isolation and characterization of butachlor-catabolizing bacterial strain Stenotrophomonas acidaminiphila JS-1 from soil and assessment of its biodegradation potential. Lett Appl Microbiol 51:54–60. https://doi.org/10.1111/j.1472-765x.2010.02854.x Google Scholar
- Hsu K-Y, Lin H-J, Lin J-K, Kuo W-S, Ou Y-H (2005) Mutagenicity study of butachlor and its metabolites using Salmonella typhimurium. J Microbiol Immunol Infect 38:409–416Google Scholar
- Mohanty SS (2012) Microbial degradation of phenol: a comparative study. National Institute of Technology, RourkelaGoogle Scholar
- Mohanty SS, Jena HM (2017) Biodegradation of phenol by free and immobilized cells of a novel Pseudomonas sp. NBM11. Braz J Chem Eng 34:75–84. https://doi.org/10.1590/0104-6632.20170341s20150388 CrossRefGoogle Scholar
- Pakala SB, Gorla P, Pinjari AB, Krovidi RK, Baru R, Yanamandra M, Merrick M, Siddavattam D (2007) Biodegradation of methyl parathion and p-nitrophenol: evidence for the presence of a p-nitrophenol 2-hydroxylase in a Gram-negative Serratia sp. strain DS001. Appl Microbiol Biotechnol 73:1452–1462. https://doi.org/10.1007/s00253-006-0595-z CrossRefGoogle Scholar
- Rahman MFA, Shukor MY, Suhaili Z, Mustafa S, Shamaan NA, Syed MA (2009) Reduction of Mo(VI) by the bacterium Serratia sp. strain DRY5. J Environ Biol 30:65–72Google Scholar
- Rajasankar R, Manju Gayathry G, Sathiavelu A, Ramalingam C, Saravanan VS (2013) Pesticide tolerant and phosphorus solubilizing Pseudomonas sp. strain SGRAJ09 isolated from pesticides treated Achillea clavennae rhizosphere soil. Ecotoxicology 22:707–717. https://doi.org/10.1007/s10646-013-1062-0 CrossRefGoogle Scholar
- Zhao X, Wang L, Ma F, Bai S, Yang J, Qi S (2017) Pseudomonas sp. ZXY-1, a newly isolated and highly efficient atrazine-degrading bacterium, and optimization of biodegradation using response surface methodology. J Environ Sci (China) 54:152–159. https://doi.org/10.1016/j.jes.2016.06.010 CrossRefGoogle Scholar