The microbiological response of forest soils after application of nicosulfuron, imazamox and cycloxydim

  • V. VasicEmail author
  • S. Djuric
  • T. Jafari-Hajnal
  • S. Orlovic
  • S. Vasic
  • L. Poljakovic Pajnik
  • V. Galović
Original Paper


During application, a significant part of herbicide reaches the soil surface and profile which inevitably affect the activity of microorganisms. Some microorganisms are able to degrade herbicides and use them as a source of biogenic elements and energy to maintain their physiological processes. On the other hand, herbicides may alter the number of microorganisms as well as change their activity and reproduction. For that reason, it is of most importance to determine the correlation between microorganisms and herbicides in the soil. The aim of this study was to examine the effect of herbicides nicosulfuron, imazamoxare and cycloxydim, applied in the regeneration of oak forests on different groups of microorganisms in the soil. Microbiological research included the determination of the total number of bacteria, fungi, actinomycetes, azotobacter and aminoheterotrophs. The number of microorganisms was determined by introducing a diluted soil suspension into the proper media and counted per 1 g of absolutely dry soil. The investigated herbicides cycloxydim, nicosulfuron and imazamoxare reduced the total number of bacteria and aminoheterothrops but increased the number of actinomycetes and fungi in forest soils. Imazamox and cycloxydim had a negative effect on the Azotobacter sp., while the effect of nicosulfuron was stimulative.


Herbicides Microorganisms Soil Oak forest 



This paper was realised as a part of the project “Studying climate change and its influence on the environment: impacts, adaptation and mitigation” (43007) financed by the Ministry of Education and Science of the Republic of Serbia.


  1. Allison SD, LeBauer DS, Ofrecio MR, Reyes R, Ta A-M, Tran TM (2009) Low levels of nitrogen addition stimulate decomposition by boreal forest fungi. Soil Biol Biochem 41:293–302CrossRefGoogle Scholar
  2. Anderson GR (1965) Ecology of Azotobacter in soil of the palouse region I: occurrence. Soil Sci 86:57–65CrossRefGoogle Scholar
  3. Busse MD, Ratcliff WA, Shestak CJ (2001) Glyphosate toxicity and the effects of long-term vegetation control on soil microbial communities. Soil Biol Biochem 33(12–13):1777–1789CrossRefGoogle Scholar
  4. Crouzet O, Batisson I, Besse-Hoggan P, Bonnemoy F, Bardot C, Poly F, Bohatier J, Mallet C (2010) Response of soil microbial communities to the herbicide mesotrione: a dose-effect microcosm approach. Soil Biol Biochem 42:193–202CrossRefGoogle Scholar
  5. Dixon LF, Clay VD (2004) Effect of herbicides applied pre-and post-emergence on forestry weeds grown from seed. Crop Prot 23:713–721CrossRefGoogle Scholar
  6. Edwards CA (1989) Impact of herbicides on soil ecosystems. Crit Rev Plant Sci 8:221–253CrossRefGoogle Scholar
  7. Egner H, Riehm H, Domingo WR (1960) Untersuchungen über die chemische Bodenanalyse als Grundlage für die Beurteilung des Nahrstoffzustandes der Boden, II: chemische Extractionsmetoden zu Phosphorund Kaliumbestimmung. Kungliga Lantbrukshügskolans Annaler 26:199–215Google Scholar
  8. Entry JA, Donelly PK, Emmingham WH (1994) Microbial mineralization of atrazine and 2,4-dichlorophenoxyacetic acid in riparian and forest soils. Biol Fertil Soils 18:89–94CrossRefGoogle Scholar
  9. Group of authors (1997) Metode istraživanja i određivanja fizičkih svojstava zemljišta, Priručnik za ispitivanje zemljišta (in serbian). JDPZ, Novi Sad, p 278Google Scholar
  10. Imfeld G, Vuilleumier S (2012) Measuring the effects of pesticides on bacterial communities in soil: a critical review. Eur J Soil Biol 49:22–30CrossRefGoogle Scholar
  11. Jarak M, Djuric S (2006) Praktikum iz mikrobiologije. Univerzitet u Novom Sadu, Poljoprivredni fakultet, Novi SadGoogle Scholar
  12. Kizildag N, Sagliker H, Cenkseven S, Cengiz Darici H, Kocak B (2014) Effects of imazamox on soil carbon and nitrogen mineralization under Mediterranean climate. Turk J Agric For 38:334–339CrossRefGoogle Scholar
  13. Lebron I, Robinson AD, Oatham M, Wuddivira NM (2012) Soil water repellency and pH soil change under tropical pine plantations compared with native tropical forest. J Hydrol 414–415:194–200CrossRefGoogle Scholar
  14. Lewis KA, Newbold MJ, Hall AM, Broom CE (1997) Eco-rating system for optimizing pesticide use at farm level—Part l: theory and development. J Agric Eng Res 68:271–279CrossRefGoogle Scholar
  15. Lindahl BD, Ihrmark K, Boberg J, Trumbore SE, Hogberg P, Stenlid J, Finlay RD (2007) Spatial separation of litter decomposition and mycorrhizal nitrogen uptake in a boreal forest. New Phytol 173:611–620CrossRefGoogle Scholar
  16. Liu W, Xu W, Hong J, Wan S (2010) Inter annual variability of soil microbial biomass and respiration in responses to topography, annual burning and N addition in a semiarid temperate steppe. Geoderma 158:259–267CrossRefGoogle Scholar
  17. Lupwayi NZ, Harker KN, Clayton GW, Turkington TK, Rice WA, O’Donovan JT (2004) Soil microbial biomass and diversity after herbicide application. Can J Plant Sci 84:677–685CrossRefGoogle Scholar
  18. Martinez-Toledo MV, Salmeron V, Gonzalez-Lopez J (1990) Metolachlor and the biological activity of Azotobacter chroococcum. Soil Biol Biochem 22:123–125CrossRefGoogle Scholar
  19. Niewiadomska A, Sawicka A (2002) Effect of carbendazim, imazetapir and thiram on nitrogenase activity, number of microorganisms in soil and yield of hybrid Lucerne. Pol J Environ Stud 11:737–744Google Scholar
  20. Oleszczuk P, Malara A, Jośko I, Lesiuk A (2012) The phytotoxicity changes of sewage sludge-amended soils. Water Air Soil Poll 223:4937–4948CrossRefGoogle Scholar
  21. Parkinson D (1982) Filamentous fungi. In: Page AE (ed) Methods of soil analysis, Part 2. Chemical and microbiological properties. American Society of Agronomy, Madison, pp 949–966Google Scholar
  22. Pozo C, Martinez-Toledo MV, Salmeron V, Rodales B, Gonzalez-Lopez J (2000) Effects of benzedine and benzedine analogues on the growth and nitrogenase activity of Aazotobacter. Appl Soil Ecol 14:183–190CrossRefGoogle Scholar
  23. Ramanad K, Sharmila M, Sethunathan N (1988) Mineralization of carbofuran by soil bacterium. Appl Environ Microb 54:2129–2133Google Scholar
  24. Ratcliff AW, Busse MD, Shestak CJ (2006) Changes in microbial community structure following herbicide (glyphosate) additions to forest soils. Appl Soil Ecol 34:114–124CrossRefGoogle Scholar
  25. Rhine ED, Fuhrmann JJ, Radosevich M (2003) Microbial community responses to atrazine exposure and nutrient availability: linking degradation capacity to community structure. Microb Ecol 46:145–160CrossRefGoogle Scholar
  26. Scharlau Microbiology (1999) Handbook of Microbiogical Culture Media. Ref.1-219: 346, Fifth International Edition, BarcelonaGoogle Scholar
  27. Seghers D, Verthe K, Reheul D, Bulcke R, Siciliano DS, Verstraete W, Top ME (2003) Effect of long-term herbicide applications on the bacterial community structure and function in an agricultural soil. FEMS Microbiol Ecol 46:139–146CrossRefGoogle Scholar
  28. Shen DS, Fang CR, He R, Zhu YM (2006) Effects of metsulfuron-methyl on the microbial population and enzyme activities in wheat rhizosphere soil. J Environ Sci Health B 41:269–284CrossRefGoogle Scholar
  29. Simakov VN (1957) The use of phenylanthranilic acid in the determination of humus by Tyurin’s method. Pochvovedenie 8:72–73Google Scholar
  30. Srivastava J, Naraian R, Kalra SJS, Chandra H (2014) Advances in microbial bioremediation and the factors influencing the process. Int J Environ Sci Technol 11(6):1787–1800CrossRefGoogle Scholar
  31. Trolldenier G (1996) Plate Count Technique. In: Schinner F, Kandeler E, Ohlinger R, Margesin R (eds) Methods in soil biology. Springer, Berlin, pp 20–26Google Scholar
  32. Vasic V, Konstantinovic B, Orlovic S (2014) Application of post-emergence herbicides in the regeneration of pedunculate oak (Quercus robur L.) forests. Forestry 87:407–415CrossRefGoogle Scholar
  33. Veiga F, Zapata MJ, Fernandez Marcos LM, Alvarez E (2001) Dynamics of glyphosate and aminomethylphosphonic acid in a forest soil in Galicia, north-west Spain. Sci Total Environ 271:135–144CrossRefGoogle Scholar

Copyright information

© Islamic Azad University (IAU) 2018

Authors and Affiliations

  • V. Vasic
    • 1
    Email author
  • S. Djuric
    • 2
  • T. Jafari-Hajnal
    • 2
  • S. Orlovic
    • 1
  • S. Vasic
    • 1
  • L. Poljakovic Pajnik
    • 1
  • V. Galović
    • 1
  1. 1.Institute of Lowland Forestry and EnvironmentUniversity of Novi SadNovi SadSerbia
  2. 2.Department for Environmental and Plant Protection, Faculty of AgricultureUniversity of Novi SadNovi SadSerbia

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