A central composite face-centered design for optimizing the detection of Salmonella typhi with a fluorescence nanobiosensor using the microcontact method
The rapid detection of Salmonella typhi can be accomplished by developing nanobiosensor kits. Accordingly, in the present study, a molecularly imprinted polymer (MIP) was developed, formed via covalent bonds among methacrylic acid (MAA) monomers and hydrogen bonds between MAA and antibodies. Using this MIP, S. typhi antigen was detected with a fluorescence converter. The S. typhi concentration could be determined at 10 colony/mL (minimum) in polluted water. A spectrofluorometer was used to quantify the findings. In addition, Escherichia coli was inseminated in an aqueous environment containing S. typhi, and no interference with the sensor function was found. The sensor sensitivity was measured for 60 days, and the sensor performance was verified for 56 days. Thereafter, the performance diminished. The present findings indicated the sensitive and precise role of the sensor in detecting S. typhi antigens at concentrations of 10−1–109.
KeywordsNanosensor Microcontact Salmonella typhi Spectrofluorometry
We would like to thank the Nanolab of Food and Drug Organization, Ministry of Health. In addition, we extend our gratitude to Metallurgical Engineering Lab of Amirkabir University and Mabna Food Product Laboratory for their assistance.
Compliance with ethical standards
Conflict of interests
All authors declare that they have no conflict of interests.
- Ahari H, Razavilar V, Akbari B, Motallebi A, Anvar A, Mohammadi N (2014) Comparison of potentiometry and spectroscopy-based modified nanoparticles linked to antibodies for detection of Staphylococcus aureus exotoxin. Iran J Nutr Sci Food Technol 8(4):247–258Google Scholar
- Anvar SA, Razavilar V, Akbari B, Motallebi A (2014) Detection of Salmonella typhi in aquatic environment using nano biosensor based on fluorescence. Int J Biol Pharm Allied Sci 3(11):2595–2615Google Scholar
- Foroshani NS, Karami A, Pourali F (2013) Simultaneous and rapid detection of Salmonella typhi, Bacillus anthracis, and Yersinia pestis by using multiplex polymerase chain reaction (PCR). Iran Red Crescent Med J 15(11):e9208Google Scholar
- Sankar S, Vadivel K, Nandagopal B, Jesudason MV, Sridharan G (2014) A multiplex nested PCR for the simultaneous detection of Salmonella typhi, Mycobacterium tuberculosis, and Burkholderia pseudomallei in patients with pyrexia of unknown origin (PUO) in Vellore, South India. Mol Diagn Ther 18(3):315–321CrossRefGoogle Scholar