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Assessment and comparison of phytoremediation potential of selected plant species against endosulfan

  • V. SinghEmail author
  • A. Lehri
  • N. Singh
Original Paper
  • 59 Downloads

Abstract

The present study is focused on assessment of phytoremediation potential of selected plant species by removal of endosulfan from contaminated soil via plant uptake. Eight plant species were selected for pot experiment under controlled condition. From the field monitoring study, Vetiveria zizanioides was found to be accumulate more endosulfan as compared to others. In this experiment, the phytoremediation potential of V. zizanioides is further tested. Apart from V. zizanioides, eight locally available plant species, namely Phragmitis karka, Jatropha curcas, Brassica juncea, Vigna radiata, Solanum lycopersicum, Solanum melongena, Spinacia oleracea and Withania somnifera, were also tested against different concentrations of endosulfan (0–1500 μg g−1) the interval of at 500 μg g−1. Morphological parameters in terms of fresh weight, biomass, shoot length and root length were deliberate just after harvesting. The chlorophyll, carotenoids and lipid peroxidation were estimated in plant samples. Microbial biomass carbon (MBC), dehydrogenase activity (DHA), pH, electrical conductivity and endosulfan concentration were analyzed in soil before and after cropping. The result shows that there was a noteworthy difference at 95% confidence level in growth of experimental plants when compared with control. Enhanced MBC and DHA showed active degradation of endosulfan by microbes that proliferate due to secretion of root exudates of test plants. Among all the test plants, V. zizanioides accumulated the highest and B. juncea accumulated the lowest concentration of endosulfan in their tissues. No significant reduction in lipid peroxidation and chlorophyll content in V. zizanioides supports its suitability for phytoremediation.

Keywords

Accumulation Endosulfan Phytoremediation Vetiveria zizanioides Biomass 

Notes

Acknowledgements

The authors desire to show gratitude to Department of Science and Technology (DST) for economic support as WOS-A project (SR/WOS-A/LS-257/2009). The authors also desire to thank Director, Council of Scientific and Industrial Research—National Botanical Research Institute (CSIR-NBRI) for providing essential amenities.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Abaga NOZ, Dousset S, Munier-Lamy C, Billet D (2013) Effectiveness of Vetiver grass (Vetiveria zizanioides L. Nash) for phytoremediation of endosulfan in two cotton soils from Burkina Faso. Int J Phytorem 16(1):95–108CrossRefGoogle Scholar
  2. ADLG (Analytical Detection Limit Guidance) (1996) Analytical detection limit guidance & laboratory guide for determining method detection limits. Wisconsin Department of Natural Resources Laboratory Certification ProgramGoogle Scholar
  3. Aida M, Kozuyuki I, HiroakiI Naokuni H, Yasuo I, Kenji U (2006) Susceptibility of aquatic ferns to paddy herbicides bensulfuron methyl. Weed Biol Manag 4(3):127CrossRefGoogle Scholar
  4. Bacci E, Calamari D, Gaggi C, Vighi M (1990) Bioconcentration of organic chemical vapors in plant leaves: experimental measurements and correlation. Environ Sci Technol 24:885–889CrossRefGoogle Scholar
  5. Bacci E, Cerejeira MJ, Gaggi C, Chamello G, Calamari D, Vighi M (1992) Chlorinated dioxins: volatilization from soils and bioconcentration in plant leaves. Bull Environ Contam Toxicol 48:401–408CrossRefGoogle Scholar
  6. Battah MG, Shabana EF, Kobbia IA, Eladel HM (2001) Differential effects of thiobencarb toxicity on growth and photosynthesis of Anabaena variabilis with change in phosphate level. Ecotoxicol Environ Saf 49(3):235–239CrossRefGoogle Scholar
  7. Becerra-Castro C, Kidd PS, Rodríguez-Garrido B, Monterroso C, Santos-Ucha P, Prieto-Fernández A (2013) Phytoremediation of hexachlorocyclohexane (HCH)-contaminated soils using Cytisus striatus and bacterial inoculants in soils with distinct organic matter content. Environ Pollut 178:202–210CrossRefGoogle Scholar
  8. Bhadauria BS, Mathur VB, Kaul R (2012) Monitoring of organochlorine pesticides in and around Keoladeo National Park, Bharatpur, Rajasthan, India. Environ Monit Assess 184:5295–5300CrossRefGoogle Scholar
  9. Breusegem FV, James FD (2006) Reactive oxygen species in plant cell death. Plant Physiol 141(2):384–390CrossRefGoogle Scholar
  10. Calveno Pereira RC, Monterroso C, Macías F (2010) Phytotoxicity of hexachlorocyclohexane: effect on germination and early growth of different plant species. Chemosphere 79:326–333CrossRefGoogle Scholar
  11. Casida LE, Klein DA, Santoro T (1964) Soil dehydrogenase activity. Soil Sci 98:371–376CrossRefGoogle Scholar
  12. Chiou CT, Sheng G, Manes MA (2001) A partition-limited model for the plant uptake of organic contaminants from soil and water. Environ Sci Technol 35:1437–1444CrossRefGoogle Scholar
  13. Chou CS, Chang C, Kaw CI (1978) Impact of water pollution of crop growth in Taiwan. Bot Bull Bot Sinica 19:107–124Google Scholar
  14. Chouychai W, Hung L (2012) Phytotoxicity assay of crop plants to Lindane and alpha endosulfan contaminants in alkaline Thai soil. International journal of agriculture and biology 14:734–738Google Scholar
  15. Das P, Datta R, Makris KC, Sarkar D (2010) Vetiver grass is capable of removing TNT from soil in the presence of urea. Environ Pollut 158:1980–1983CrossRefGoogle Scholar
  16. Devi NL, Chakraborty P, Shihua Q, Zhang G (2013) Selected organochlorine pesticides (OCPs) in surface soils from three major states from the north eastern part of India. Environ Monit Assess 185(8):6667–6676CrossRefGoogle Scholar
  17. Duxbury AC, Yentsch CS (1956) Plankton pigment monographs. J Mar Res 15:92–101Google Scholar
  18. Eevers N, White JC, Vangronsveld J, Weyens N (2017) Bio-and Phytoremediation of pesticide-contaminated environments: a review. Adv Bot Res (in press). Corrected Proof, Available online 11 March 2017Google Scholar
  19. EL-Shahate RM, EL-Araby MMI, Eweda EW, El-Berashi MN (2011) Evaluation of the effect of three different pesticides on Azolla pinnata growth and NPK uptake. J Am Sci 7(1):1020–1031Google Scholar
  20. Enhelling FA, Muth MS, Schon MK (1985) Effect of allelochemicals on plant-water relationship. In: Thomson AC (ed) The chemistry of allelopathy. American Chemical Society, Washington, DCGoogle Scholar
  21. Fan S, Li P, Gong Z, Ren W, He N (2008) Promotion of pyrene degradation in rhizo-sphere of alfalfa (Medicago sativa L.). Chemosphere 71:1593–1598CrossRefGoogle Scholar
  22. Favas PJC, Pratas J, Varun M, D’Souza R, Paul MS (2014) Accumulation of uranium by aquatic plants in field conditions: prospects for phytoremediation. Sci Total Environ 470(1):993–1002CrossRefGoogle Scholar
  23. Gao Y, Zhu L (2004) Plant uptake accumulation and translocation of phenanthrene and pyrene in soils. Chemosphere 55:1169–1178CrossRefGoogle Scholar
  24. Heath RL, Packer L (1968) Photo peroxidation in isolated chloroplast I. Kinetic and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198CrossRefGoogle Scholar
  25. Hussain S, Arshad M, Saleem M, Khalid A (2007) Biodegradation of alpha and beta endosulfan by soil bacteria. Biodegradation 18:731–740CrossRefGoogle Scholar
  26. Ishtiaq S, Mahmood S (2011) Phytotoxicity of nickel and its accumulation in tissues of three Vigna species at their early growth stages. J Appl Bot Food Qual 84:223–228Google Scholar
  27. Kaushik CP, Sharma HR, Kaushik A (2012) Organochlorine pesticide residue in drinking water in the rural areas of the Haryana, India. Environ Monit Assess 184:103–112CrossRefGoogle Scholar
  28. Kwon GS, Kim JE, Kim TK, Sohn HY, Koh SC, Shin KS, Kim DG (2002) Klebsiella pneumonia KE-1 degrades endosulfan without formation of the toxic metabolite, endosulfan sulfate. FEMS Microbiol Lett 215:255–259CrossRefGoogle Scholar
  29. Kwon GS, Sohn HY, Shin KS, Kim E, Seo BI (2005) Biodegradation of the organochlorine insecticide, endosulfan, and the toxic metabolite, endosulfan sulfate, by Klebsiella oxytoca KE-8. Appl Microbiol Biotechnol 67:845–850CrossRefGoogle Scholar
  30. Loibner AP, Farthofer R, Braun R (1998) Aerobic degradation of Hexachlorocyclohexane isomers in soil monitored by using an online GC–MS system. In: Proceedings of the 4th international symposium and exhibition on environmental contamination on central and eastern Europe, Warsaw, pp 548–552Google Scholar
  31. Lu JLDP (2010) Multi pesticide residue assessment of agricultural soil and water in major farming areas in Benguet, Philippines. Arch Environ Contam Toxicol 59:175–181CrossRefGoogle Scholar
  32. Machalachlan S, Zalik S (1963) Plastid structure, chlorophyll concentration and free amino acid composition of a chlorophyll mutant of barley. Can J Bot 41:1053–1062CrossRefGoogle Scholar
  33. Makris KC, Shakya M, Datta R, Sarkar D, Pachanoor D (2007) High uptake of 2,4,6-trinitrotoluene by vetiver grass potential for phytoremediation. Environ Pollut 146:1–4CrossRefGoogle Scholar
  34. Marcacci S, Raveton M, Ravanel P, Schwitzguebel JP (2006) Conjugation of atrazine in vetiver (Chrysopogon zizanioides Nash) grown in hydroponics. Environ Exp Bot 56:205–215CrossRefGoogle Scholar
  35. Masood A, Zeeshan M, Abraham G (2008) Acta Biol Hung 247–257. Aida M, Ikeda H Itoh K, Usui K Ecotox Environ Saf 63:463–468Google Scholar
  36. Menezes RG, Qadir TF, Moin A, Fatima H, Senthilkumaran S (2017) Endosulfan poisoning: an overview. J Forensic Leg Med 51:27–33CrossRefGoogle Scholar
  37. Mersie W, Singh M (1993) Phenolic acid affect photosynthesis and protein synthesis by isolated leaf cells of velvet-leaf. J Chem Ecol 19:1293–1297CrossRefGoogle Scholar
  38. Mishra S, Srivastava S, Tripathi RD, Govindrajan R, Kuriakose SV, Prasda MNV (2006) Phytocheletin synthesis and response of antioxidants during cadmium stress in Bacopa monnieri L. Plant Physiol Biochem 44:25–37CrossRefGoogle Scholar
  39. Nakajima DJY, Suzuki J, Suzuki S (1995) Seasonal changes in the concentration of polycyclic aromatic hydrocarbons in azalea leaves and relationship to the atmospheric concentration. Chemosphere 30:409–418CrossRefGoogle Scholar
  40. Paterson S, Mackay D (1994) A model of organic chemical uptake by plants from soil and the atmosphere. Environ Sci Technol 28:2259–2265CrossRefGoogle Scholar
  41. Polder MD, Hulzebos EM, Jager DT (1995) Validation of models on uptake of organic chemicals by plant roots. Environ Toxicol Chem 14(1615):1623Google Scholar
  42. POPRC (2009) Decision POPRC-4/5 on endosulfan fulfilling the screening criteria of the Stockholm Convention. http://www.pops.int POPRC-4 meeting report. Antonious GF, Byers ME (1997) Fate and movement of endosulfan under field conditions. Environ Toxicol Chem 16:644–649
  43. Quilchano C, Maranon T (2002) Dehydrogenase activity in mediterranean forest soils. Biol Fertil Soils 35:102–107CrossRefGoogle Scholar
  44. Rice CP, Chernyak SM, Hapeman CJ, Biboulian S (1997) Air–water distribution of the endosulfan isomers. J Environ Qual 26:1101–1106CrossRefGoogle Scholar
  45. Schmidt WF, Bilboulian S, Rice CP, Fettinger JC, McConnell LL, Hapeman CJ (2001) Thermodynamic, spectroscopic, and computational evidence for the irreversible conversion of alpha to beta-endosulfan. J Agric Food Chem 49:5372–5376CrossRefGoogle Scholar
  46. Scroll R, Bierling B, Cao G, Dorfler U, Lahanaiti M (1994) Uptake pathway of organic chemicals from soil by agricultural plants. Chemosphere 28:297–303CrossRefGoogle Scholar
  47. Sengupta PK, Chakraborti A, Banerjee SK (1986) Biochemical changes induced by toxic concentration of malathion in germinating wheat seeds. Curr Sci 55:492–494Google Scholar
  48. Sethunathan N, Megharaj M, Chen ZL, Williams BD, Lewis G, Naidu R (2004) Algal degradation of a known endocrine disrupting insecticide, endosulfan and its metabolite, endosulfan sulfate in liquid medium and soil. J Agric Food Chem 52:3030–3035CrossRefGoogle Scholar
  49. Shaikh IR, Shaikh PR, Shaikh RA, Shaikh AS (2013) Phytotoxic effects of heavy metals (Cr, Cd, Mn and Zn) on wheat (Triticum aestivum L.) seed germination and seedlings growth in black cotton soil of Nanded, India. Res J Chem Sci 3(6):14–23Google Scholar
  50. Siddique ZS, Ahmad S (2000) Effect of synthetic fungicide on nutritive composition of diseased and healthy plants of Triticum aestivum L. Pak J Biol Sci 3:2148–2150CrossRefGoogle Scholar
  51. Siddique ZS, Ahmad S, Gulzar S (1997) Effect of topsin-M(Methyl-thiophenate) and bayleton (Triademifon) on seedling growth, biomass, nodulation and phenolic content of Sesbania sesban. Bangladesh J Bot 26:127–130Google Scholar
  52. Simonich SL, Hites RA (1994) Vegetation atmosphere partitioning of polycyclic aromatic hydrocarbons. Environ Sci Technol 28:939–943CrossRefGoogle Scholar
  53. Singh V, Singh N (2014) Uptake and accumulation of endosulfan isomers and its metabolite endosulfan sulfate in naturally growing plants of contaminated area. Ecotoxicol Environ Saf 104:189–193CrossRefGoogle Scholar
  54. Singh BK, Munro S, Reid E, Ord B, Potts JM, Patterson E, Millard P (2006) Investing microbial community structure in soils by physiological, biochemical and molecular fingerprinting methods. Eur J Soil Sci 57:72–82CrossRefGoogle Scholar
  55. Singh V, Singh P, Singh N (2016) Synergistic influence of Vetiveria zizanioides and selected rhizospheric microbial strains on remediation of endosulfan contaminated soil. Ecotoxicology 25(7):1327–1337CrossRefGoogle Scholar
  56. Sinha S, Mallick S, Mishra RK, Singh S, Basant A, Gupta AK (2007) Uptake and translocation of metals in Spinacia oleracea L. grown on tannery sludge—amended and contaminated soils: effect on lipid peroxidation, morpho anatomical changes and anti oxidants. Chemosphere 67:176–187CrossRefGoogle Scholar
  57. Stepniewska Z, Wolinska A (2005) Soil dehydrogenase activity in the presence of chromium (III) and (VI). Int Agrophys 19:79–83Google Scholar
  58. Trapp S, Matthies M, Scheunert I, Topp EM (1990) Modeling the bioconcentration of organic chemicals in plants. Environ Sci Technol 24(8):1246–1252CrossRefGoogle Scholar
  59. Turner RC, Marshal C (1972) Accumulation of zinc by subcellular fraction of some root argotic teneys in relation to zinc tolerance. New Phyton 71:671–676CrossRefGoogle Scholar
  60. Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass carbon. Soil Biol Biochem 19:703–707CrossRefGoogle Scholar
  61. Walse SS, Scott GI, Ferry JL (2003) Stereoselective degradation of aqueous endosulfan in modular estuarine mesocosms: formation of endosulfan γ hydroxycarboxylate. J Environ Monit 5:373–579CrossRefGoogle Scholar
  62. Wang M, Jones KC (1994) Uptake of chlorobenzenes by carrots from spiked and sewage sludge-amended soil. Environ Sci Technol 28:1260–1267CrossRefGoogle Scholar
  63. Wang MC, Chen YT, Chen SH, Chien SWC, Sunkara SV (2012) Phytoremediation of pyrene contaminated soils amended with compost and planted with ryegrass and alfalfa. Chemosphere 87(3):217–225CrossRefGoogle Scholar
  64. Welsch-Pausch K, McLachlan MS, Umlauf G (1995) Determination of the principal pathways of polychlorinated dibenzo-p-dioxins and dibenzofurans to Lolium multiflorum (Welsh Ray Grass). Environ Sci Technol 29:1090–1098CrossRefGoogle Scholar
  65. Wieczorek JK, Wieczorek ZJ (2007) Phytotoxicity and accumulation of anthracene applied to the foliage and sandy substrate in lettuce and radish plants. Ecotoxicol Environ Saf 66:369–377CrossRefGoogle Scholar
  66. Willet KL, Utrich EM, Hites RA (1998) Differential toxicity and environmental facts of hexachlorocyclohexane isomers. Environ Sci Technol 32:2197–2207CrossRefGoogle Scholar
  67. Xie HJ, Gao FW, Tan W, Wang SG (2011) A short-term study on the interaction of bacteria, fungi and endosulfan in soil microcosm. Sci Total Environ 412–413:375–379CrossRefGoogle Scholar

Copyright information

© Islamic Azad University (IAU) 2018

Authors and Affiliations

  1. 1.Central Instrumentation FacilityNational Botanical Research Institute (Council of Scientific and Industrial Research)LucknowIndia
  2. 2.Plant Ecology and Environmental Science DivisionNational Botanical Research Institute (Council of Scientific and Industrial Research)LucknowIndia

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