The effects of Lactobacillus species isolated from traditional dairy products and Urtica extract on gastric cancer cell line
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Lactobacillus is an essential food fermenting organism with a long history in health and healing. Urtica is a perennial herb known for its applications in traditional therapy. The aim of this study is to evaluate the effectiveness of Lactobacillus and Urtica extract on the elimination of gastric cancer cell line simultaneously. In this study, Lactobacillus species were isolated and then Urtica extract was produced; gastric cancer cell line (AGS) was cultured then treated with these bacteria and the Urtica extract. Lactobacillus casei, Lactobacillus acidophilus and Lactobacillus bulgaricus were able to kill 82.97, 84.33 and 83.06% of AGS cell line, respectively. Urtica extract also eliminated 64.84% of AGS cell line. The combination of Lactobacillus bulgaricus and Lactobacillus casei with Urtica extract could decrease 57.57% in concentration of 1 mg/mL and 72.93% in concentration of 10 mg/mL of AGS cell line, respectively. In addition, Lactobacillus casei and Urtica extract significantly destroyed the AGS cell line in all of the concentrations prepared in the study. Lactobacillus and Urtica extract affected and destroyed the gastric cancer cell line. It is concluded that Lactobacillus casei is more effective than the other isolated Lactobacillus which could be used more in the food industry.
KeywordsFermenting organism Food industry Gastric cancer Lactobacillus Urtica extract
This study was supported by the Vice-Chancellor for a research grant of Islamic Azad University of Shiraz (Fars, Iran).
Compliance with ethical standards
All procedures were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1964 and later versions.
- Ali I, Saleem K, Uddin R, Haque A, El-Azzouny A (2010) Natural products: human friendly anticancer medications. Egypt Pharma J 9:133–179Google Scholar
- Ali I, Saleem K, Aboul-Enein HY, Rather A (2011a) Social aspects of cancer genesis. Cancer Ther 8:6–14Google Scholar
- Ali I, Wani WA, Saleem K (2011c) Cancer scenario in India with future perspectives. Cancer Ther 8:56–70Google Scholar
- Fagoonee S, Li H, Zhang H, Altruda F, Pellicano R (2014) Gastric cancer as a stem-cell disease: data and hypotheses. Panminerva Med 56:289–300Google Scholar
- Franz CM, Huch M, Seifert S, Kramlich J, Bub A, Cho G-S, Watzl B (2015) Influence of a probiotic Lactobacillus casei strains on the colonization with potential pathogenic Streptococci and Staphylococcus aureus in the nasopharyngeal space of healthy men with a low baseline NK cell activity. Med Microbiol Immunol 204:527–538CrossRefGoogle Scholar
- Gong Y, Li T, Li S, Jiang Z, Yang Y, Huang J, Liu Z, Sun H (2016) Achieving high yield of lactic acid for antimicrobial characterization in cephalosporin-resistant Lactobacillus by the co-expression of the phosphofructokinase and glucokinase. J Microbiol Biotechnol 26:1148–1161CrossRefGoogle Scholar
- Iravani S (2013) Gastric cancer as a multifactorial disease. Ann Mil Health Sci Res (EMHSR) 11:157–164Google Scholar
- Lee BB, Toh S-L, Ryan S, Simpson JM, Clezy K, Bossa L, Rice SA, Marial O, Weber G, Kaur J (2016) Probiotics [LGG-BB12 or RC14-GR1] versus placebo as prophylaxis for urinary tract infection in persons with spinal cord injury [ProSCIUTTU]: a study protocol for a randomised controlled trial. BMC Urol 16:18CrossRefGoogle Scholar
- Liu Q, Huang X, Yang D, Si T, Pan S, Yang F (2016a) Yield improvement of exopolysaccharides by a screening of the Lactobacillus acidophilus ATCC and optimization of the fermentation and extraction conditions. Excli J 15:119–133Google Scholar
- Matula K, Collie-Duguid E, Murray G, Parikh K, Grabsch H, Tan P, Lalwani S, Garau R, Ong Y, Bain G, Smith AD, Urquhart G, Bielawski J, Finnegan M, Petty R (2015) Regulation of cellular sphingosine-1-phosphate by sphingosine kinase 1 and sphingosine-1-phosphate lyase determines chemotherapy resistance in gastroesophageal cancer. BMC Cancer 15:762CrossRefGoogle Scholar
- Mojarad Khanghah S, Ganbarov K (2014) Lactobacillus with probiotic potential from homemade cheese in Azerbaijan. Bioimpacts 4:49–52Google Scholar
- Rasouli BS, Ghadimi-Darsajini A, Nekouian R, Iragian G-R (2017) In vitro activity of probiotic Lactobacillus reuteri against gastric cancer progression by downregulation of urokinase plasminogen activator/urokinase plasminogen activator receptor gene expression. J Cancer Res Ther 13:246–251CrossRefGoogle Scholar
- Tsai MM, Huang HW, Wang CS, Lee KF, Tsai CY, Lu PH, Chi HC, Lin YH, Kuo LM, Lin KH (2016) MicroRNA-26b inhibits tumor metastasis by targeting the KPNA2/c-jun pathway in human gastric cancer. Oncotarget 7:39511–39526Google Scholar