Advertisement

Electrochemical performance of biocathode microbial fuel cells using petroleum-contaminated soil and hot water spring

  • Z. Zafar
  • K. Ayaz
  • M. H. Nasir
  • S. Yousaf
  • I. Sharafat
  • N. AliEmail author
Original Paper
  • 173 Downloads

Abstract

Microbial fuel cells is growing technology for energy production (electrical and gaseous) with potential electrochemically active bacteria from degradation of unwanted contaminants. Electrogenic [petroleum-contaminated soil (PCS) and hot spring water HSW)] and electrotrophic [activated sludge] bacterial communities were enriched and evaluated for electric current production in biocathode microbial fuel cells (MFC). Molecular phylogenetic (454 pyrosequencing) analysis of environmental samples revealed an overall change in bacterial density and diversity after second-stage enrichment. The predominant electrogenic bacteria grown at anodic biofilms belonged to phylum Proteobacteria (80–98%) in both MFC-1 (PCS) and MFC-2 (HSW) reactors. After enrichment, the major shift in the bacterial species on anodic surface was observed in case of Stenotrophomonas maltophilia (89%) and shewanella sp. (15%) in the respective reactors. Overall, among electrotrophic bacteria, the relative abundance (27–30%) of Pseudomonas aeruginosa was maximum on the cathodic biofilm in both fuel cells. Scanning electron and confocal laser scanning microscopies of biofilms revealed that anode and cathode surfaces were covered with different microcolonies and dispersed bacterial cells. Cyclic voltammetry (− 1 to 1 V vs. Ag/AgCl) further confirmed the presence of highly proficient electrogenic bacteria capable of generating high electricity ranging from ≥ 8 mA in MFC-1 and ≤ 0.37-Y in MFC-2. Maximum power density of 5500 mW m−2 at a current density of 100 mA m−2 (550 Ω)] was recorded in MFC-1 during enrichment stage 2; however, it (Pmax  = 1201 mW m2) remained 78% lower in MFC-2. Fourier transform infrared spectroscopy and COD removal [86% (SD = 8.3 ± 2.0)] of anolyte (PCS) confirmed active degradation of petroleum contaminants during the operation of MFC-1.

Keywords

Electrogenic bacteria Biofilm Electric current Metagenomics Biodegradation 

Notes

Acknowledgements

This research was supported and funded by Department of Microbiology, Quaid-i-Azam University Islamabad and Higher Education Commission Islamabad, Pakistan.

Compliance with ethical standards

Conflicts of interest

It is declared that all authors have no conflict of interest.

Supplementary material

13762_2018_1757_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 16 kb)

References

  1. Al-Mamun A, Lefebvre O, Baawain MS, Ng HY (2016) A sandwiched denitrifying biocathode in a microbial fuel cell for electricity generation and waste minimization. Int J Environ Sci Technol 13(4):1055–1064CrossRefGoogle Scholar
  2. Al-Shehri A (2015) Employment of microbial fuel cell technology to biodegrade naphthalene and benzidine for bioelectricity generation International Journal of Current Microbiology and Applied Sciences 4:134–149Google Scholar
  3. BERGEY DHB et al. (1974) Bergey’s manual of determinative bacteriology RIA Revista de Investigaciones Agropecuarias Vol 27, no 2 (1996), p 157–167Google Scholar
  4. Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Lozupone CA, Turnbaugh PJ et al (2011) Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. PNAS 15:4516–4522CrossRefGoogle Scholar
  5. Chen J, Deng F, Hu Y, Sun J, Yang Y (2015) Antibacterial activity of graphene-modified anode on Shewanella oneidensis MR-1 biofilm in microbial fuel cell. J Power Sources 290:80–86CrossRefGoogle Scholar
  6. Choudhury P, Prasad Uday US, Bandyopadhyay TK, Ray RN, Bhunia B (2017a) Performance improvement of microbial fuel cell (MFC) using suitable electrode and Bioengineered organisms: a review. Bioengineered 8(5):471–487CrossRefGoogle Scholar
  7. Choudhury P, Uday USP, Mahata N, Tiwari ON, Ray RN, Bandyopadhyay TK, Bhunia B (2017b) Performance improvement of microbial fuel cells for waste water treatment along with value addition: a review on past achievements and recent perspectives. Renew Sustain Energy Rev 79:372–389CrossRefGoogle Scholar
  8. DeSantis TZ, Hugenholtz P, Larsen N, Rojas M, Brodie EL, Keller K, Huber T, Dalevi D, Hu P, Andersen GL (2006) Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl Environ Microbiol 72(7):5069–5072CrossRefGoogle Scholar
  9. Du Y, Feng Y, Dong Y, Qu Y, Liu J, Zhou X, Ren N (2014) Coupling interaction of cathodic reduction and microbial metabolism in aerobic biocathode of microbial fuel cell. RSC Advances 4:34350.  https://doi.org/10.1039/c4ra03441d CrossRefGoogle Scholar
  10. Ghangrekar M, Shinde V (2007) Performance of membrane-less microbial fuel cell treating wastewater and effect of electrode distance and area on electricity production. Bioresour Technol 98:2879–2885CrossRefGoogle Scholar
  11. Huang L, Regan JM, Quan X (2011) Electron transfer mechanisms, new applications, and performance of biocathode microbial fuel cells. Bioresour Technol 102:316–323CrossRefGoogle Scholar
  12. Jang JK, Pham TH, Chang IS, Kang KH, Moon H, Cho KS, Kim BH (2004) Construction and operation of a novel mediator- and membrane-less microbial fuel cell. Process Biochem 39:1007–1012.  https://doi.org/10.1016/S0032-9592(03)00203-6 CrossRefGoogle Scholar
  13. Juhasz AL, Britz M, Stanley G (1997a) Degradation of fluoranthene, pyrene, benz [a] anthracene and dibenz [a, h] anthracene by Burkholderia cepacia. J Appl Microbiol 83:189–198CrossRefGoogle Scholar
  14. Juhasz AL, Britz ML, Stanley GA (1997b) Degradation of benzo [a] pyrene, dibenz [a, h] anthracene and coronene by Burkholderia cepacia. Water Sci Technol 36:45–51CrossRefGoogle Scholar
  15. Juhasz AL, Stanley GA, Britz ML (2000) Microbial degradation and detoxification of high molecular weight polycyclic aromatic hydrocarbons by Stenotrophomonas maltophilia strain VUN 10,003. Lett Appl Microbiol 30:396–401CrossRefGoogle Scholar
  16. Kamau JM, Mwaniki JM, Mwaura FB, Kamau GN (2017) Microbial fuel cells: influence of external resistors on power. Curr Power Density J Thermodyn Catal.  https://doi.org/10.4179/2160-7544.1000182 CrossRefGoogle Scholar
  17. Kargi F, Eker S (2009) High power generation with simultaneous COD removal using a circulating column microbial fuel cell. J Chem Technol Biotechnol 84:961–965CrossRefGoogle Scholar
  18. Katuri KP, Scott K, Head IM, Picioreanu C, Curtis TP (2011) Microbial fuel cells meet with external resistance. Bioresour Technol 102:2758–2766.  https://doi.org/10.1016/j.biortech.2010.10.147 CrossRefGoogle Scholar
  19. Katuri KP, Enright A-M, O’Flaherty V, Leech D (2012) Microbial analysis of anodic biofilm in a microbial fuel cell using slaughterhouse wastewater. Bioelectrochemistry 87:164–171CrossRefGoogle Scholar
  20. Kim JR, Min B, Logan BE (2005) Evaluation of procedures to acclimate a microbial fuel cell for electricity production. Appl Microbiol Biotechnol 68:23–30CrossRefGoogle Scholar
  21. Lee TK, Van Doan T, Yoo K, Choi S, Kim C, Park J (2010) Discovery of commonly existing anode biofilm microbes in two different wastewater treatment MFCs using FLX Titanium pyrosequencing. Appl Microbiol Biotechnol 87:2335–2343CrossRefGoogle Scholar
  22. Lefebvre O, Al-Mamun A, Ng H (2008) A microbial fuel cell equipped with a biocathode for organic removal and denitrification. Water Sci Technol 58:881–885CrossRefGoogle Scholar
  23. Lin H, Wu X, Miller C, Zhu J (2013) Improved performance of microbial fuel cells enriched with natural microbial inocula and treated by electrical current. Biomass Bioenergy 54:170–180.  https://doi.org/10.1016/j.biombioe.2013.03.030 CrossRefGoogle Scholar
  24. Lovley DR, Phillips EJ (1988) Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganese. Appl Environ Microbiol 54:1472–1480Google Scholar
  25. Lyon DY, Buret F, Vogel TM, Monier J-M (2010) Is resistance futile? Changing external resistance does not improve microbial fuel cell performance. Bioelectrochemistry 78:2–7.  https://doi.org/10.1016/j.bioelechem.2009.09.001 CrossRefGoogle Scholar
  26. McLean JS et al (2010) Quantification of electron transfer rates to a solid phase electron acceptor through the stages of biofilm formation from single cells to multicellular communities. Environ Sci Technol 44:2721–2727CrossRefGoogle Scholar
  27. Miceli JF III, Parameswaran P, Kang D-W, Krajmalnik-Brown R, Torres CI (2012) Enrichment and analysis of anode-respiring bacteria from diverse anaerobic inocula. Environ Sci Technol 46:10349–10355Google Scholar
  28. Milner EM, Popescu D, Curtis T, Head IM, Scott K, Eileen HY (2016) Microbial fuel cells with highly active aerobic biocathodes. J Power Sources 324:8–16CrossRefGoogle Scholar
  29. Parkash A (2015) Generation of bioenergy from sewage using dual chamber microbial fuel cell. J Bioprocess Biotechn 5(7):1Google Scholar
  30. Rahimnejad M, Adhami A, Darvari S, Zirepour A, Oh S-E (2015) Microbial fuel cell as new technology for bioelectricity generation: a review. Alex Eng J 54:745–756CrossRefGoogle Scholar
  31. Rimboud M, Desmond-Le Quemener E, Erable B, Bouchez T, Bergel A (2015) The current provided by oxygen-reducing microbial cathodes is related to the composition of their bacterial community. Bioelectrochemistry 102:42–49.  https://doi.org/10.1016/j.bioelechem.2014.11.006 CrossRefGoogle Scholar
  32. Rismani-Yazdi H, Christy AD, Carver SM, Yu Z, Dehority BA, Tuovinen OH (2011) Effect of external resistance on bacterial diversity and metabolism in cellulose-fed microbial fuel cells. Biores Technol 102:278–283CrossRefGoogle Scholar
  33. Saba B, Christy AD, Yu Z, Co AC, Islam R, Tuovinen OH (2017) Characterization and performance of anodic mixed culture biofilms in submersed microbial fuel cells. Bioelectrochemistry 113:79–84CrossRefGoogle Scholar
  34. Sabina K et al (2014) Microbial desalination cell for enhanced biodegradation of waste engine oil using a novel bacterial strain Bacillus subtilis moh3. Environ Technol 35:2194–2203.  https://doi.org/10.1080/09593330.2014.896951 CrossRefGoogle Scholar
  35. Salar-García MJ, Gajda I, Ortiz-Martínez VM, Greenman J, Hanczyc M, de Los Ríos A, Ieropoulos I (2016) Microalgae as substrate in low cost terracotta-based microbial fuel cells: novel application of the catholyte produced. Bioresour Technol 209:380–385CrossRefGoogle Scholar
  36. Sawasdee V, Pisutpaisal N (2016) Simultaneous pollution treatment and electricity generation of tannery wastewater in air-cathode single chamber MFC. Int J Hydrog Energy 41:15632–15637CrossRefGoogle Scholar
  37. Sen SK, Raut S, Satpathy S, Rout PR, Bandyopadhyay B, Das Mohapatra PK (2014) Characterizing novel thermophilic amylase producing bacteria from Taptapani Hot Spring, Odisha, India. Jundishapur J Microbiol 7(12):e11800.  https://doi.org/10.5812/jjm.11800 CrossRefGoogle Scholar
  38. Seo J-S, Keum Y-S, Li QX (2009) Bacterial degradation of aromatic compounds. Int J Environ Res Public Health 6:278–309CrossRefGoogle Scholar
  39. Strycharz-Glaven SM, Glaven RH, Wang Z, Zhou J, Vora GJ, Tender LM (2013) Electrochemical investigation of a microbial solar cell reveals a nonphotosynthetic biocathode catalyst. Appl Environ Microbiol 79:3933–3942CrossRefGoogle Scholar
  40. Sun Y, Wei J, Liang P, Huang X (2012) Microbial community analysis in biocathode microbial fuel cells packed with different materials. AMB express 2:1CrossRefGoogle Scholar
  41. The Issues Affecting Global Poverty (2013)Google Scholar
  42. Walters W, Hyde ER, Berg-Lyons D, Ackermann G, Humphrey G, Parada A, Gilbert JA, Jansson JK, Caporaso JG, Fuhrman JA, Apprill A (2016) Improved bacterial 16S rRNA gene (V4 and V4-5) and fungal internal transcribed spacer marker gene primers for microbial community surveys. Systems 1(1):e00009–15Google Scholar
  43. Wang M, Yan Z, Huang B, Zhao J, Liu R (2013) Electricity generation by microbial fuel cells fuelled with Enteromorpha prolifera hydrolysis. Int J Electrochem Sci 8:2104–2111Google Scholar
  44. Xia X, Tokash JC, Zhang F, Liang P, Huang X, Logan BE (2013a) Oxygen-reducing biocathodes operating with passive oxygen transfer in microbial fuel cells. Environ Sci Technol 47:2085–2091CrossRefGoogle Scholar
  45. Xia X, Tokash JC, Zhang F, Liang P, Huang X, Logan BE (2013b) Oxygen-reducing biocathodes operating with passive oxygen transfer in microbial fuel cells. Environ Sci Technol 47:2085–2091.  https://doi.org/10.1021/es3027659 CrossRefGoogle Scholar
  46. Xiao Y et al (2015) Pyrosequencing reveals a core community of anodic bacterial biofilms in bioelectrochemical systems from China. Front Microbiol 6:1410CrossRefGoogle Scholar
  47. Yousaf S, Anam M, Ali N (2017) Evaluating the production and bio-stimulating effect of 5-methyl 1, hydroxy phenazine on microbial fuel cell performance. Int J Environ Sci Technol 14(7):1439–1450CrossRefGoogle Scholar
  48. Yates MD et al (2012) Convergent development of anodic bacterial communities in microbial fuel cells. ISME J 6:2002–2013CrossRefGoogle Scholar
  49. Yu C-M, Chen L-C (2009) Turning glucose and starch into electricity with an enzymatic fuel cell engineering in agriculture. Environ Food 2:1–6Google Scholar

Copyright information

© Islamic Azad University (IAU) 2018

Authors and Affiliations

  • Z. Zafar
    • 1
  • K. Ayaz
    • 1
  • M. H. Nasir
    • 2
  • S. Yousaf
    • 1
  • I. Sharafat
    • 1
  • N. Ali
    • 1
    Email author
  1. 1.Department of MicrobiologyQuaid-i-Azam UniversityIslamabadPakistan
  2. 2.Department of ChemistryQuaid-i-Azam UniversityIslamabadPakistan

Personalised recommendations